<i>Lacticaseibacillus rhamnosus</i> MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced Rats
Periodontitis is a chronic inflammatory disease characterized by tooth loss due to inflammation and the loss of alveolar bone. Periodontitis is closely related to various systemic diseases and is emerging as a global health problem. In this study, we investigated the anti-inflammatory effect of lact...
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MDPI AG
2022-11-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/22/4869 |
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| author | Seonyoung Kim Ji Yeon Lee Jeong-Yong Park YongGyeong Kim Chang-Ho Kang |
| author_facet | Seonyoung Kim Ji Yeon Lee Jeong-Yong Park YongGyeong Kim Chang-Ho Kang |
| author_sort | Seonyoung Kim |
| collection | DOAJ |
| description | Periodontitis is a chronic inflammatory disease characterized by tooth loss due to inflammation and the loss of alveolar bone. Periodontitis is closely related to various systemic diseases and is emerging as a global health problem. In this study, we investigated the anti-inflammatory effect of lactic acid bacteria (LAB) in vitro on <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) LPS-activated RAW264.7 and human gingival fibroblasts-1 (HGF-1) cells and the anti-osteoclastogenic effect of LAB on RANKL-induced RAW264.7 cells. All LAB strains (<i>Lacticaseibacillus rhamnosus</i> MG4706, MG4709, and MG4711) inhibited nitric oxide (NO)/<i>inducible nitric oxide synthase</i> (<i>iNOS</i>) in <i>P. gingivalis</i> LPS-activated RAW264.7 cells and pro-inflammatory cytokines (<i>IL-1β</i> and <i>IL-6</i>) and matrix metalloproteinase (<i>MMP-8</i> and <i>MMP-9</i>) in HGF-1 cells. In addition, LAB treatment inhibited osteoclastogenesis by reducing tartrate-resistant acid phosphatase (TRAP) activity and <i>cathepsin K</i> (<i>CtsK</i>) through the downregulation of <i>nuclear factor of activated T cells cytoplasmic 1</i> (<i>NFATc1</i>) and <i>c-fos</i> gene expression in RANKL-induced RAW264.7 cells. Administration of MG4706 alleviated alveolar bone loss indices and reduced the gene expression of <i>IL-1β</i>, <i>IL-6</i>, <i>MMP-8, MMP-9,</i> and <i>RANKL</i>/<i>OPG</i> ratio in gingival tissue. In conclusion, <i>L. rhamnosus</i> MG4706 has the potential to alleviate periodontitis. |
| first_indexed | 2024-03-09T18:05:02Z |
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| language | English |
| last_indexed | 2024-03-09T18:05:02Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-1ef1107b799c46f5adff124dc17593982023-11-24T09:32:30ZengMDPI AGNutrients2072-66432022-11-011422486910.3390/nu14224869<i>Lacticaseibacillus rhamnosus</i> MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced RatsSeonyoung Kim0Ji Yeon Lee1Jeong-Yong Park2YongGyeong Kim3Chang-Ho Kang4MEDIOGEN Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, Republic of KoreaMEDIOGEN Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, Republic of KoreaMEDIOGEN Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, Republic of KoreaMEDIOGEN Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, Republic of KoreaMEDIOGEN Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, Republic of KoreaPeriodontitis is a chronic inflammatory disease characterized by tooth loss due to inflammation and the loss of alveolar bone. Periodontitis is closely related to various systemic diseases and is emerging as a global health problem. In this study, we investigated the anti-inflammatory effect of lactic acid bacteria (LAB) in vitro on <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) LPS-activated RAW264.7 and human gingival fibroblasts-1 (HGF-1) cells and the anti-osteoclastogenic effect of LAB on RANKL-induced RAW264.7 cells. All LAB strains (<i>Lacticaseibacillus rhamnosus</i> MG4706, MG4709, and MG4711) inhibited nitric oxide (NO)/<i>inducible nitric oxide synthase</i> (<i>iNOS</i>) in <i>P. gingivalis</i> LPS-activated RAW264.7 cells and pro-inflammatory cytokines (<i>IL-1β</i> and <i>IL-6</i>) and matrix metalloproteinase (<i>MMP-8</i> and <i>MMP-9</i>) in HGF-1 cells. In addition, LAB treatment inhibited osteoclastogenesis by reducing tartrate-resistant acid phosphatase (TRAP) activity and <i>cathepsin K</i> (<i>CtsK</i>) through the downregulation of <i>nuclear factor of activated T cells cytoplasmic 1</i> (<i>NFATc1</i>) and <i>c-fos</i> gene expression in RANKL-induced RAW264.7 cells. Administration of MG4706 alleviated alveolar bone loss indices and reduced the gene expression of <i>IL-1β</i>, <i>IL-6</i>, <i>MMP-8, MMP-9,</i> and <i>RANKL</i>/<i>OPG</i> ratio in gingival tissue. In conclusion, <i>L. rhamnosus</i> MG4706 has the potential to alleviate periodontitis.https://www.mdpi.com/2072-6643/14/22/4869lactic acid bacteriaanti-inflammatoryanti-osteoclastogenesisperiodontitisRANKL/OPG system |
| spellingShingle | Seonyoung Kim Ji Yeon Lee Jeong-Yong Park YongGyeong Kim Chang-Ho Kang <i>Lacticaseibacillus rhamnosus</i> MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced Rats Nutrients lactic acid bacteria anti-inflammatory anti-osteoclastogenesis periodontitis RANKL/OPG system |
| title | <i>Lacticaseibacillus rhamnosus</i> MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced Rats |
| title_full | <i>Lacticaseibacillus rhamnosus</i> MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced Rats |
| title_fullStr | <i>Lacticaseibacillus rhamnosus</i> MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced Rats |
| title_full_unstemmed | <i>Lacticaseibacillus rhamnosus</i> MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced Rats |
| title_short | <i>Lacticaseibacillus rhamnosus</i> MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced Rats |
| title_sort | i lacticaseibacillus rhamnosus i mg4706 suppresses periodontitis in osteoclasts inflammation inducing cells and ligature induced rats |
| topic | lactic acid bacteria anti-inflammatory anti-osteoclastogenesis periodontitis RANKL/OPG system |
| url | https://www.mdpi.com/2072-6643/14/22/4869 |
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