Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using <i>Punica granatum</i> Leaves
The current research work illustrates an economical and rapid approach towards the biogenic synthesis of silver nanoparticles using aqueous <i>Punica granatum</i> leaves extract (PGL-AgNPs). The optimization of major parameters involved in the biosynthesis process was done using Box-Behn...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/19/5762 |
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| author | Monisha Singhal Sreemoyee Chatterjee Ajeet Kumar Asad Syed Ali H. Bahkali Nidhi Gupta Surendra Nimesh |
| author_facet | Monisha Singhal Sreemoyee Chatterjee Ajeet Kumar Asad Syed Ali H. Bahkali Nidhi Gupta Surendra Nimesh |
| author_sort | Monisha Singhal |
| collection | DOAJ |
| description | The current research work illustrates an economical and rapid approach towards the biogenic synthesis of silver nanoparticles using aqueous <i>Punica granatum</i> leaves extract (PGL-AgNPs). The optimization of major parameters involved in the biosynthesis process was done using Box-Behnken Design (BBD). The effects of different independent variables (parameters), namely concentration of AgNO<sub>3</sub>, temperature and ratio of extract to AgNO<sub>3,</sub> on response viz. particle size and polydispersity index were analyzed. As a result of experiment designing, 17 reactions were generated, which were further validated experimentally. The statistical and mathematical approaches were employed on these reactions in order to interpret the relationship between the factors and responses. The biosynthesized nanoparticles were initially characterized by UV-vis spectrophotometry followed by physicochemical analysis for determination of particle size, polydispersity index and zeta potential via dynamic light scattering (DLS), SEM and EDX studies. Moreover, the determination of the functional group present in the leaves extract and PGL-AgNPs was done by FTIR. Antibacterial and antibiofilm efficacies of PGL-AgNPs against Gram-positive and Gram-negative bacteria were further determined. The physicochemical studies suggested that PGL-AgNPs were round in shape and of ~37.5 nm in size with uniform distribution. Our studies suggested that PGL-AgNPs exhibit potent antibacterial and antibiofilm properties. |
| first_indexed | 2024-03-10T06:54:43Z |
| format | Article |
| id | doaj.art-1edce4b809a342c3800f26dec77c82a4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:54:43Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1edce4b809a342c3800f26dec77c82a42023-11-22T16:32:14ZengMDPI AGMolecules1420-30492021-09-012619576210.3390/molecules26195762Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using <i>Punica granatum</i> LeavesMonisha Singhal0Sreemoyee Chatterjee1Ajeet Kumar2Asad Syed3Ali H. Bahkali4Nidhi Gupta5Surendra Nimesh6Department of Biotechnology, IIS (Deemed to be University), Gurukul Marg, SFS, Mansarovar, Jaipur 302020, IndiaDepartment of Biotechnology, IIS (Deemed to be University), Gurukul Marg, SFS, Mansarovar, Jaipur 302020, IndiaDepartment of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5814, USADepartment of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi ArabiaDepartment of Biotechnology, IIS (Deemed to be University), Gurukul Marg, SFS, Mansarovar, Jaipur 302020, IndiaDepartment of Biotechnology, School of Life Sciences, Central University of Rajasthan, Ajmer 305817, IndiaThe current research work illustrates an economical and rapid approach towards the biogenic synthesis of silver nanoparticles using aqueous <i>Punica granatum</i> leaves extract (PGL-AgNPs). The optimization of major parameters involved in the biosynthesis process was done using Box-Behnken Design (BBD). The effects of different independent variables (parameters), namely concentration of AgNO<sub>3</sub>, temperature and ratio of extract to AgNO<sub>3,</sub> on response viz. particle size and polydispersity index were analyzed. As a result of experiment designing, 17 reactions were generated, which were further validated experimentally. The statistical and mathematical approaches were employed on these reactions in order to interpret the relationship between the factors and responses. The biosynthesized nanoparticles were initially characterized by UV-vis spectrophotometry followed by physicochemical analysis for determination of particle size, polydispersity index and zeta potential via dynamic light scattering (DLS), SEM and EDX studies. Moreover, the determination of the functional group present in the leaves extract and PGL-AgNPs was done by FTIR. Antibacterial and antibiofilm efficacies of PGL-AgNPs against Gram-positive and Gram-negative bacteria were further determined. The physicochemical studies suggested that PGL-AgNPs were round in shape and of ~37.5 nm in size with uniform distribution. Our studies suggested that PGL-AgNPs exhibit potent antibacterial and antibiofilm properties.https://www.mdpi.com/1420-3049/26/19/5762<i>Punica granatum</i> leaves extractsilver nanoparticlesBox-Behnken designantibacterialantibiofilm |
| spellingShingle | Monisha Singhal Sreemoyee Chatterjee Ajeet Kumar Asad Syed Ali H. Bahkali Nidhi Gupta Surendra Nimesh Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using <i>Punica granatum</i> Leaves Molecules <i>Punica granatum</i> leaves extract silver nanoparticles Box-Behnken design antibacterial antibiofilm |
| title | Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using <i>Punica granatum</i> Leaves |
| title_full | Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using <i>Punica granatum</i> Leaves |
| title_fullStr | Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using <i>Punica granatum</i> Leaves |
| title_full_unstemmed | Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using <i>Punica granatum</i> Leaves |
| title_short | Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using <i>Punica granatum</i> Leaves |
| title_sort | exploring the antibacterial and antibiofilm efficacy of silver nanoparticles biosynthesized using i punica granatum i leaves |
| topic | <i>Punica granatum</i> leaves extract silver nanoparticles Box-Behnken design antibacterial antibiofilm |
| url | https://www.mdpi.com/1420-3049/26/19/5762 |
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