Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> Clonal Complex 398 Isolated from Pigs and Humans in Thailand
Livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) has been widespread globally in pigs and humans for decades. Nasal colonization of LA-MRSA is regarded as an occupational hazard to people who are regularly involved in livestock production. Our previous st...
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MDPI AG
2023-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/12/1745 |
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| author | Pawarut Narongpun Pattrarat Chanchaithong Junya Yamagishi Jeewan Thapa Chie Nakajima Yasuhiko Suzuki |
| author_facet | Pawarut Narongpun Pattrarat Chanchaithong Junya Yamagishi Jeewan Thapa Chie Nakajima Yasuhiko Suzuki |
| author_sort | Pawarut Narongpun |
| collection | DOAJ |
| description | Livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) has been widespread globally in pigs and humans for decades. Nasal colonization of LA-MRSA is regarded as an occupational hazard to people who are regularly involved in livestock production. Our previous study suggested pig-to-human transmission caused by LA-MRSA clonal complex (CC) 398, using traditional molecular typing methods. Instead, this study aimed to investigate the zoonotic transmission of LA-MRSA CC398 using whole genome sequencing (WGS) technologies. A total of 63 LA-MRSA isolates were identified and characterized in Thailand. Further, the 16 representatives of LA-MRSA CC9 and CC398, including porcine and worker isolates, were subjected to WGS on the Illumina Miseq platform. Core-genome single nucleotide polymorphism (SNP)-based analyses verify the zoonotic transmission caused by LA-MRSA CC398 in two farms. WGS-based characterization suggests the emergence of a novel staphylococcal cassette chromosome (SCC) <i>mec</i> type, consisting of multiple cassette chromosome recombinase (<i>ccr</i>) gene complexes via genetic recombination. Additionally, the WGS analyses revealed putative multi-resistant plasmids and several cross-resistance genes, conferring resistance against drugs of last resort used in humans such as quinupristin/dalfopristin and linezolid. Significantly, LA-MRSA isolates, in this study, harbored multiple virulence genes that may become a serious threat to an immunosuppressive population, particularly for persons who are in close contact with LA-MRSA carriers. |
| first_indexed | 2024-03-08T21:02:16Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-08T21:02:16Z |
| publishDate | 2023-12-01 |
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| series | Antibiotics |
| spelling | doaj.art-1ef81127e2e84017852a9a67b3d091d12023-12-22T13:48:07ZengMDPI AGAntibiotics2079-63822023-12-011212174510.3390/antibiotics12121745Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> Clonal Complex 398 Isolated from Pigs and Humans in ThailandPawarut Narongpun0Pattrarat Chanchaithong1Junya Yamagishi2Jeewan Thapa3Chie Nakajima4Yasuhiko Suzuki5Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo 001-0020, JapanDepartment of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDivision of Collaboration and Education, Hokkaido University International Institute for Zoonosis Control, Sapporo 001-0020, JapanDivision of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo 001-0020, JapanDivision of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo 001-0020, JapanDivision of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo 001-0020, JapanLivestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) has been widespread globally in pigs and humans for decades. Nasal colonization of LA-MRSA is regarded as an occupational hazard to people who are regularly involved in livestock production. Our previous study suggested pig-to-human transmission caused by LA-MRSA clonal complex (CC) 398, using traditional molecular typing methods. Instead, this study aimed to investigate the zoonotic transmission of LA-MRSA CC398 using whole genome sequencing (WGS) technologies. A total of 63 LA-MRSA isolates were identified and characterized in Thailand. Further, the 16 representatives of LA-MRSA CC9 and CC398, including porcine and worker isolates, were subjected to WGS on the Illumina Miseq platform. Core-genome single nucleotide polymorphism (SNP)-based analyses verify the zoonotic transmission caused by LA-MRSA CC398 in two farms. WGS-based characterization suggests the emergence of a novel staphylococcal cassette chromosome (SCC) <i>mec</i> type, consisting of multiple cassette chromosome recombinase (<i>ccr</i>) gene complexes via genetic recombination. Additionally, the WGS analyses revealed putative multi-resistant plasmids and several cross-resistance genes, conferring resistance against drugs of last resort used in humans such as quinupristin/dalfopristin and linezolid. Significantly, LA-MRSA isolates, in this study, harbored multiple virulence genes that may become a serious threat to an immunosuppressive population, particularly for persons who are in close contact with LA-MRSA carriers.https://www.mdpi.com/2079-6382/12/12/1745livestock-associated methicillin-resistant <i>Staphylococcus aureus</i>LA-MRSAantimicrobial resistancewhole-genome sequencingWGSpigs |
| spellingShingle | Pawarut Narongpun Pattrarat Chanchaithong Junya Yamagishi Jeewan Thapa Chie Nakajima Yasuhiko Suzuki Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> Clonal Complex 398 Isolated from Pigs and Humans in Thailand Antibiotics livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> LA-MRSA antimicrobial resistance whole-genome sequencing WGS pigs |
| title | Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> Clonal Complex 398 Isolated from Pigs and Humans in Thailand |
| title_full | Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> Clonal Complex 398 Isolated from Pigs and Humans in Thailand |
| title_fullStr | Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> Clonal Complex 398 Isolated from Pigs and Humans in Thailand |
| title_full_unstemmed | Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> Clonal Complex 398 Isolated from Pigs and Humans in Thailand |
| title_short | Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> Clonal Complex 398 Isolated from Pigs and Humans in Thailand |
| title_sort | whole genome investigation of zoonotic transmission of livestock associated methicillin resistant i staphylococcus aureus i clonal complex 398 isolated from pigs and humans in thailand |
| topic | livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> LA-MRSA antimicrobial resistance whole-genome sequencing WGS pigs |
| url | https://www.mdpi.com/2079-6382/12/12/1745 |
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