Surfactin production is not essential for pellicle and root-associated biofilm development of Bacillus subtilis
Secondary metabolites have an important impact on the biocontrol potential of soil-derived microbes. In addition, various microbe-produced chemicals have been suggested to impact the development and phenotypic differentiation of bacteria, including biofilms. The non-ribosomal synthesized lipopeptide...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Biofilm |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207520300034 |
| _version_ | 1818383899906015232 |
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| author | Maude Thérien Heiko T. Kiesewalter Emile Auria Vincent Charron-Lamoureux Mario Wibowo Gergely Maróti Ákos T. Kovács Pascale B. Beauregard |
| author_facet | Maude Thérien Heiko T. Kiesewalter Emile Auria Vincent Charron-Lamoureux Mario Wibowo Gergely Maróti Ákos T. Kovács Pascale B. Beauregard |
| author_sort | Maude Thérien |
| collection | DOAJ |
| description | Secondary metabolites have an important impact on the biocontrol potential of soil-derived microbes. In addition, various microbe-produced chemicals have been suggested to impact the development and phenotypic differentiation of bacteria, including biofilms. The non-ribosomal synthesized lipopeptide of Bacillus subtilis, surfactin, has been described to impact the plant promoting capacity of the bacterium. Here, we investigated the impact of surfactin production on biofilm formation of B. subtilis using the laboratory model systems; pellicle formation at the air-medium interface and architecturally complex colony development, in addition to plant root-associated biofilms. We found that the production of surfactin by B. subtilis is not essential for pellicle biofilm formation neither in the well-studied strain, NCIB 3610, nor in the newly isolated environmental strains, but lack of surfactin reduces colony expansion. Further, plant root colonization was comparable both in the presence or absence of surfactin synthesis. Our results suggest that surfactin-related biocontrol and plant promotion in B. subtilis strains are independent of biofilm formation. |
| first_indexed | 2024-12-14T03:13:42Z |
| format | Article |
| id | doaj.art-1ef7aca8753b4e33af05c764a1cd48f2 |
| institution | Directory Open Access Journal |
| issn | 2590-2075 |
| language | English |
| last_indexed | 2024-12-14T03:13:42Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj.art-1ef7aca8753b4e33af05c764a1cd48f22022-12-21T23:19:12ZengElsevierBiofilm2590-20752020-12-012100021Surfactin production is not essential for pellicle and root-associated biofilm development of Bacillus subtilisMaude Thérien0Heiko T. Kiesewalter1Emile Auria2Vincent Charron-Lamoureux3Mario Wibowo4Gergely Maróti5Ákos T. Kovács6Pascale B. Beauregard7Centre SÈVE, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, CanadaBacterial Interactions and Evolution Group, DTU Bioengineering, Technical University of Denmark, Kgs Lyngby, DenmarkCentre SÈVE, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Canada; Biology Department, Ecole Normale Supérieure Paris-Saclay, Paris-Saclay University, Cachan, FranceCentre SÈVE, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, CanadaNatural Product Discovery Group, DTU Bioengineering, Technical University of Denmark, Kgs Lyngby, DenmarkInstitute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, Szeged, HungaryBacterial Interactions and Evolution Group, DTU Bioengineering, Technical University of Denmark, Kgs Lyngby, Denmark; Corresponding author.Centre SÈVE, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Canada; Corresponding author.Secondary metabolites have an important impact on the biocontrol potential of soil-derived microbes. In addition, various microbe-produced chemicals have been suggested to impact the development and phenotypic differentiation of bacteria, including biofilms. The non-ribosomal synthesized lipopeptide of Bacillus subtilis, surfactin, has been described to impact the plant promoting capacity of the bacterium. Here, we investigated the impact of surfactin production on biofilm formation of B. subtilis using the laboratory model systems; pellicle formation at the air-medium interface and architecturally complex colony development, in addition to plant root-associated biofilms. We found that the production of surfactin by B. subtilis is not essential for pellicle biofilm formation neither in the well-studied strain, NCIB 3610, nor in the newly isolated environmental strains, but lack of surfactin reduces colony expansion. Further, plant root colonization was comparable both in the presence or absence of surfactin synthesis. Our results suggest that surfactin-related biocontrol and plant promotion in B. subtilis strains are independent of biofilm formation.http://www.sciencedirect.com/science/article/pii/S2590207520300034Bacillus subtilisBiofilmSurfactinPlant root colonizationPellicle |
| spellingShingle | Maude Thérien Heiko T. Kiesewalter Emile Auria Vincent Charron-Lamoureux Mario Wibowo Gergely Maróti Ákos T. Kovács Pascale B. Beauregard Surfactin production is not essential for pellicle and root-associated biofilm development of Bacillus subtilis Biofilm Bacillus subtilis Biofilm Surfactin Plant root colonization Pellicle |
| title | Surfactin production is not essential for pellicle and root-associated biofilm development of Bacillus subtilis |
| title_full | Surfactin production is not essential for pellicle and root-associated biofilm development of Bacillus subtilis |
| title_fullStr | Surfactin production is not essential for pellicle and root-associated biofilm development of Bacillus subtilis |
| title_full_unstemmed | Surfactin production is not essential for pellicle and root-associated biofilm development of Bacillus subtilis |
| title_short | Surfactin production is not essential for pellicle and root-associated biofilm development of Bacillus subtilis |
| title_sort | surfactin production is not essential for pellicle and root associated biofilm development of bacillus subtilis |
| topic | Bacillus subtilis Biofilm Surfactin Plant root colonization Pellicle |
| url | http://www.sciencedirect.com/science/article/pii/S2590207520300034 |
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