Long noncoding RNA HOTTIP promotes the metastatic potential of ovarian cancer through the regulation of the miR‐615‐3p/SMARCE1 pathway
Abstract Upregulation of lncRNA HOXA transcript at the distal tip (HOTTIP) plays important roles in cancer progression. Nevertheless, its functions in the growth and metastasis of ovarian carcinoma are unknown. In this study, we demonstrated overexpression of HOTTIP in ovarian cancer cell lines and...
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| Format: | Article |
| Language: | English |
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Wiley
2020-12-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12282 |
| _version_ | 1827304123291140096 |
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| author | Hong Wu Hong‐Yan Wei Qian‐Qian Chen |
| author_facet | Hong Wu Hong‐Yan Wei Qian‐Qian Chen |
| author_sort | Hong Wu |
| collection | DOAJ |
| description | Abstract Upregulation of lncRNA HOXA transcript at the distal tip (HOTTIP) plays important roles in cancer progression. Nevertheless, its functions in the growth and metastasis of ovarian carcinoma are unknown. In this study, we demonstrated overexpression of HOTTIP in ovarian cancer cell lines and clinical tissues. Further, we showed that higher level of HOTTIP was associated with poor survival of ovarian cancer patients. Notably, HOTTIP silencing restrained proliferation, migration, and invasiveness of ovarian carcinoma cells. On the other hand, upregulation of HOTTIP remarkably exacerbated the aggressive traits of ovarian carcinoma cells. In addition, HOTTIP served as a sponge of miR‐615‐3p to upregulate SMARCE1 level. Further, upregulation of miR‐615‐3p or downregulation of SMARCE1 reversed the carcinogenic impacts of HOTTIP in ovarian cancer. HOTTIP and miR‐615‐3p expression levels in ovarian cancer cells were negatively correlated, whereas HOTTIP and SMARCE1 expression levels were positively correlated. In nude mice, downregulation of HOTTIP reduced cell growth in vivo. In summary, lncRNA HOTTIP promotes the growth and metastatic phenotypes of ovarian cancer via regulating miR‐615‐3p/SMARCE1 pathway. |
| first_indexed | 2024-04-24T17:24:31Z |
| format | Article |
| id | doaj.art-1ed7d9f67b6a4555a584c1ac0499fc8c |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-04-24T17:24:31Z |
| publishDate | 2020-12-01 |
| publisher | Wiley |
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| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-1ed7d9f67b6a4555a584c1ac0499fc8c2024-03-28T07:06:00ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502020-12-01361297398210.1002/kjm2.12282Long noncoding RNA HOTTIP promotes the metastatic potential of ovarian cancer through the regulation of the miR‐615‐3p/SMARCE1 pathwayHong Wu0Hong‐Yan Wei1Qian‐Qian Chen2Department of Obstetrics Weifang Maternal and Child Health Hospital Weifang Shandong ChinaDepartment of Obstetrics Weifang Maternal and Child Health Hospital Weifang Shandong ChinaDepartment of Obstetrics Weifang Maternal and Child Health Hospital Weifang Shandong ChinaAbstract Upregulation of lncRNA HOXA transcript at the distal tip (HOTTIP) plays important roles in cancer progression. Nevertheless, its functions in the growth and metastasis of ovarian carcinoma are unknown. In this study, we demonstrated overexpression of HOTTIP in ovarian cancer cell lines and clinical tissues. Further, we showed that higher level of HOTTIP was associated with poor survival of ovarian cancer patients. Notably, HOTTIP silencing restrained proliferation, migration, and invasiveness of ovarian carcinoma cells. On the other hand, upregulation of HOTTIP remarkably exacerbated the aggressive traits of ovarian carcinoma cells. In addition, HOTTIP served as a sponge of miR‐615‐3p to upregulate SMARCE1 level. Further, upregulation of miR‐615‐3p or downregulation of SMARCE1 reversed the carcinogenic impacts of HOTTIP in ovarian cancer. HOTTIP and miR‐615‐3p expression levels in ovarian cancer cells were negatively correlated, whereas HOTTIP and SMARCE1 expression levels were positively correlated. In nude mice, downregulation of HOTTIP reduced cell growth in vivo. In summary, lncRNA HOTTIP promotes the growth and metastatic phenotypes of ovarian cancer via regulating miR‐615‐3p/SMARCE1 pathway.https://doi.org/10.1002/kjm2.12282HOTTIPmiR‐615‐3povarian cancerSMARCE1 |
| spellingShingle | Hong Wu Hong‐Yan Wei Qian‐Qian Chen Long noncoding RNA HOTTIP promotes the metastatic potential of ovarian cancer through the regulation of the miR‐615‐3p/SMARCE1 pathway Kaohsiung Journal of Medical Sciences HOTTIP miR‐615‐3p ovarian cancer SMARCE1 |
| title | Long noncoding RNA HOTTIP promotes the metastatic potential of ovarian cancer through the regulation of the miR‐615‐3p/SMARCE1 pathway |
| title_full | Long noncoding RNA HOTTIP promotes the metastatic potential of ovarian cancer through the regulation of the miR‐615‐3p/SMARCE1 pathway |
| title_fullStr | Long noncoding RNA HOTTIP promotes the metastatic potential of ovarian cancer through the regulation of the miR‐615‐3p/SMARCE1 pathway |
| title_full_unstemmed | Long noncoding RNA HOTTIP promotes the metastatic potential of ovarian cancer through the regulation of the miR‐615‐3p/SMARCE1 pathway |
| title_short | Long noncoding RNA HOTTIP promotes the metastatic potential of ovarian cancer through the regulation of the miR‐615‐3p/SMARCE1 pathway |
| title_sort | long noncoding rna hottip promotes the metastatic potential of ovarian cancer through the regulation of the mir 615 3p smarce1 pathway |
| topic | HOTTIP miR‐615‐3p ovarian cancer SMARCE1 |
| url | https://doi.org/10.1002/kjm2.12282 |
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