α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2
In this work six psychophilic and psychrotrophic bacterial strains were screened for the presence of different glycosidase activities (α-galactosidase, α-glucosidase, β-glucosidase, α-mannosidase and β-glucuronidase). Nine enzymes were found and their elementary characteristics were measured (toptim...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2005-06-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200503-0005_alpha-glucosidase-and-beta-glucosidase-from-psychrotrophic-strain-arthrobacter-sp-c2-2.php |
| _version_ | 1828009214095654912 |
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| author | Eva Benešová Michaela Marková Blanka Králová |
| author_facet | Eva Benešová Michaela Marková Blanka Králová |
| author_sort | Eva Benešová |
| collection | DOAJ |
| description | In this work six psychophilic and psychrotrophic bacterial strains were screened for the presence of different glycosidase activities (α-galactosidase, α-glucosidase, β-glucosidase, α-mannosidase and β-glucuronidase). Nine enzymes were found and their elementary characteristics were measured (toptimum, pHoptimum, Km, Vlim).Two enzymes with the highest activities at low temperatures were chosen for the next study, i.e. α-glucosidase and β-glucosidase from the psychrotrophic strain Arthrobacter sp. C2-2. These enzymes were purified by ammonium sulphate precipitation, by chromatography with hydrophobic interaction, and by ion-exchange chromatography. Their molecular weights (α-glucosidase - 76 kDa, β-glucosidase - 93 kDa) were determined by gel chromatography. In addition to this, it was verified that both of these enzymes are able to catalyse the transglycosylation reaction with the saccharidic donor and acceptor. |
| first_indexed | 2024-04-10T08:35:34Z |
| format | Article |
| id | doaj.art-1edcae6d0e89449dbc7114dead1c4521 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:34Z |
| publishDate | 2005-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-1edcae6d0e89449dbc7114dead1c45212023-02-23T03:26:58ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172005-06-0123311612010.17221/3380-CJFScjf-200503-0005α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2Eva Benešová0Michaela Marková1Blanka Králová2Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech RepublicIn this work six psychophilic and psychrotrophic bacterial strains were screened for the presence of different glycosidase activities (α-galactosidase, α-glucosidase, β-glucosidase, α-mannosidase and β-glucuronidase). Nine enzymes were found and their elementary characteristics were measured (toptimum, pHoptimum, Km, Vlim).Two enzymes with the highest activities at low temperatures were chosen for the next study, i.e. α-glucosidase and β-glucosidase from the psychrotrophic strain Arthrobacter sp. C2-2. These enzymes were purified by ammonium sulphate precipitation, by chromatography with hydrophobic interaction, and by ion-exchange chromatography. Their molecular weights (α-glucosidase - 76 kDa, β-glucosidase - 93 kDa) were determined by gel chromatography. In addition to this, it was verified that both of these enzymes are able to catalyse the transglycosylation reaction with the saccharidic donor and acceptor.https://cjfs.agriculturejournals.cz/artkey/cjf-200503-0005_alpha-glucosidase-and-beta-glucosidase-from-psychrotrophic-strain-arthrobacter-sp-c2-2.phpcold-active enzymeglycosidasetransglycosylation |
| spellingShingle | Eva Benešová Michaela Marková Blanka Králová α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2 Czech Journal of Food Sciences cold-active enzyme glycosidase transglycosylation |
| title | α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2 |
| title_full | α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2 |
| title_fullStr | α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2 |
| title_full_unstemmed | α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2 |
| title_short | α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2 |
| title_sort | α glucosidase and β glucosidase from psychrotrophic strain arthrobacter sp c2 2 |
| topic | cold-active enzyme glycosidase transglycosylation |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200503-0005_alpha-glucosidase-and-beta-glucosidase-from-psychrotrophic-strain-arthrobacter-sp-c2-2.php |
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