Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor Angiogenesis
Bone marrow contains endothelial progenitor cells (EPCs) that, upon pro-angiogenic stimuli, migrate and differentiate into endothelial cells (ECs) and contribute to re-endothelialization and neo-vascularization. There are currently no reliable markers to characterize EPCs, leading to their inaccurat...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/9/1/107 |
| _version_ | 1827849640343502848 |
|---|---|
| author | Filipa Lopes-Coelho Fernanda Silva Sofia Gouveia-Fernandes Carmo Martins Nuno Lopes Germana Domingues Catarina Brito António M Almeida Sofia A Pereira Jacinta Serpa |
| author_facet | Filipa Lopes-Coelho Fernanda Silva Sofia Gouveia-Fernandes Carmo Martins Nuno Lopes Germana Domingues Catarina Brito António M Almeida Sofia A Pereira Jacinta Serpa |
| author_sort | Filipa Lopes-Coelho |
| collection | DOAJ |
| description | Bone marrow contains endothelial progenitor cells (EPCs) that, upon pro-angiogenic stimuli, migrate and differentiate into endothelial cells (ECs) and contribute to re-endothelialization and neo-vascularization. There are currently no reliable markers to characterize EPCs, leading to their inaccurate identification. In the past, we showed that, in a panel of tumors, some cells on the vessel wall co-expressed CD14 (monocytic marker) and CD31 (EC marker), indicating a putative differentiation route of monocytes into ECs. Herein, we disclosed monocytes as potential EPCs, using in vitro and in vivo models, and also addressed the cancer context. Monocytes acquired the capacity to express ECs markers and were able to be incorporated into blood vessels, contributing to cancer progression, by being incorporated in tumor neo-vasculature. Reactive oxygen species (ROS) push monocytes to EC differentiation, and this phenotype is reverted by cysteine (a scavenger and precursor of glutathione), which indicates that angiogenesis is controlled by the interplay between the oxidative stress and the scavenging capacity of the tumor microenvironment. |
| first_indexed | 2024-03-12T10:08:09Z |
| format | Article |
| id | doaj.art-1edc457f1de94b7bb2d46f66adea26fe |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T10:08:09Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-1edc457f1de94b7bb2d46f66adea26fe2023-09-02T11:02:42ZengMDPI AGCells2073-44092020-01-019110710.3390/cells9010107cells9010107Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor AngiogenesisFilipa Lopes-Coelho0Fernanda Silva1Sofia Gouveia-Fernandes2Carmo Martins3Nuno Lopes4Germana Domingues5Catarina Brito6António M Almeida7Sofia A Pereira8Jacinta Serpa9CEDOC, Chronic Diseases Research Centre, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria, 130, 1169-056 Lisboa, PortugalCEDOC, Chronic Diseases Research Centre, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria, 130, 1169-056 Lisboa, PortugalCEDOC, Chronic Diseases Research Centre, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria, 130, 1169-056 Lisboa, PortugalInstituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG), Rua Prof. Lima Basto 1099-023 Lisboa, PortugalInstituto de Biologia Experimental e Tecnológica, Avenida da República, Estação Agronómica, 2780-157 Oeiras, PortugalCEDOC, Chronic Diseases Research Centre, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria, 130, 1169-056 Lisboa, PortugalInstituto de Biologia Experimental e Tecnológica, Avenida da República, Estação Agronómica, 2780-157 Oeiras, PortugalInstituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG), Rua Prof. Lima Basto 1099-023 Lisboa, PortugalCEDOC, Chronic Diseases Research Centre, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria, 130, 1169-056 Lisboa, PortugalCEDOC, Chronic Diseases Research Centre, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria, 130, 1169-056 Lisboa, PortugalBone marrow contains endothelial progenitor cells (EPCs) that, upon pro-angiogenic stimuli, migrate and differentiate into endothelial cells (ECs) and contribute to re-endothelialization and neo-vascularization. There are currently no reliable markers to characterize EPCs, leading to their inaccurate identification. In the past, we showed that, in a panel of tumors, some cells on the vessel wall co-expressed CD14 (monocytic marker) and CD31 (EC marker), indicating a putative differentiation route of monocytes into ECs. Herein, we disclosed monocytes as potential EPCs, using in vitro and in vivo models, and also addressed the cancer context. Monocytes acquired the capacity to express ECs markers and were able to be incorporated into blood vessels, contributing to cancer progression, by being incorporated in tumor neo-vasculature. Reactive oxygen species (ROS) push monocytes to EC differentiation, and this phenotype is reverted by cysteine (a scavenger and precursor of glutathione), which indicates that angiogenesis is controlled by the interplay between the oxidative stress and the scavenging capacity of the tumor microenvironment.https://www.mdpi.com/2073-4409/9/1/107monocytesendothelial progenitor cells (epcs)endothelial cells (ecs)angiogenesiscancer |
| spellingShingle | Filipa Lopes-Coelho Fernanda Silva Sofia Gouveia-Fernandes Carmo Martins Nuno Lopes Germana Domingues Catarina Brito António M Almeida Sofia A Pereira Jacinta Serpa Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor Angiogenesis Cells monocytes endothelial progenitor cells (epcs) endothelial cells (ecs) angiogenesis cancer |
| title | Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor Angiogenesis |
| title_full | Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor Angiogenesis |
| title_fullStr | Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor Angiogenesis |
| title_full_unstemmed | Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor Angiogenesis |
| title_short | Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor Angiogenesis |
| title_sort | monocytes as endothelial progenitor cells epcs another brick in the wall to disentangle tumor angiogenesis |
| topic | monocytes endothelial progenitor cells (epcs) endothelial cells (ecs) angiogenesis cancer |
| url | https://www.mdpi.com/2073-4409/9/1/107 |
| work_keys_str_mv | AT filipalopescoelho monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis AT fernandasilva monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis AT sofiagouveiafernandes monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis AT carmomartins monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis AT nunolopes monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis AT germanadomingues monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis AT catarinabrito monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis AT antoniomalmeida monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis AT sofiaapereira monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis AT jacintaserpa monocytesasendothelialprogenitorcellsepcsanotherbrickinthewalltodisentangletumorangiogenesis |