RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FID
Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2023-08-01
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| Series: | Química Nova |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000700743&lng=en&tlng=en |
| _version_ | 1797733674411622400 |
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| author | Brenda Lee Simas Porto Isaura Daniele Leite Faria Jéssica Cordeiro Queiroz de Souza Olívia Brito de Oliveira Moreira Marco Antônio Sundfeld da Gama Marcone Augusto Leal de Oliveira |
| author_facet | Brenda Lee Simas Porto Isaura Daniele Leite Faria Jéssica Cordeiro Queiroz de Souza Olívia Brito de Oliveira Moreira Marco Antônio Sundfeld da Gama Marcone Augusto Leal de Oliveira |
| author_sort | Brenda Lee Simas Porto |
| collection | DOAJ |
| description | Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a fame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency. |
| first_indexed | 2024-03-12T12:31:21Z |
| format | Article |
| id | doaj.art-1ed544a895704880af5f2c0314c35b78 |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-03-12T12:31:21Z |
| publishDate | 2023-08-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-1ed544a895704880af5f2c0314c35b782023-08-29T07:45:49ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642023-08-0146774374610.21577/0100-4042.20230026RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FIDBrenda Lee Simas Portohttps://orcid.org/0000-0003-3469-0284Isaura Daniele Leite FariaJéssica Cordeiro Queiroz de Souzahttps://orcid.org/0000-0001-5278-9865Olívia Brito de Oliveira Moreirahttps://orcid.org/0000-0002-8073-4236Marco Antônio Sundfeld da GamaMarcone Augusto Leal de Oliveirahttps://orcid.org/0000-0001-6026-9608Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a fame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000700743&lng=en&tlng=enfast GCCLASLB-IL111fatty acidsmilk |
| spellingShingle | Brenda Lee Simas Porto Isaura Daniele Leite Faria Jéssica Cordeiro Queiroz de Souza Olívia Brito de Oliveira Moreira Marco Antônio Sundfeld da Gama Marcone Augusto Leal de Oliveira RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FID Química Nova fast GC CLA SLB-IL111 fatty acids milk |
| title | RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FID |
| title_full | RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FID |
| title_fullStr | RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FID |
| title_full_unstemmed | RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FID |
| title_short | RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FID |
| title_sort | rapid method for simultaneous determination of cis 9 trans 11 and trans 10 cis 12 conjugated linoleic acid isomers in milk by gc fid |
| topic | fast GC CLA SLB-IL111 fatty acids milk |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000700743&lng=en&tlng=en |
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