Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders
Porcine urinary bladder is one of the most used organs to fabricate extracellular matrix (ECM) hydrogel. Although there are two different ECM types inside a bladder, i.e., urinary bladder matrix (UBM) and a subtype ECM (sECM), most studies have only employed UBM for hydrogel fabrication, and overloo...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/11/13/5820 |
| _version_ | 1797529067262574592 |
|---|---|
| author | Chen-Yu Kao Huynh-Quang-Dieu Nguyen Yu-Chuan Weng Yu-Han Hung Chun-Min Lo |
| author_facet | Chen-Yu Kao Huynh-Quang-Dieu Nguyen Yu-Chuan Weng Yu-Han Hung Chun-Min Lo |
| author_sort | Chen-Yu Kao |
| collection | DOAJ |
| description | Porcine urinary bladder is one of the most used organs to fabricate extracellular matrix (ECM) hydrogel. Although there are two different ECM types inside a bladder, i.e., urinary bladder matrix (UBM) and a subtype ECM (sECM), most studies have only employed UBM for hydrogel fabrication, and overlooked the potential use of sECM. In another aspect, the delamination of UBM from bladders is a time-consuming process; consequently, the use of the whole bladder (WB) will likely increase production yield. Therefore, the objective of this study was to fabricate hydrogels from sECM and WB and compare them to UBM. The results indicated that different layers of the bladder shared almost the same biochemical composition. In terms of gelation kinetics, rheology and morphology, although hydrogels from UBM and sECM exhibited some discrepancies, those from UBM and WB interestingly possessed almost the same characteristics. In in vitro studies, all the hydrogels possessed nearly the same biochemical effects towards L929 viability and C2C12 differentiation. These results could preliminarily indicate that the use of sECM should no longer be ignored, and WB could be a promising substitution for UBM hydrogels, eliminating the need for time-consuming delamination processes, as well as increasing the possibility of mass production. |
| first_indexed | 2024-03-10T10:08:12Z |
| format | Article |
| id | doaj.art-1ec3d58b1d12452d932e690f2d46b7ae |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T10:08:12Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-1ec3d58b1d12452d932e690f2d46b7ae2023-11-22T01:22:30ZengMDPI AGApplied Sciences2076-34172021-06-011113582010.3390/app11135820Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine BladdersChen-Yu Kao0Huynh-Quang-Dieu Nguyen1Yu-Chuan Weng2Yu-Han Hung3Chun-Min Lo4Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Da’an Dist., Taipei 10607, TaiwanGraduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, TaiwanSchool of Medicine, National Defense Medical Center, Taipei 11490, TaiwanDepartment of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 11221, TaiwanDepartment of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 11221, TaiwanPorcine urinary bladder is one of the most used organs to fabricate extracellular matrix (ECM) hydrogel. Although there are two different ECM types inside a bladder, i.e., urinary bladder matrix (UBM) and a subtype ECM (sECM), most studies have only employed UBM for hydrogel fabrication, and overlooked the potential use of sECM. In another aspect, the delamination of UBM from bladders is a time-consuming process; consequently, the use of the whole bladder (WB) will likely increase production yield. Therefore, the objective of this study was to fabricate hydrogels from sECM and WB and compare them to UBM. The results indicated that different layers of the bladder shared almost the same biochemical composition. In terms of gelation kinetics, rheology and morphology, although hydrogels from UBM and sECM exhibited some discrepancies, those from UBM and WB interestingly possessed almost the same characteristics. In in vitro studies, all the hydrogels possessed nearly the same biochemical effects towards L929 viability and C2C12 differentiation. These results could preliminarily indicate that the use of sECM should no longer be ignored, and WB could be a promising substitution for UBM hydrogels, eliminating the need for time-consuming delamination processes, as well as increasing the possibility of mass production.https://www.mdpi.com/2076-3417/11/13/5820bladderdelaminationextracellular matrixdecellularizationhydrogelcollagen |
| spellingShingle | Chen-Yu Kao Huynh-Quang-Dieu Nguyen Yu-Chuan Weng Yu-Han Hung Chun-Min Lo Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders Applied Sciences bladder delamination extracellular matrix decellularization hydrogel collagen |
| title | Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders |
| title_full | Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders |
| title_fullStr | Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders |
| title_full_unstemmed | Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders |
| title_short | Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders |
| title_sort | evaluating the effect of tissue selection on the characteristics of extracellular matrix hydrogels from decellularized porcine bladders |
| topic | bladder delamination extracellular matrix decellularization hydrogel collagen |
| url | https://www.mdpi.com/2076-3417/11/13/5820 |
| work_keys_str_mv | AT chenyukao evaluatingtheeffectoftissueselectiononthecharacteristicsofextracellularmatrixhydrogelsfromdecellularizedporcinebladders AT huynhquangdieunguyen evaluatingtheeffectoftissueselectiononthecharacteristicsofextracellularmatrixhydrogelsfromdecellularizedporcinebladders AT yuchuanweng evaluatingtheeffectoftissueselectiononthecharacteristicsofextracellularmatrixhydrogelsfromdecellularizedporcinebladders AT yuhanhung evaluatingtheeffectoftissueselectiononthecharacteristicsofextracellularmatrixhydrogelsfromdecellularizedporcinebladders AT chunminlo evaluatingtheeffectoftissueselectiononthecharacteristicsofextracellularmatrixhydrogelsfromdecellularizedporcinebladders |