Complex formation of potassium salt of highly fatty acid with hemagglutinin protein in influenza virus via exothermic interaction
In our previous study, we found highly fatty acid salts, which are a skin-friendly soaps, had a high ability to inactivate the influenza virus. In order to elucidate the mechanism of inactivation of influenza virus, we investigated interactions and complex formation of potassium tetradecanoate (C14K...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580822001029 |
| _version_ | 1817998579618283520 |
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| author | Takayoshi Kawahara Megumi Sakou Yukie Fumotogawa Satoshi Kanazawa Takemasa Sakaguchi Isamu Akiba |
| author_facet | Takayoshi Kawahara Megumi Sakou Yukie Fumotogawa Satoshi Kanazawa Takemasa Sakaguchi Isamu Akiba |
| author_sort | Takayoshi Kawahara |
| collection | DOAJ |
| description | In our previous study, we found highly fatty acid salts, which are a skin-friendly soaps, had a high ability to inactivate the influenza virus. In order to elucidate the mechanism of inactivation of influenza virus, we investigated interactions and complex formation of potassium tetradecanoate (C14K) as a highly fatty acid salt with a virus particle (VP) derived from avian influenza virus by using isothermal titration calorimetry (ITC) and small-angle X-ray scattering (SAXS). ITC showed C14K attractively interacted with hemagglutinin protein (HA) which exists in the envelop of VP. SAXS analyses revealed C14K formed highly ordered complex with HA through the attractive interaction. Since the HA is responsible for cell entry events, inactivation of influenza viruses by highly fatty acid salts are derived owing to HA inhibition of influenza viruses through the complex formation. Time-resolved SAXS measurements elucidated the complex formation was completed within 40 s after mixing aqueous solutions of C14K and VP. This result strongly suggests that hand-washing with a highly fatty acid salts is an effective measure to prevent infection with influenza virus without causing rough hands. |
| first_indexed | 2024-04-14T02:55:26Z |
| format | Article |
| id | doaj.art-1ee5d407c9bc4695b2bd8938959d3cd5 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-04-14T02:55:26Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-1ee5d407c9bc4695b2bd8938959d3cd52022-12-22T02:16:07ZengElsevierBiochemistry and Biophysics Reports2405-58082022-09-0131101302Complex formation of potassium salt of highly fatty acid with hemagglutinin protein in influenza virus via exothermic interactionTakayoshi Kawahara0Megumi Sakou1Yukie Fumotogawa2Satoshi Kanazawa3Takemasa Sakaguchi4Isamu Akiba5Research and Development Department, Shabondama Soap Co., Ltd., 2-23-1 Minamifutashima, Wakamatsu, Kitakyushu, 808-0195, JapanDepartment of Chemistry and Biochemistry, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, 808-0135, JapanDepartment of Chemistry and Biochemistry, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, 808-0135, JapanDepartment of Chemistry and Biochemistry, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, 808-0135, JapanDepartment of Virology, Institute of Biomedical and Health Science, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, JapanDepartment of Chemistry and Biochemistry, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, 808-0135, Japan; Corresponding author.In our previous study, we found highly fatty acid salts, which are a skin-friendly soaps, had a high ability to inactivate the influenza virus. In order to elucidate the mechanism of inactivation of influenza virus, we investigated interactions and complex formation of potassium tetradecanoate (C14K) as a highly fatty acid salt with a virus particle (VP) derived from avian influenza virus by using isothermal titration calorimetry (ITC) and small-angle X-ray scattering (SAXS). ITC showed C14K attractively interacted with hemagglutinin protein (HA) which exists in the envelop of VP. SAXS analyses revealed C14K formed highly ordered complex with HA through the attractive interaction. Since the HA is responsible for cell entry events, inactivation of influenza viruses by highly fatty acid salts are derived owing to HA inhibition of influenza viruses through the complex formation. Time-resolved SAXS measurements elucidated the complex formation was completed within 40 s after mixing aqueous solutions of C14K and VP. This result strongly suggests that hand-washing with a highly fatty acid salts is an effective measure to prevent infection with influenza virus without causing rough hands.http://www.sciencedirect.com/science/article/pii/S2405580822001029Natural soapInfluenza virusHemagglutinin proteinSmall-angle X-ray scatteringIsothermal titration calorimetry |
| spellingShingle | Takayoshi Kawahara Megumi Sakou Yukie Fumotogawa Satoshi Kanazawa Takemasa Sakaguchi Isamu Akiba Complex formation of potassium salt of highly fatty acid with hemagglutinin protein in influenza virus via exothermic interaction Biochemistry and Biophysics Reports Natural soap Influenza virus Hemagglutinin protein Small-angle X-ray scattering Isothermal titration calorimetry |
| title | Complex formation of potassium salt of highly fatty acid with hemagglutinin protein in influenza virus via exothermic interaction |
| title_full | Complex formation of potassium salt of highly fatty acid with hemagglutinin protein in influenza virus via exothermic interaction |
| title_fullStr | Complex formation of potassium salt of highly fatty acid with hemagglutinin protein in influenza virus via exothermic interaction |
| title_full_unstemmed | Complex formation of potassium salt of highly fatty acid with hemagglutinin protein in influenza virus via exothermic interaction |
| title_short | Complex formation of potassium salt of highly fatty acid with hemagglutinin protein in influenza virus via exothermic interaction |
| title_sort | complex formation of potassium salt of highly fatty acid with hemagglutinin protein in influenza virus via exothermic interaction |
| topic | Natural soap Influenza virus Hemagglutinin protein Small-angle X-ray scattering Isothermal titration calorimetry |
| url | http://www.sciencedirect.com/science/article/pii/S2405580822001029 |
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