Plasma metabolite score correlates with Hypoxia time in a newly born piglet model for asphyxia
Hypoxic-ischemic encephalopathy (HIE) secondary to perinatal asphyxia is a leading cause of mortality and acquired long-term neurologic co-morbidities in the neonate. The most successful intervention for the treatment of moderate to severe HIE is moderate whole body hypothermia initiated within 6 h...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-08-01
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| Series: | Redox Biology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231717300332 |
| _version_ | 1818483850666311680 |
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| author | Julia Kuligowski Rønnaug Solberg Ángel Sánchez-Illana Leonid Pankratov Anna Parra-Llorca Guillermo Quintás Ola Didrik Saugstad Máximo Vento |
| author_facet | Julia Kuligowski Rønnaug Solberg Ángel Sánchez-Illana Leonid Pankratov Anna Parra-Llorca Guillermo Quintás Ola Didrik Saugstad Máximo Vento |
| author_sort | Julia Kuligowski |
| collection | DOAJ |
| description | Hypoxic-ischemic encephalopathy (HIE) secondary to perinatal asphyxia is a leading cause of mortality and acquired long-term neurologic co-morbidities in the neonate. The most successful intervention for the treatment of moderate to severe HIE is moderate whole body hypothermia initiated within 6 h from birth. The objective and prompt identification of infants who are at risk of developing moderate to severe HIE in the critical first hours still remains a challenge. This work proposes a metabolite score calculated based on the relative intensities of three metabolites (choline, 6,8-dihydroxypurine and hypoxanthine) that showed maximum correlation with hypoxia time in a consolidated piglet model for neonatal hypoxia-ischemia. The metabolite score's performance as a biomarker for perinatal hypoxia and its usefulness for clinical grading and decision making have been assessed and compared to the performance of lactate which is currently considered the gold standard. For plasma samples withdrawn before and directly after a hypoxic insult, the metabolite score performed similar to lactate. However, it provided an enhanced predictive capacity at 2 h after resuscitation. The present study evidences the usefulness of the metabolite score for improving the early assessment of the severity of the hypoxic insult based on serial determinations in a minimally invasive biofluid. The applicability of the metabolite score for clinical diagnosis and patient stratification for hypothermia treatment has to be confirmed in multicenter trials involving newborns suffering from HIE. Keywords: Hypoxia, Perinatal asphyxia, Newborn, Metabolic biomarker, Neonatal piglet model, Liquid Chromatography – Time-of-Flight Mass Spectrometry (LC-TOF-MS) |
| first_indexed | 2024-12-10T15:47:33Z |
| format | Article |
| id | doaj.art-1ef546cd996642178b63aec782928d09 |
| institution | Directory Open Access Journal |
| issn | 2213-2317 |
| language | English |
| last_indexed | 2024-12-10T15:47:33Z |
| publishDate | 2017-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj.art-1ef546cd996642178b63aec782928d092022-12-22T01:42:55ZengElsevierRedox Biology2213-23172017-08-011217Plasma metabolite score correlates with Hypoxia time in a newly born piglet model for asphyxiaJulia Kuligowski0Rønnaug Solberg1Ángel Sánchez-Illana2Leonid Pankratov3Anna Parra-Llorca4Guillermo Quintás5Ola Didrik Saugstad6Máximo Vento7Neonatal Research Group, Health Research Institute Hospital La Fe, Avenida Fernando Abril Martorell 106, Valencia, SpainDepartment of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Oslo, Norway; Department of Pediatrics, Vestfold Hospital Trust, Tønsberg, NorwayNeonatal Research Group, Health Research Institute Hospital La Fe, Avenida Fernando Abril Martorell 106, Valencia, SpainDepartment of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Oslo, NorwayNeonatal Research Group, Health Research Institute Hospital La Fe, Avenida Fernando Abril Martorell 106, Valencia, SpainHuman & Environmental Health & Safety (HEHS), Leitat Technological Center, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain; Unidad Analítica, Health Research Institute La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, SpainDepartment of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Oslo, NorwayNeonatal Research Group, Health Research Institute Hospital La Fe, Avenida Fernando Abril Martorell 106, Valencia, Spain; Division of Neonatology, University & Polytechnic Hospital La Fe, Avenida Fernando Abril Martorell 106, Valencia, Spain; Corresponding author at: Division of Neonatology, University & Polytechnic Hospital La Fe, Avenida Fernando Abril Martorell 106, Valencia, Spain.Hypoxic-ischemic encephalopathy (HIE) secondary to perinatal asphyxia is a leading cause of mortality and acquired long-term neurologic co-morbidities in the neonate. The most successful intervention for the treatment of moderate to severe HIE is moderate whole body hypothermia initiated within 6 h from birth. The objective and prompt identification of infants who are at risk of developing moderate to severe HIE in the critical first hours still remains a challenge. This work proposes a metabolite score calculated based on the relative intensities of three metabolites (choline, 6,8-dihydroxypurine and hypoxanthine) that showed maximum correlation with hypoxia time in a consolidated piglet model for neonatal hypoxia-ischemia. The metabolite score's performance as a biomarker for perinatal hypoxia and its usefulness for clinical grading and decision making have been assessed and compared to the performance of lactate which is currently considered the gold standard. For plasma samples withdrawn before and directly after a hypoxic insult, the metabolite score performed similar to lactate. However, it provided an enhanced predictive capacity at 2 h after resuscitation. The present study evidences the usefulness of the metabolite score for improving the early assessment of the severity of the hypoxic insult based on serial determinations in a minimally invasive biofluid. The applicability of the metabolite score for clinical diagnosis and patient stratification for hypothermia treatment has to be confirmed in multicenter trials involving newborns suffering from HIE. Keywords: Hypoxia, Perinatal asphyxia, Newborn, Metabolic biomarker, Neonatal piglet model, Liquid Chromatography – Time-of-Flight Mass Spectrometry (LC-TOF-MS)http://www.sciencedirect.com/science/article/pii/S2213231717300332 |
| spellingShingle | Julia Kuligowski Rønnaug Solberg Ángel Sánchez-Illana Leonid Pankratov Anna Parra-Llorca Guillermo Quintás Ola Didrik Saugstad Máximo Vento Plasma metabolite score correlates with Hypoxia time in a newly born piglet model for asphyxia Redox Biology |
| title | Plasma metabolite score correlates with Hypoxia time in a newly born piglet model for asphyxia |
| title_full | Plasma metabolite score correlates with Hypoxia time in a newly born piglet model for asphyxia |
| title_fullStr | Plasma metabolite score correlates with Hypoxia time in a newly born piglet model for asphyxia |
| title_full_unstemmed | Plasma metabolite score correlates with Hypoxia time in a newly born piglet model for asphyxia |
| title_short | Plasma metabolite score correlates with Hypoxia time in a newly born piglet model for asphyxia |
| title_sort | plasma metabolite score correlates with hypoxia time in a newly born piglet model for asphyxia |
| url | http://www.sciencedirect.com/science/article/pii/S2213231717300332 |
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