Survey of the infant male urobiome and genomic analysis of Actinotignum spp.
Abstract The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present the urobiome of healthy infant males from samples collected by transurethral catheterization. Using a combination of enhanced culture and amplicon sequencing, we id...
| Principais autores: | , , , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
Nature Portfolio
2023-12-01
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| coleção: | npj Biofilms and Microbiomes |
| Acesso em linha: | https://doi.org/10.1038/s41522-023-00457-6 |
| _version_ | 1827604152109236224 |
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| author | Seth A. Reasoner Viktor Flores Gerald Van Horn Grace Morales Leslie M. Peard Benjamin Abelson Carmila Manuel Jessica Lee Bailey Baker Timothy Williams Jonathan E. Schmitz Douglass B. Clayton Maria Hadjifrangiskou |
| author_facet | Seth A. Reasoner Viktor Flores Gerald Van Horn Grace Morales Leslie M. Peard Benjamin Abelson Carmila Manuel Jessica Lee Bailey Baker Timothy Williams Jonathan E. Schmitz Douglass B. Clayton Maria Hadjifrangiskou |
| author_sort | Seth A. Reasoner |
| collection | DOAJ |
| description | Abstract The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present the urobiome of healthy infant males from samples collected by transurethral catheterization. Using a combination of enhanced culture and amplicon sequencing, we identify several common bacterial genera that can be further investigated for their effects on urinary health across the lifespan. Many genera were shared between all samples suggesting a consistent urobiome composition among this cohort. We note that, for this cohort, early life exposures including mode of birth (vaginal vs. Cesarean section), or prior antibiotic exposure did not influence urobiome composition. In addition, we report the isolation of culturable bacteria from the bladders of these infant males, including Actinotignum spp., a bacterial genus that has been associated with urinary tract infections in older male adults. Herein, we isolate and sequence 9 distinct strains of Actinotignum spp. enhancing the genomic knowledge surrounding this genus and opening avenues for delineating the microbiology of this urobiome constituent. Furthermore, we present a framework for using the combination of culture-dependent and sequencing methodologies for uncovering mechanisms in the urobiome. |
| first_indexed | 2024-03-09T05:54:59Z |
| format | Article |
| id | doaj.art-1ed6af42a71546689f8cc90716fda1d5 |
| institution | Directory Open Access Journal |
| issn | 2055-5008 |
| language | English |
| last_indexed | 2024-03-09T05:54:59Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj.art-1ed6af42a71546689f8cc90716fda1d52023-12-03T12:13:59ZengNature Portfolionpj Biofilms and Microbiomes2055-50082023-12-019111010.1038/s41522-023-00457-6Survey of the infant male urobiome and genomic analysis of Actinotignum spp.Seth A. Reasoner0Viktor Flores1Gerald Van Horn2Grace Morales3Leslie M. Peard4Benjamin Abelson5Carmila Manuel6Jessica Lee7Bailey Baker8Timothy Williams9Jonathan E. Schmitz10Douglass B. Clayton11Maria Hadjifrangiskou12Division of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical CenterDivision of Pediatric Urology, Vanderbilt University Medical CenterDivision of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical CenterDivision of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical CenterDivision of Pediatric Urology, Vanderbilt University Medical CenterDivision of Pediatric Urology, Vanderbilt University Medical CenterDivision of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical CenterDivision of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical CenterDivision of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical CenterDivision of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical CenterDivision of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical CenterDivision of Pediatric Urology, Vanderbilt University Medical CenterDivision of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical CenterAbstract The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present the urobiome of healthy infant males from samples collected by transurethral catheterization. Using a combination of enhanced culture and amplicon sequencing, we identify several common bacterial genera that can be further investigated for their effects on urinary health across the lifespan. Many genera were shared between all samples suggesting a consistent urobiome composition among this cohort. We note that, for this cohort, early life exposures including mode of birth (vaginal vs. Cesarean section), or prior antibiotic exposure did not influence urobiome composition. In addition, we report the isolation of culturable bacteria from the bladders of these infant males, including Actinotignum spp., a bacterial genus that has been associated with urinary tract infections in older male adults. Herein, we isolate and sequence 9 distinct strains of Actinotignum spp. enhancing the genomic knowledge surrounding this genus and opening avenues for delineating the microbiology of this urobiome constituent. Furthermore, we present a framework for using the combination of culture-dependent and sequencing methodologies for uncovering mechanisms in the urobiome.https://doi.org/10.1038/s41522-023-00457-6 |
| spellingShingle | Seth A. Reasoner Viktor Flores Gerald Van Horn Grace Morales Leslie M. Peard Benjamin Abelson Carmila Manuel Jessica Lee Bailey Baker Timothy Williams Jonathan E. Schmitz Douglass B. Clayton Maria Hadjifrangiskou Survey of the infant male urobiome and genomic analysis of Actinotignum spp. npj Biofilms and Microbiomes |
| title | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
| title_full | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
| title_fullStr | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
| title_full_unstemmed | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
| title_short | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
| title_sort | survey of the infant male urobiome and genomic analysis of actinotignum spp |
| url | https://doi.org/10.1038/s41522-023-00457-6 |
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