Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa Barat

<p><strong><span lang="EN-US">Yellow Spot Disease on Sweet Potato in Bogor, West Java</span></strong></p><p>Yellow spot symptom on sweet potato red cultivar was observed in Cikarawang and Leweung kolot area, Bogor regency. Symptomatic leaf samples...

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Main Authors: Anastasya Hondo, Kartika Catur Damaiyanti, Muhammad Fikri Hafizh, Niky Elfa Amanatillah, Tri Asmira Damayanti
Format: Article
Language:Indonesian
Published: Perhimpunan Fitopatologi Indonesia 2018-10-01
Series:Jurnal Fitopatologi Indonesia
Subjects:
Online Access:http://journal.ipb.ac.id/index.php/jfiti/article/view/23332
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author Anastasya Hondo
Kartika Catur Damaiyanti
Muhammad Fikri Hafizh
Niky Elfa Amanatillah
Tri Asmira Damayanti
author_facet Anastasya Hondo
Kartika Catur Damaiyanti
Muhammad Fikri Hafizh
Niky Elfa Amanatillah
Tri Asmira Damayanti
author_sort Anastasya Hondo
collection DOAJ
description <p><strong><span lang="EN-US">Yellow Spot Disease on Sweet Potato in Bogor, West Java</span></strong></p><p>Yellow spot symptom on sweet potato red cultivar was observed in Cikarawang and Leweung kolot area, Bogor regency. Symptomatic leaf samples gave positive reaction in serological detection by DIBA method using polyclonal antiserum for <em>Potyvirus</em>. DNA fragment of ± 700 bp in length was successfully amplified by RT-PCR using degenerate primer for cylindrical inclusion gene (CI) of <em>Potyvirus.  </em>Further nucleotide sequence analysis indicated high homology to <em>Sweet potato feathery </em> <em>mottle virus </em>(SPFMV). The homology of SPFMV Cikarawang (Crkw) isolate ranging from 89–98% to several isolates from other countries with the highest homology to Japan and Spain isolates. SPFMV Crkw isolate was in the same cluster with the Japan and Spain isolates based on phylogenetic analysis of both nucleotide and amino acid sequences. This is the first report of SPFMV on sweet potato in Bogor, West Java, Indonesia.</p>
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spelling doaj.art-1ece09a36b4142a28bb81ab67dbdddd32022-12-22T00:42:20ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792018-10-0114210.14692/jfi.14.2.6914611Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa BaratAnastasya Hondo0Kartika Catur Damaiyanti1Muhammad Fikri Hafizh2Niky Elfa Amanatillah3Tri Asmira Damayanti4Institut Pertanian BogorInstitut Pertanian BogorInstitut Pertanian BogorInstitut Pertanian BogorInstitut Pertanian Bogor<p><strong><span lang="EN-US">Yellow Spot Disease on Sweet Potato in Bogor, West Java</span></strong></p><p>Yellow spot symptom on sweet potato red cultivar was observed in Cikarawang and Leweung kolot area, Bogor regency. Symptomatic leaf samples gave positive reaction in serological detection by DIBA method using polyclonal antiserum for <em>Potyvirus</em>. DNA fragment of ± 700 bp in length was successfully amplified by RT-PCR using degenerate primer for cylindrical inclusion gene (CI) of <em>Potyvirus.  </em>Further nucleotide sequence analysis indicated high homology to <em>Sweet potato feathery </em> <em>mottle virus </em>(SPFMV). The homology of SPFMV Cikarawang (Crkw) isolate ranging from 89–98% to several isolates from other countries with the highest homology to Japan and Spain isolates. SPFMV Crkw isolate was in the same cluster with the Japan and Spain isolates based on phylogenetic analysis of both nucleotide and amino acid sequences. This is the first report of SPFMV on sweet potato in Bogor, West Java, Indonesia.</p>http://journal.ipb.ac.id/index.php/jfiti/article/view/23332cylindrical inclusion, polyclonal antiserum, Potyvirus, sweet potato, Sweet potato feathery mosaic virus
spellingShingle Anastasya Hondo
Kartika Catur Damaiyanti
Muhammad Fikri Hafizh
Niky Elfa Amanatillah
Tri Asmira Damayanti
Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa Barat
Jurnal Fitopatologi Indonesia
cylindrical inclusion, polyclonal antiserum, Potyvirus, sweet potato, Sweet potato feathery mosaic virus
title Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa Barat
title_full Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa Barat
title_fullStr Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa Barat
title_full_unstemmed Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa Barat
title_short Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa Barat
title_sort penyakit bercak kuning ubi jalar di bogor jawa barat
topic cylindrical inclusion, polyclonal antiserum, Potyvirus, sweet potato, Sweet potato feathery mosaic virus
url http://journal.ipb.ac.id/index.php/jfiti/article/view/23332
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