Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens
Catalase (CAT) can eliminate oxygen radicals, but it is unclear whether exogenous CAT can protect chickens against deoxynivalenol (DON)-induced oxidative stress. This study aimed to investigate the effects of supplemental CAT on antioxidant property and gut microbiota in DON-exposed broilers. A tota...
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MDPI AG
2022-11-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/12/830 |
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| author | Weiwei Wang Jingqiang Zhu Qingyun Cao Changming Zhang Zemin Dong Dingyuan Feng Hui Ye Jianjun Zuo |
| author_facet | Weiwei Wang Jingqiang Zhu Qingyun Cao Changming Zhang Zemin Dong Dingyuan Feng Hui Ye Jianjun Zuo |
| author_sort | Weiwei Wang |
| collection | DOAJ |
| description | Catalase (CAT) can eliminate oxygen radicals, but it is unclear whether exogenous CAT can protect chickens against deoxynivalenol (DON)-induced oxidative stress. This study aimed to investigate the effects of supplemental CAT on antioxidant property and gut microbiota in DON-exposed broilers. A total of 144 one-day-old Lingnan yellow-feathered male broilers were randomly divided into three groups (six replicates/group): control, DON group, and DON + CAT (DONC) group. The control and DON group received a diet without and with DON contamination, respectively, while the DONC group received a DON-contaminated diet with 200 U/kg CAT added. Parameter analysis was performed on d 21. The results showed that DON-induced liver enlargement (<i>p</i> < 0.05) was blocked by CAT addition, which also normalized the increases (<i>p</i> < 0.05) in hepatic oxidative metabolites contents and caspase-9 expression. Additionally, CAT addition increased (<i>p</i> < 0.05) the jejunal CAT and GSH-Px activities coupled with T-AOC in DON-exposed broilers, as well as the normalized DON-induced reductions (<i>p</i> < 0.05) of jejunal villus height (VH) and its ratio for crypt depth. There was a difference (<i>p</i> < 0.05) in gut microbiota among groups. The DON group was enriched (<i>p</i> < 0.05) with some harmful bacteria (e.g., <i>Proteobacteria</i>, <i>Gammaproteobacteria</i>, <i>Enterobacteriales</i>, <i>Enterobacteriaceae</i>, and <i>Escherichia</i>/<i>Shigella</i>) that elicited negative correlations (<i>p</i> < 0.05) with jejunal CAT activity, and VH. DONC group was differentially enriched (<i>p</i> < 0.05) with certain beneficial bacteria (e.g., <i>Acidobacteriota</i>, <i>Anaerofustis</i>, and <i>Anaerotruncus</i>) that could benefit intestinal antioxidation and morphology. In conclusion, supplemental CAT alleviates DON-induced oxidative stress and intestinal damage in broilers, which can be associated with its ability to improve gut microbiota, aside from its direct oxygen radical-scavenging activity. |
| first_indexed | 2024-03-09T15:46:26Z |
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| spelling | doaj.art-1ec46065d2b94b5084988689e09439f52023-11-24T18:26:30ZengMDPI AGToxins2072-66512022-11-01141283010.3390/toxins14120830Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler ChickensWeiwei Wang0Jingqiang Zhu1Qingyun Cao2Changming Zhang3Zemin Dong4Dingyuan Feng5Hui Ye6Jianjun Zuo7Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCatalase (CAT) can eliminate oxygen radicals, but it is unclear whether exogenous CAT can protect chickens against deoxynivalenol (DON)-induced oxidative stress. This study aimed to investigate the effects of supplemental CAT on antioxidant property and gut microbiota in DON-exposed broilers. A total of 144 one-day-old Lingnan yellow-feathered male broilers were randomly divided into three groups (six replicates/group): control, DON group, and DON + CAT (DONC) group. The control and DON group received a diet without and with DON contamination, respectively, while the DONC group received a DON-contaminated diet with 200 U/kg CAT added. Parameter analysis was performed on d 21. The results showed that DON-induced liver enlargement (<i>p</i> < 0.05) was blocked by CAT addition, which also normalized the increases (<i>p</i> < 0.05) in hepatic oxidative metabolites contents and caspase-9 expression. Additionally, CAT addition increased (<i>p</i> < 0.05) the jejunal CAT and GSH-Px activities coupled with T-AOC in DON-exposed broilers, as well as the normalized DON-induced reductions (<i>p</i> < 0.05) of jejunal villus height (VH) and its ratio for crypt depth. There was a difference (<i>p</i> < 0.05) in gut microbiota among groups. The DON group was enriched (<i>p</i> < 0.05) with some harmful bacteria (e.g., <i>Proteobacteria</i>, <i>Gammaproteobacteria</i>, <i>Enterobacteriales</i>, <i>Enterobacteriaceae</i>, and <i>Escherichia</i>/<i>Shigella</i>) that elicited negative correlations (<i>p</i> < 0.05) with jejunal CAT activity, and VH. DONC group was differentially enriched (<i>p</i> < 0.05) with certain beneficial bacteria (e.g., <i>Acidobacteriota</i>, <i>Anaerofustis</i>, and <i>Anaerotruncus</i>) that could benefit intestinal antioxidation and morphology. In conclusion, supplemental CAT alleviates DON-induced oxidative stress and intestinal damage in broilers, which can be associated with its ability to improve gut microbiota, aside from its direct oxygen radical-scavenging activity.https://www.mdpi.com/2072-6651/14/12/830antioxidant propertycatalasedeoxynivalenolgut microbiotaintestinal health |
| spellingShingle | Weiwei Wang Jingqiang Zhu Qingyun Cao Changming Zhang Zemin Dong Dingyuan Feng Hui Ye Jianjun Zuo Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens Toxins antioxidant property catalase deoxynivalenol gut microbiota intestinal health |
| title | Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens |
| title_full | Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens |
| title_fullStr | Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens |
| title_full_unstemmed | Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens |
| title_short | Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens |
| title_sort | dietary catalase supplementation alleviates deoxynivalenol induced oxidative stress and gut microbiota dysbiosis in broiler chickens |
| topic | antioxidant property catalase deoxynivalenol gut microbiota intestinal health |
| url | https://www.mdpi.com/2072-6651/14/12/830 |
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