Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats
MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate several pathway intermediates and affect the skeletal muscle development in mice, pigs, sheep, and cattle. However, to date, only a small number of miRNAs have been reported in the muscle development of goats. In this...
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MDPI AG
2023-01-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/2/315 |
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| author | Sanbao Zhang Qiongwen Zhang Lili Yang Xiaotong Gao Ting Chen Tianbao Li Wenyue Sun Yufan Liu Zihua Zheng Yan Pan Yingming Wei Yanna Huang Mingsheng Jiang Qinyang Jiang |
| author_facet | Sanbao Zhang Qiongwen Zhang Lili Yang Xiaotong Gao Ting Chen Tianbao Li Wenyue Sun Yufan Liu Zihua Zheng Yan Pan Yingming Wei Yanna Huang Mingsheng Jiang Qinyang Jiang |
| author_sort | Sanbao Zhang |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate several pathway intermediates and affect the skeletal muscle development in mice, pigs, sheep, and cattle. However, to date, only a small number of miRNAs have been reported in the muscle development of goats. In this report, the <i>longissimus dorsi</i> transcripts of one- and ten-month-old goats were analyzed by sequencing RNAs and miRNAs. The results showed that the ten-month-old Longlin goats had 327 up- and 419 down-regulated differentially expressed genes (DEGs) compared with the one-month-old. In addition, 20 co-up-regulated and 55 co-down-regulated miRNAs involved in the muscle fiber hypertrophy of goats were identified in ten-month-old Longlin and Nubian goats compared with one-month-old. Five miRNA–mRNA pairs (<i>chi-let-7b-3p-MIRLET7A</i>, <i>chi-miR193b-3p-MMP14</i>, <i>chi-miR-355-5p-DGAT2</i>, <i>novel_128-LOC102178119</i>, <i>novel_140-SOD3</i>) involved in the goat skeletal muscle development were identified by miRNA–mRNA negative correlation network analysis. Our results provided new insight into the functional roles of goat muscle-associated miRNAs, allowing a deeper understanding of the transformation of miRNA roles during mammalian muscle development. |
| first_indexed | 2024-03-11T08:46:36Z |
| format | Article |
| id | doaj.art-1ed825d35db14cb39eab07804117f1d6 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T08:46:36Z |
| publishDate | 2023-01-01 |
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| series | Genes |
| spelling | doaj.art-1ed825d35db14cb39eab07804117f1d62023-11-16T20:41:16ZengMDPI AGGenes2073-44252023-01-0114231510.3390/genes14020315Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult GoatsSanbao Zhang0Qiongwen Zhang1Lili Yang2Xiaotong Gao3Ting Chen4Tianbao Li5Wenyue Sun6Yufan Liu7Zihua Zheng8Yan Pan9Yingming Wei10Yanna Huang11Mingsheng Jiang12Qinyang Jiang13College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaInstitute for New Rural Development, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi Agricultural Vocational University, Nanning 530007, ChinaInstitute for New Rural Development, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaMicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate several pathway intermediates and affect the skeletal muscle development in mice, pigs, sheep, and cattle. However, to date, only a small number of miRNAs have been reported in the muscle development of goats. In this report, the <i>longissimus dorsi</i> transcripts of one- and ten-month-old goats were analyzed by sequencing RNAs and miRNAs. The results showed that the ten-month-old Longlin goats had 327 up- and 419 down-regulated differentially expressed genes (DEGs) compared with the one-month-old. In addition, 20 co-up-regulated and 55 co-down-regulated miRNAs involved in the muscle fiber hypertrophy of goats were identified in ten-month-old Longlin and Nubian goats compared with one-month-old. Five miRNA–mRNA pairs (<i>chi-let-7b-3p-MIRLET7A</i>, <i>chi-miR193b-3p-MMP14</i>, <i>chi-miR-355-5p-DGAT2</i>, <i>novel_128-LOC102178119</i>, <i>novel_140-SOD3</i>) involved in the goat skeletal muscle development were identified by miRNA–mRNA negative correlation network analysis. Our results provided new insight into the functional roles of goat muscle-associated miRNAs, allowing a deeper understanding of the transformation of miRNA roles during mammalian muscle development.https://www.mdpi.com/2073-4425/14/2/315miRNAmRNAmuscle fiberhypertrophygoat |
| spellingShingle | Sanbao Zhang Qiongwen Zhang Lili Yang Xiaotong Gao Ting Chen Tianbao Li Wenyue Sun Yufan Liu Zihua Zheng Yan Pan Yingming Wei Yanna Huang Mingsheng Jiang Qinyang Jiang Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats Genes miRNA mRNA muscle fiber hypertrophy goat |
| title | Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats |
| title_full | Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats |
| title_fullStr | Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats |
| title_full_unstemmed | Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats |
| title_short | Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats |
| title_sort | comparative and functional analysis of mirnas and mrnas involved in muscle fiber hypertrophy of juvenile and adult goats |
| topic | miRNA mRNA muscle fiber hypertrophy goat |
| url | https://www.mdpi.com/2073-4425/14/2/315 |
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