Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim
ABSTRACT: One hundred and twenty chicken samples from feces (n = 80), the carcass surface at slaughter at 2 meat chicken farms (n = 20), and retail chicken meat from 5 markets (n = 20) collected during 2018 and 2019 were examined for the prevalence of plasmid-mediated quinolone resistance (PMQR) in...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121005605 |
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| author | Toshiyuki Murase Patchara Phuektes Hiroichi Ozaki Sunpetch Angkititrakul |
| author_facet | Toshiyuki Murase Patchara Phuektes Hiroichi Ozaki Sunpetch Angkititrakul |
| author_sort | Toshiyuki Murase |
| collection | DOAJ |
| description | ABSTRACT: One hundred and twenty chicken samples from feces (n = 80), the carcass surface at slaughter at 2 meat chicken farms (n = 20), and retail chicken meat from 5 markets (n = 20) collected during 2018 and 2019 were examined for the prevalence of plasmid-mediated quinolone resistance (PMQR) in Escherichia coli. We detected qnrS-positive E. coli in a total of 74 samples from feces (n = 59), the carcass surface (n = 7), and retail meat (n = 8). These 74 qnrS-positive isolates were tested for antimicrobial susceptibility to determine the minimum inhibitory concentrations (MICs) of certain antimicrobials and genetically characterized. Ampicillin-resistance accounted for 71 of the 74 isolates (96%), followed by resistance to oxytetracycline (57/74; 77%), enrofloxacin (ERFX) (56/74; 76%), sulfisoxazole (SUL) (56/74; 76%), trimethoprim (TMP) (49/74; 66%), and dihydrostreptomycin (48/74; 65%). All farm-borne SUL- and TMP-resistant isolates except one were obtained from samples from farm A where a combination of sulfadiazine and TMP was administered to the chickens. Concentrations of ERFX at which 50 and 90% of isolates were inhibited were 2 μg/mL and 32 μg/mL, respectively. Diverse pulsed-field gel electrophoresis (PFGE) patterns of XbaI-digested genomic DNA were observed in the qnrS-positive isolates from fecal samples. Several isolates from feces and the carcass surface had identical XbaI-digested PFGE patterns. S1-nuclease PFGE and Southern blot analysis demonstrated that 7 of 11 dfrA13-positive fecal isolates carried both the qnrS and dfrA13 genes on the same plasmid, and 2 of 3 dfrA1-positive isolates similarly carried both qnrS and dfrA1 on the same plasmid, although the PFGE patterns of XbaI-digested genomic DNA of the isolates were different. These results suggest that the qnrS gene is prevalent in chicken farms via horizontal transfer of plasmids and may partly be co-selected under the use of TMP. |
| first_indexed | 2024-12-24T11:18:26Z |
| format | Article |
| id | doaj.art-1eec35125749487f9ad331b7e3c59d73 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-24T11:18:26Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj.art-1eec35125749487f9ad331b7e3c59d732022-12-21T16:58:18ZengElsevierPoultry Science0032-57912022-01-011011101538Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprimToshiyuki Murase0Patchara Phuektes1Hiroichi Ozaki2Sunpetch Angkititrakul3Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan; The Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan; Corresponding author:Faculty of Veterinary Medicine, Khon Kaen University, 40002, Thailand; Research Group of Emerging and Reemerging Infectious Diseases in Animals and Zoonotic Diseases, Faculty of Veterinary Medicine, Khon Kaen University, 40002, ThailandLaboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan; The Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University, Tottori 680-8553, JapanFaculty of Veterinary Medicine, Khon Kaen University, 40002, ThailandABSTRACT: One hundred and twenty chicken samples from feces (n = 80), the carcass surface at slaughter at 2 meat chicken farms (n = 20), and retail chicken meat from 5 markets (n = 20) collected during 2018 and 2019 were examined for the prevalence of plasmid-mediated quinolone resistance (PMQR) in Escherichia coli. We detected qnrS-positive E. coli in a total of 74 samples from feces (n = 59), the carcass surface (n = 7), and retail meat (n = 8). These 74 qnrS-positive isolates were tested for antimicrobial susceptibility to determine the minimum inhibitory concentrations (MICs) of certain antimicrobials and genetically characterized. Ampicillin-resistance accounted for 71 of the 74 isolates (96%), followed by resistance to oxytetracycline (57/74; 77%), enrofloxacin (ERFX) (56/74; 76%), sulfisoxazole (SUL) (56/74; 76%), trimethoprim (TMP) (49/74; 66%), and dihydrostreptomycin (48/74; 65%). All farm-borne SUL- and TMP-resistant isolates except one were obtained from samples from farm A where a combination of sulfadiazine and TMP was administered to the chickens. Concentrations of ERFX at which 50 and 90% of isolates were inhibited were 2 μg/mL and 32 μg/mL, respectively. Diverse pulsed-field gel electrophoresis (PFGE) patterns of XbaI-digested genomic DNA were observed in the qnrS-positive isolates from fecal samples. Several isolates from feces and the carcass surface had identical XbaI-digested PFGE patterns. S1-nuclease PFGE and Southern blot analysis demonstrated that 7 of 11 dfrA13-positive fecal isolates carried both the qnrS and dfrA13 genes on the same plasmid, and 2 of 3 dfrA1-positive isolates similarly carried both qnrS and dfrA1 on the same plasmid, although the PFGE patterns of XbaI-digested genomic DNA of the isolates were different. These results suggest that the qnrS gene is prevalent in chicken farms via horizontal transfer of plasmids and may partly be co-selected under the use of TMP.http://www.sciencedirect.com/science/article/pii/S0032579121005605chickenEscherichia coliplasmid-mediated quinolone resistanceqnrStrimethoprim |
| spellingShingle | Toshiyuki Murase Patchara Phuektes Hiroichi Ozaki Sunpetch Angkititrakul Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim Poultry Science chicken Escherichia coli plasmid-mediated quinolone resistance qnrS trimethoprim |
| title | Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim |
| title_full | Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim |
| title_fullStr | Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim |
| title_full_unstemmed | Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim |
| title_short | Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim |
| title_sort | prevalence of qnrs positive escherichia coli from chicken in thailand and possible co selection of isolates with plasmids carrying qnrs and trimethoprim resistance genes under farm use of trimethoprim |
| topic | chicken Escherichia coli plasmid-mediated quinolone resistance qnrS trimethoprim |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121005605 |
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