First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone <i>Stomphia coccinea</i>
A novel peptide AnmTX Sco 9a-1 with the β-hairpin fold was isolated from the swimming sea anemone <i>Stomphia coccinea</i> (Actinostolidae family). The peptide consists of 28 amino acid residues, including modified hydroxyproline residue, and its measured molecular mass is 2960 Da. The p...
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MDPI AG
2022-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/11/1705 |
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| author | Rimma S. Kalina Irina N. Gladkikh Anna A. Klimovich Yulia V. Kozhevnikova Aleksandra N. Kvetkina Eugene A. Rogozhin Sergey G. Koshelev Sergey A. Kozlov Elena V. Leychenko |
| author_facet | Rimma S. Kalina Irina N. Gladkikh Anna A. Klimovich Yulia V. Kozhevnikova Aleksandra N. Kvetkina Eugene A. Rogozhin Sergey G. Koshelev Sergey A. Kozlov Elena V. Leychenko |
| author_sort | Rimma S. Kalina |
| collection | DOAJ |
| description | A novel peptide AnmTX Sco 9a-1 with the β-hairpin fold was isolated from the swimming sea anemone <i>Stomphia coccinea</i> (Actinostolidae family). The peptide consists of 28 amino acid residues, including modified hydroxyproline residue, and its measured molecular mass is 2960 Da. The peptide was not toxic on mice; however, it stimulated their exploratory motivation and active search behavior, and demonstrated an anti-anxiety effect. AnmTX Sco 9a-1 at doses of 0.1 and 1 mg/kg reduced the volume of edema during 24 h better than the nonsteroidal anti-inflammatory drug, Diclofenac, at dose of 1 mg/kg in a model of acute local λ-carrageenan-induced inflammation. ELISA analysis of the animal’s blood showed that peptide at a dose of 1 mg/kg reduced the content of tumor necrosis factor-α (TNF-α), a pro-inflammatory mediator responsible in the edema development, up to the level of TNF-α in the intact group. Besides, AnmTX Sco 9a-1 demonstrated a significant analgesic effect on acute pain sensitivity in the carrageenan-induced thermal hyperalgesia model at doses of 0.1 and 1 mg/kg. Activity of AnmTX Sco 9a-1 was shown not to be associated with modulation of nociceptive ASIC channels. |
| first_indexed | 2024-03-09T18:27:04Z |
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| id | doaj.art-1ed0feb49af9480ea3bbcd0e72c885c0 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T18:27:04Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-1ed0feb49af9480ea3bbcd0e72c885c02023-11-24T07:48:05ZengMDPI AGBiomolecules2218-273X2022-11-011211170510.3390/biom12111705First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone <i>Stomphia coccinea</i>Rimma S. Kalina0Irina N. Gladkikh1Anna A. Klimovich2Yulia V. Kozhevnikova3Aleksandra N. Kvetkina4Eugene A. Rogozhin5Sergey G. Koshelev6Sergey A. Kozlov7Elena V. Leychenko8G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 159, Pr. 100 let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 159, Pr. 100 let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 159, Pr. 100 let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 159, Pr. 100 let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 159, Pr. 100 let Vladivostoku, Vladivostok 690022, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 159, Pr. 100 let Vladivostoku, Vladivostok 690022, RussiaA novel peptide AnmTX Sco 9a-1 with the β-hairpin fold was isolated from the swimming sea anemone <i>Stomphia coccinea</i> (Actinostolidae family). The peptide consists of 28 amino acid residues, including modified hydroxyproline residue, and its measured molecular mass is 2960 Da. The peptide was not toxic on mice; however, it stimulated their exploratory motivation and active search behavior, and demonstrated an anti-anxiety effect. AnmTX Sco 9a-1 at doses of 0.1 and 1 mg/kg reduced the volume of edema during 24 h better than the nonsteroidal anti-inflammatory drug, Diclofenac, at dose of 1 mg/kg in a model of acute local λ-carrageenan-induced inflammation. ELISA analysis of the animal’s blood showed that peptide at a dose of 1 mg/kg reduced the content of tumor necrosis factor-α (TNF-α), a pro-inflammatory mediator responsible in the edema development, up to the level of TNF-α in the intact group. Besides, AnmTX Sco 9a-1 demonstrated a significant analgesic effect on acute pain sensitivity in the carrageenan-induced thermal hyperalgesia model at doses of 0.1 and 1 mg/kg. Activity of AnmTX Sco 9a-1 was shown not to be associated with modulation of nociceptive ASIC channels.https://www.mdpi.com/2218-273X/12/11/1705sea anemonebeta-hairpin peptidesEdman sequencinganalgesic and anti-inflammatory activityTNF-α |
| spellingShingle | Rimma S. Kalina Irina N. Gladkikh Anna A. Klimovich Yulia V. Kozhevnikova Aleksandra N. Kvetkina Eugene A. Rogozhin Sergey G. Koshelev Sergey A. Kozlov Elena V. Leychenko First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone <i>Stomphia coccinea</i> Biomolecules sea anemone beta-hairpin peptides Edman sequencing analgesic and anti-inflammatory activity TNF-α |
| title | First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone <i>Stomphia coccinea</i> |
| title_full | First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone <i>Stomphia coccinea</i> |
| title_fullStr | First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone <i>Stomphia coccinea</i> |
| title_full_unstemmed | First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone <i>Stomphia coccinea</i> |
| title_short | First Anti-Inflammatory Peptide AnmTX Sco 9a-1 from the Swimming Sea Anemone <i>Stomphia coccinea</i> |
| title_sort | first anti inflammatory peptide anmtx sco 9a 1 from the swimming sea anemone i stomphia coccinea i |
| topic | sea anemone beta-hairpin peptides Edman sequencing analgesic and anti-inflammatory activity TNF-α |
| url | https://www.mdpi.com/2218-273X/12/11/1705 |
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