PTBP1 plays an important role in the development of gastric cancer
Abstract Background Polypyrimidine tract binding protein 1 (PTBP1) has been found to play an important role in the occurrence and development of various tumors. At present, the role of PTBP1 in gastric cancer (GC) is still unknown and worthy of further investigation. Methods We used bioinformatics t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-023-03043-0 |
| _version_ | 1797451388833234944 |
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| author | Zewen Chu Miao Zhu Yuanyuan Luo Yaqi Hu Xinyi Feng Haibo Wang Masataka Sunagawa Yanqing Liu |
| author_facet | Zewen Chu Miao Zhu Yuanyuan Luo Yaqi Hu Xinyi Feng Haibo Wang Masataka Sunagawa Yanqing Liu |
| author_sort | Zewen Chu |
| collection | DOAJ |
| description | Abstract Background Polypyrimidine tract binding protein 1 (PTBP1) has been found to play an important role in the occurrence and development of various tumors. At present, the role of PTBP1 in gastric cancer (GC) is still unknown and worthy of further investigation. Methods We used bioinformatics to analyze the expression of PTBP1 in patients with GC. Cell proliferation related experiments were used to detect cell proliferation after PTBP1 knockdown. Skeleton staining, scanning electron microscopy and transmission electron microscopy were used to observe the changes of actin skeleton. Proliferation and actin skeleton remodeling signaling pathways were detected by Western Blots. The relationship between PTBP1 and proliferation of gastric cancer cells was further detected by subcutaneous tumor transplantation. Finally, tissue microarray data from clinical samples were used to further explore the expression of PTBP1 in patients with gastric cancer and its correlation with prognosis. Results Through bioinformatics studies, we found that PTBP1 was highly expressed in GC patients and correlated with poor prognosis. Cell proliferation and cycle analysis showed that PTBP1 down-regulation could significantly inhibit cell proliferation. The results of cell proliferation detection related experiments showed that PTBP1 down-regulation could inhibit the division and proliferation of GC cells. Furthermore, changes in the morphology of the actin skeleton of cells showed that PTBP1 down-regulation inhibited actin skeletal remodeling in GC cells. Western Blots showed that PTBP1 could regulate proliferation and actin skeleton remodeling signaling pathways. In addition, we constructed PTBP1 Cas9-KO mouse model and performed xenograft assays to further confirm that down-regulation of PTBP1 could inhibit the proliferation of GC cells. Finally, tissue microarray was used to further verify the close correlation between PTBP1 and poor prognosis in patients with GC. Conclusions Our study demonstrates for the first time that PTBP1 may affect the proliferation of GC cells by regulating actin skeleton remodeling. In addition, PTBP1 is closely related to actin skeleton remodeling and proliferation signaling pathways. We suppose that PTBP1 might be a potential target for the treatment of GC. Graphical abstract |
| first_indexed | 2024-03-09T14:53:57Z |
| format | Article |
| id | doaj.art-1ef3cb770b64442d9eaad7256296ada7 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-03-09T14:53:57Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-1ef3cb770b64442d9eaad7256296ada72023-11-26T14:18:37ZengBMCCancer Cell International1475-28672023-09-0123111810.1186/s12935-023-03043-0PTBP1 plays an important role in the development of gastric cancerZewen Chu0Miao Zhu1Yuanyuan Luo2Yaqi Hu3Xinyi Feng4Haibo Wang5Masataka Sunagawa6Yanqing Liu7The Affiliated Hospital of Yangzhou University, Yangzhou UniversityThe Affiliated Hospital of Yangzhou University, Yangzhou UniversityInstitute of Translational Medicine, Medical College, Yangzhou UniversityInstitute of Translational Medicine, Medical College, Yangzhou UniversityInstitute of Translational Medicine, Medical College, Yangzhou UniversityThe Affiliated Hospital of Yangzhou University, Yangzhou UniversityDepartment of physiology, School of Medicine, Showa UniversityThe Affiliated Hospital of Yangzhou University, Yangzhou UniversityAbstract Background Polypyrimidine tract binding protein 1 (PTBP1) has been found to play an important role in the occurrence and development of various tumors. At present, the role of PTBP1 in gastric cancer (GC) is still unknown and worthy of further investigation. Methods We used bioinformatics to analyze the expression of PTBP1 in patients with GC. Cell proliferation related experiments were used to detect cell proliferation after PTBP1 knockdown. Skeleton staining, scanning electron microscopy and transmission electron microscopy were used to observe the changes of actin skeleton. Proliferation and actin skeleton remodeling signaling pathways were detected by Western Blots. The relationship between PTBP1 and proliferation of gastric cancer cells was further detected by subcutaneous tumor transplantation. Finally, tissue microarray data from clinical samples were used to further explore the expression of PTBP1 in patients with gastric cancer and its correlation with prognosis. Results Through bioinformatics studies, we found that PTBP1 was highly expressed in GC patients and correlated with poor prognosis. Cell proliferation and cycle analysis showed that PTBP1 down-regulation could significantly inhibit cell proliferation. The results of cell proliferation detection related experiments showed that PTBP1 down-regulation could inhibit the division and proliferation of GC cells. Furthermore, changes in the morphology of the actin skeleton of cells showed that PTBP1 down-regulation inhibited actin skeletal remodeling in GC cells. Western Blots showed that PTBP1 could regulate proliferation and actin skeleton remodeling signaling pathways. In addition, we constructed PTBP1 Cas9-KO mouse model and performed xenograft assays to further confirm that down-regulation of PTBP1 could inhibit the proliferation of GC cells. Finally, tissue microarray was used to further verify the close correlation between PTBP1 and poor prognosis in patients with GC. Conclusions Our study demonstrates for the first time that PTBP1 may affect the proliferation of GC cells by regulating actin skeleton remodeling. In addition, PTBP1 is closely related to actin skeleton remodeling and proliferation signaling pathways. We suppose that PTBP1 might be a potential target for the treatment of GC. Graphical abstracthttps://doi.org/10.1186/s12935-023-03043-0PTBP1:Polypyrimidine Tract binding protein 1Gastric cancerProliferationActin cytoskeleton remodelingPTBP1 Cas9-KO mouse model |
| spellingShingle | Zewen Chu Miao Zhu Yuanyuan Luo Yaqi Hu Xinyi Feng Haibo Wang Masataka Sunagawa Yanqing Liu PTBP1 plays an important role in the development of gastric cancer Cancer Cell International PTBP1:Polypyrimidine Tract binding protein 1 Gastric cancer Proliferation Actin cytoskeleton remodeling PTBP1 Cas9-KO mouse model |
| title | PTBP1 plays an important role in the development of gastric cancer |
| title_full | PTBP1 plays an important role in the development of gastric cancer |
| title_fullStr | PTBP1 plays an important role in the development of gastric cancer |
| title_full_unstemmed | PTBP1 plays an important role in the development of gastric cancer |
| title_short | PTBP1 plays an important role in the development of gastric cancer |
| title_sort | ptbp1 plays an important role in the development of gastric cancer |
| topic | PTBP1:Polypyrimidine Tract binding protein 1 Gastric cancer Proliferation Actin cytoskeleton remodeling PTBP1 Cas9-KO mouse model |
| url | https://doi.org/10.1186/s12935-023-03043-0 |
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