Phage integration alters the respiratory strategy of its host
Temperate bacteriophages are viruses that can incorporate their genomes into their bacterial hosts, existing there as prophages that refrain from killing the host cell until induced. Prophages are largely quiescent, but they can alter host phenotype through factors encoded in their genomes (often vi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/49081 |
| _version_ | 1828384522913185792 |
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| author | Jeffrey N Carey Erin L Mettert Daniel R Fishman-Engel Manuela Roggiani Patricia J Kiley Mark Goulian |
| author_facet | Jeffrey N Carey Erin L Mettert Daniel R Fishman-Engel Manuela Roggiani Patricia J Kiley Mark Goulian |
| author_sort | Jeffrey N Carey |
| collection | DOAJ |
| description | Temperate bacteriophages are viruses that can incorporate their genomes into their bacterial hosts, existing there as prophages that refrain from killing the host cell until induced. Prophages are largely quiescent, but they can alter host phenotype through factors encoded in their genomes (often virulence factors) or by disrupting host genes as a result of integration. Here we describe another mechanism by which a prophage can modulate host phenotype. We show that a temperate phage that integrates in Escherichia coli reprograms host regulation of an anaerobic respiratory system, thereby inhibiting a bet hedging strategy. The phage exerts this effect by upregulating a host-encoded signal transduction protein through transcription initiated from a phage-encoded promoter. We further show that this phenomenon occurs not only in a laboratory strain of E. coli, but also in a natural isolate that contains a prophage at this site. |
| first_indexed | 2024-12-10T05:03:41Z |
| format | Article |
| id | doaj.art-1ed3532889a24820af02f7553aea28da |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T05:03:41Z |
| publishDate | 2019-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-1ed3532889a24820af02f7553aea28da2022-12-22T02:01:18ZengeLife Sciences Publications LtdeLife2050-084X2019-10-01810.7554/eLife.49081Phage integration alters the respiratory strategy of its hostJeffrey N Carey0https://orcid.org/0000-0002-5105-0265Erin L Mettert1Daniel R Fishman-Engel2Manuela Roggiani3Patricia J Kiley4Mark Goulian5https://orcid.org/0000-0003-0076-023XGraduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States; School of Veterinary Medicine, University of Pennsylvania, Philadelphia, United StatesDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin, Madison, United StatesDepartment of Biology, University of Pennsylvania, Philadelphia, United StatesDepartment of Biology, University of Pennsylvania, Philadelphia, United StatesDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin, Madison, United StatesGraduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States; Department of Biology, University of Pennsylvania, Philadelphia, United States; Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, United StatesTemperate bacteriophages are viruses that can incorporate their genomes into their bacterial hosts, existing there as prophages that refrain from killing the host cell until induced. Prophages are largely quiescent, but they can alter host phenotype through factors encoded in their genomes (often virulence factors) or by disrupting host genes as a result of integration. Here we describe another mechanism by which a prophage can modulate host phenotype. We show that a temperate phage that integrates in Escherichia coli reprograms host regulation of an anaerobic respiratory system, thereby inhibiting a bet hedging strategy. The phage exerts this effect by upregulating a host-encoded signal transduction protein through transcription initiated from a phage-encoded promoter. We further show that this phenomenon occurs not only in a laboratory strain of E. coli, but also in a natural isolate that contains a prophage at this site.https://elifesciences.org/articles/49081bacteriophagetrimethylamine N-oxidetwo-component signalingbet hedging |
| spellingShingle | Jeffrey N Carey Erin L Mettert Daniel R Fishman-Engel Manuela Roggiani Patricia J Kiley Mark Goulian Phage integration alters the respiratory strategy of its host eLife bacteriophage trimethylamine N-oxide two-component signaling bet hedging |
| title | Phage integration alters the respiratory strategy of its host |
| title_full | Phage integration alters the respiratory strategy of its host |
| title_fullStr | Phage integration alters the respiratory strategy of its host |
| title_full_unstemmed | Phage integration alters the respiratory strategy of its host |
| title_short | Phage integration alters the respiratory strategy of its host |
| title_sort | phage integration alters the respiratory strategy of its host |
| topic | bacteriophage trimethylamine N-oxide two-component signaling bet hedging |
| url | https://elifesciences.org/articles/49081 |
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