Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes
Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to...
| Asıl Yazarlar: | , , , , |
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| Materyal Türü: | Makale |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
University of Belgrade, University of Novi Sad
2010-01-01
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| Seri Bilgileri: | Archives of Biological Sciences |
| Konular: | |
| Online Erişim: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641001057B.pdf |
| _version_ | 1828739159872765952 |
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| author | Bratić Ana M. Majić Dragana B. Samardžić Jelena T. Kragl M.W. Maksimović Vesna R. |
| author_facet | Bratić Ana M. Majić Dragana B. Samardžić Jelena T. Kragl M.W. Maksimović Vesna R. |
| author_sort | Bratić Ana M. |
| collection | DOAJ |
| description | Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems. |
| first_indexed | 2024-04-13T00:17:39Z |
| format | Article |
| id | doaj.art-1ecd2a0a8ffc4e9890d2e7b51195c5da |
| institution | Directory Open Access Journal |
| issn | 0354-4664 |
| language | English |
| last_indexed | 2024-04-13T00:17:39Z |
| publishDate | 2010-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-1ecd2a0a8ffc4e9890d2e7b51195c5da2022-12-22T03:10:52ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642010-01-01621576210.2298/ABS1001057BTransient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genesBratić Ana M.Majić Dragana B.Samardžić Jelena T.Kragl M.W.Maksimović Vesna R.Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems.http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641001057B.pdfPromotertransient expressionBY2 cellpollen grain |
| spellingShingle | Bratić Ana M. Majić Dragana B. Samardžić Jelena T. Kragl M.W. Maksimović Vesna R. Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes Archives of Biological Sciences Promoter transient expression BY2 cell pollen grain |
| title | Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes |
| title_full | Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes |
| title_fullStr | Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes |
| title_full_unstemmed | Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes |
| title_short | Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes |
| title_sort | transient expression in tobacco bright yellow 2 cells and pollen grains a fast efficient and reliable system for functional promoter analysis of plant genes |
| topic | Promoter transient expression BY2 cell pollen grain |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641001057B.pdf |
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