Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases
Lipoxygenases (LOXs) catalyze the (per) oxidation of fatty acids that serve as important mediators for cell signaling and inflammation. These reactions are initiated by a C-H activation step that is allosterically regulated in plant and animal enzymes. LOXs from higher eukaryotes are equipped with a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/15/3374 |
| _version_ | 1827712348237856768 |
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| author | Adam R. Offenbacher Theodore R. Holman |
| author_facet | Adam R. Offenbacher Theodore R. Holman |
| author_sort | Adam R. Offenbacher |
| collection | DOAJ |
| description | Lipoxygenases (LOXs) catalyze the (per) oxidation of fatty acids that serve as important mediators for cell signaling and inflammation. These reactions are initiated by a C-H activation step that is allosterically regulated in plant and animal enzymes. LOXs from higher eukaryotes are equipped with an N-terminal PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain that has been implicated to bind to small molecule allosteric effectors, which in turn modulate substrate specificity and the rate-limiting steps of catalysis. Herein, the kinetic and structural evidence that describes the allosteric regulation of plant and animal lipoxygenase chemistry by fatty acids and their derivatives are summarized. |
| first_indexed | 2024-03-10T18:13:42Z |
| format | Article |
| id | doaj.art-1ec4f9a8ce5540799c47a230376470ad |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:13:42Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1ec4f9a8ce5540799c47a230376470ad2023-11-20T07:54:08ZengMDPI AGMolecules1420-30492020-07-012515337410.3390/molecules25153374Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal LipoxygenasesAdam R. Offenbacher0Theodore R. Holman1Department of Chemistry, East Carolina University, Greenville, NC 27858, USADepartment of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, CA 95064, USALipoxygenases (LOXs) catalyze the (per) oxidation of fatty acids that serve as important mediators for cell signaling and inflammation. These reactions are initiated by a C-H activation step that is allosterically regulated in plant and animal enzymes. LOXs from higher eukaryotes are equipped with an N-terminal PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain that has been implicated to bind to small molecule allosteric effectors, which in turn modulate substrate specificity and the rate-limiting steps of catalysis. Herein, the kinetic and structural evidence that describes the allosteric regulation of plant and animal lipoxygenase chemistry by fatty acids and their derivatives are summarized.https://www.mdpi.com/1420-3049/25/15/3374protein allosteryC-H activationkinetic isotope effectssubstrate selectivityhydrogen tunneling |
| spellingShingle | Adam R. Offenbacher Theodore R. Holman Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases Molecules protein allostery C-H activation kinetic isotope effects substrate selectivity hydrogen tunneling |
| title | Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases |
| title_full | Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases |
| title_fullStr | Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases |
| title_full_unstemmed | Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases |
| title_short | Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases |
| title_sort | fatty acid allosteric regulation of c h activation in plant and animal lipoxygenases |
| topic | protein allostery C-H activation kinetic isotope effects substrate selectivity hydrogen tunneling |
| url | https://www.mdpi.com/1420-3049/25/15/3374 |
| work_keys_str_mv | AT adamroffenbacher fattyacidallostericregulationofchactivationinplantandanimallipoxygenases AT theodorerholman fattyacidallostericregulationofchactivationinplantandanimallipoxygenases |