Polymer-coated hexagonal upconverting nanoparticles: chemical stability and cytotoxicity
Large (120 nm) hexagonal NaYF4:Yb, Er nanoparticles (UCNPs) were synthesized by high-temperature coprecipitation method and coated with poly(ethylene glycol)-alendronate (PEG-Ale), poly (N,N-dimethylacrylamide-co-2-aminoethylacrylamide)-alendronate (PDMA-Ale) or poly(methyl vinyl ether-co-maleic aci...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1207984/full |
| _version_ | 1827918045381656576 |
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| author | Vitalii Patsula Dana Mareková Dana Mareková Pavla Jendelová Pavla Jendelová Mykhailo Nahorniak Oleksandr Shapoval Petr Matouš Viktoriia Oleksa Rafał Konefał Magda Vosmanská Lucia Machová-Urdziková Daniel Horák |
| author_facet | Vitalii Patsula Dana Mareková Dana Mareková Pavla Jendelová Pavla Jendelová Mykhailo Nahorniak Oleksandr Shapoval Petr Matouš Viktoriia Oleksa Rafał Konefał Magda Vosmanská Lucia Machová-Urdziková Daniel Horák |
| author_sort | Vitalii Patsula |
| collection | DOAJ |
| description | Large (120 nm) hexagonal NaYF4:Yb, Er nanoparticles (UCNPs) were synthesized by high-temperature coprecipitation method and coated with poly(ethylene glycol)-alendronate (PEG-Ale), poly (N,N-dimethylacrylamide-co-2-aminoethylacrylamide)-alendronate (PDMA-Ale) or poly(methyl vinyl ether-co-maleic acid) (PMVEMA). The colloidal stability of polymer-coated UCNPs in water, PBS and DMEM medium was investigated by dynamic light scattering; UCNP@PMVEMA particles showed the best stability in PBS. Dissolution of the particles in water, PBS, DMEM and artificial lysosomal fluid (ALF) determined by potentiometric measurements showed that all particles were relatively chemically stable in DMEM. The UCNP@Ale-PEG and UCNP@Ale-PDMA particles were the least soluble in water and ALF, while the UCNP@PMVEMA particles were the most chemically stable in PBS. Green fluorescence of FITC-Ale-modified UCNPs was observed inside the cells, demonstrating successful internalization of particles into cells. The highest uptake was observed for neat UCNPs, followed by UCNP@Ale-PDMA and UCNP@PMVEMA. Viability of C6 cells and rat mesenchymal stem cells (rMSCs) growing in the presence of UCNPs was monitored by Alamar Blue assay. Culturing with UCNPs for 24 h did not affect cell viability. Prolonged incubation with particles for 72 h reduced cell viability to 40%–85% depending on the type of coating and nanoparticle concentration. The greatest decrease in cell viability was observed in cells cultured with neat UCNPs and UCNP@PMVEMA particles. Thanks to high upconversion luminescence, high cellular uptake and low toxicity, PDMA-coated hexagonal UCNPs may find future applications in cancer therapy. |
| first_indexed | 2024-03-13T03:38:10Z |
| format | Article |
| id | doaj.art-1eef06ca73734636b6493f34b3f18741 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-03-13T03:38:10Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-1eef06ca73734636b6493f34b3f187412023-06-23T13:47:35ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-06-011110.3389/fchem.2023.12079841207984Polymer-coated hexagonal upconverting nanoparticles: chemical stability and cytotoxicityVitalii Patsula0Dana Mareková1Dana Mareková2Pavla Jendelová3Pavla Jendelová4Mykhailo Nahorniak5Oleksandr Shapoval6Petr Matouš7Viktoriia Oleksa8Rafał Konefał9Magda Vosmanská10Lucia Machová-Urdziková11Daniel Horák12Institute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, CzechiaInstitute of Experimental Medicine, Czech Academy of Sciences, Prague, CzechiaDepartment of Neurosciences, Second Faculty of Medicine, Charles University, Prague, CzechiaInstitute of Experimental Medicine, Czech Academy of Sciences, Prague, CzechiaDepartment of