Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in <i>Ilex paraguariensis</i> Roots
The appearance of water stress episodes triggers leaf abscission and decreases <i>Ilex paraguariensis</i> yield. To explore the mechanisms that allow it to overcome dehydration, we investigated how the root gene expression varied between water-stressed and non-stressed plants and how the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/13/2404 |
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| author | Edgardo H. Avico Raúl M. Acevedo María J. Duarte Acácio Rodrigues Salvador Adriano Nunes-Nesi Oscar A. Ruiz Pedro A. Sansberro |
| author_facet | Edgardo H. Avico Raúl M. Acevedo María J. Duarte Acácio Rodrigues Salvador Adriano Nunes-Nesi Oscar A. Ruiz Pedro A. Sansberro |
| author_sort | Edgardo H. Avico |
| collection | DOAJ |
| description | The appearance of water stress episodes triggers leaf abscission and decreases <i>Ilex paraguariensis</i> yield. To explore the mechanisms that allow it to overcome dehydration, we investigated how the root gene expression varied between water-stressed and non-stressed plants and how the modulation of gene expression was linked to metabolite composition and physiological status. After water deprivation, 5160 differentially expressed transcripts were obtained through RNA-seq. The functional enrichment of induced transcripts revealed significant transcriptional remodelling of stress-related perception, signalling, transcription, and metabolism. Simultaneously, the induction of the enzyme 9-cis-expoxycarotenoid dioxygenase (NCED) transcripts reflected the central role of the hormone abscisic acid in this response. Consequently, the total content of amino acids and soluble sugars increased, and that of starch decreased. Likewise, osmotic adjustment and radical growth were significantly promoted to preserve cell membranes and water uptake. This study provides a valuable resource for future research to understand the molecular adaptation of <i>I. paraguariensis</i> plants under drought conditions and facilitates the exploration of drought-tolerant candidate genes. |
| first_indexed | 2024-03-11T01:32:00Z |
| format | Article |
| id | doaj.art-1ee5456e6c66496f8a414c5105ac0b12 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T01:32:00Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1ee5456e6c66496f8a414c5105ac0b122023-11-18T17:16:57ZengMDPI AGPlants2223-77472023-06-011213240410.3390/plants12132404Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in <i>Ilex paraguariensis</i> RootsEdgardo H. Avico0Raúl M. Acevedo1María J. Duarte2Acácio Rodrigues Salvador3Adriano Nunes-Nesi4Oscar A. Ruiz5Pedro A. Sansberro6Laboratorio de Biotecnología Aplicada y Genómica Funcional, Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sgto. Cabral 2131, Corrientes W3402BKG, ArgentinaLaboratorio de Biotecnología Aplicada y Genómica Funcional, Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sgto. Cabral 2131, Corrientes W3402BKG, ArgentinaLaboratorio de Biotecnología Aplicada y Genómica Funcional, Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sgto. Cabral 2131, Corrientes W3402BKG, ArgentinaNational Institute of Science and Technology on Plant Physiology under Stress Conditions, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilNational Institute of Science and Technology on Plant Physiology under Stress Conditions, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilUnidad de Biotecnología 1, IIB-INTECH (UNSAM-CONICET), Chascomús B7130IWA, ArgentinaLaboratorio de Biotecnología Aplicada y Genómica Funcional, Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sgto. Cabral 2131, Corrientes W3402BKG, ArgentinaThe appearance of water stress episodes triggers leaf abscission and decreases <i>Ilex paraguariensis</i> yield. To explore the mechanisms that allow it to overcome dehydration, we investigated how the root gene expression varied between water-stressed and non-stressed plants and how the modulation of gene expression was linked to metabolite composition and physiological status. After water deprivation, 5160 differentially expressed transcripts were obtained through RNA-seq. The functional enrichment of induced transcripts revealed significant transcriptional remodelling of stress-related perception, signalling, transcription, and metabolism. Simultaneously, the induction of the enzyme 9-cis-expoxycarotenoid dioxygenase (NCED) transcripts reflected the central role of the hormone abscisic acid in this response. Consequently, the total content of amino acids and soluble sugars increased, and that of starch decreased. Likewise, osmotic adjustment and radical growth were significantly promoted to preserve cell membranes and water uptake. This study provides a valuable resource for future research to understand the molecular adaptation of <i>I. paraguariensis</i> plants under drought conditions and facilitates the exploration of drought-tolerant candidate genes.https://www.mdpi.com/2223-7747/12/13/2404<i>Ilex paraguariensis</i>drought stress responseroot transcriptomeRNA-seq |
| spellingShingle | Edgardo H. Avico Raúl M. Acevedo María J. Duarte Acácio Rodrigues Salvador Adriano Nunes-Nesi Oscar A. Ruiz Pedro A. Sansberro Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in <i>Ilex paraguariensis</i> Roots Plants <i>Ilex paraguariensis</i> drought stress response root transcriptome RNA-seq |
| title | Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in <i>Ilex paraguariensis</i> Roots |
| title_full | Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in <i>Ilex paraguariensis</i> Roots |
| title_fullStr | Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in <i>Ilex paraguariensis</i> Roots |
| title_full_unstemmed | Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in <i>Ilex paraguariensis</i> Roots |
| title_short | Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in <i>Ilex paraguariensis</i> Roots |
| title_sort | integrating transcriptional metabolic and physiological responses to drought stress in i ilex paraguariensis i roots |
| topic | <i>Ilex paraguariensis</i> drought stress response root transcriptome RNA-seq |
| url | https://www.mdpi.com/2223-7747/12/13/2404 |
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