Neurosciences, Second Faculty of Medicine, Charles University, Prague, CzechiaInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, CzechiaInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, CzechiaCenter for Advanced Preclinical Imaging, First Faculty of Medicine, Charles University, Prague, CzechiaInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, CzechiaInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, CzechiaDepartment of Analytical Chemistry, University of Chemistry and Technology Prague, Prague, CzechiaInstitute of Experimental Medicine, Czech Academy of Sciences, Prague, CzechiaInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, CzechiaLarge (120 nm) hexagonal NaYF4:Yb, Er nanoparticles (UCNPs) were synthesized by high-temperature coprecipitation method and coated with poly(ethylene glycol)-alendronate (PEG-Ale), poly (N,N-dimethylacrylamide-co-2-aminoethylacrylamide)-alendronate (PDMA-Ale) or poly(methyl vinyl ether-co-maleic acid) (PMVEMA). The colloidal stability of polymer-coated UCNPs in water, PBS and DMEM medium was investigated by dynamic light scattering; UCNP@PMVEMA particles showed the best stability in PBS. Dissolution of the particles in water, PBS, DMEM and artificial lysosomal fluid (ALF) determined by potentiometric measurements showed that all particles were relatively chemically stable in DMEM. The UCNP@Ale-PEG and UCNP@Ale-PDMA particles were the least soluble in water and ALF, while the UCNP@PMVEMA particles were the most chemically stable in PBS. Green fluorescence of FITC-Ale-modified UCNPs was observed inside the cells, demonstrating successful internalization of particles into cells. The highest uptake was observed for neat UCNPs, followed by UCNP@Ale-PDMA and UCNP@PMVEMA. Viability of C6 cells and rat mesenchymal stem cells (rMSCs) growing in the presence of UCNPs was monitored by Alamar Blue assay. Culturing with UCNPs for 24 h did not affect cell viability. Prolonged incubation with particles for 72 h reduced cell viability to 40%–85% depending on the type of coating and nanoparticle concentration. The greatest decrease in cell viability was observed in cells cultured with neat UCNPs and UCNP@PMVEMA particles. Thanks to high upconversion luminescence, high cellular uptake and low toxicity, PDMA-coated hexagonal UCNPs may find future applications in cancer therapy.https://www.frontiersin.org/articles/10.3389/fchem.2023.1207984/fullluminescenceupconversionnanoparticleslanthanidesdissolutioncell viability |
| spellingShingle | Vitalii Patsula Dana Mareková Dana Mareková Pavla Jendelová Pavla Jendelová Mykhailo Nahorniak Oleksandr Shapoval Petr Matouš Viktoriia Oleksa Rafał Konefał Magda Vosmanská Lucia Machová-Urdziková Daniel Horák Polymer-coated hexagonal upconverting nanoparticles: chemical stability and cytotoxicity Frontiers in Chemistry luminescence upconversion nanoparticles lanthanides dissolution cell viability |
| title | Polymer-coated hexagonal upconverting nanoparticles: chemical stability and cytotoxicity |
| title_full | Polymer-coated hexagonal upconverting nanoparticles: chemical stability and cytotoxicity |
| title_fullStr | Polymer-coated hexagonal upconverting nanoparticles: chemical stability and cytotoxicity |
| title_full_unstemmed | Polymer-coated hexagonal upconverting nanoparticles: chemical stability and cytotoxicity |
| title_short | Polymer-coated hexagonal upconverting nanoparticles: chemical stability and cytotoxicity |
| title_sort | polymer coated hexagonal upconverting nanoparticles chemical stability and cytotoxicity |
| topic | luminescence upconversion nanoparticles lanthanides dissolution cell viability |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1207984/full |
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