The predictive value of various indicators of sperm for male fertility
Introduction. DNA fragmentation of sperm is one of the possible causes of reduced fertility potential of men. However, a significant correlation between conventional semen parameters and sperm DNA fragmentation was not found. This fact determines the relevance of the study of the influence of variou...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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ABV-press
2016-03-01
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| Series: | Андрология и генитальная хирургия |
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| Online Access: | https://agx.abvpress.ru/jour/article/view/166 |
| _version_ | 1797883545439436800 |
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| author | A. Yu. Metelev A. B. Bogdanov E. V. Ivkin A. A. Mitrokhin M. M. Vodneva Ya. A. Cherkezov E. I. Veliev O. B. Loran |
| author_facet | A. Yu. Metelev A. B. Bogdanov E. V. Ivkin A. A. Mitrokhin M. M. Vodneva Ya. A. Cherkezov E. I. Veliev O. B. Loran |
| author_sort | A. Yu. Metelev |
| collection | DOAJ |
| description | Introduction. DNA fragmentation of sperm is one of the possible causes of reduced fertility potential of men. However, a significant correlation between conventional semen parameters and sperm DNA fragmentation was not found. This fact determines the relevance of the study of the influence of various parameters of sperm on male fertility.Materials and methods. The study included 60 men, aged 26–36 years (median – 30 years) with idiopathic infertility and the level of DNA fragmentation of sperm is higher than 15 %. These men were treated with hyperbaric oxygen therapy, after 3 months in vitro fertilization performed partners of these men. DNA fragmentation of sperm cells was determined by TUNEL (upper limit of normal – 15 %). The level of reactive oxygen species (ROS) of the ejaculate were determined by chemiluminescence (upper limit of normal – 0.64 mV/s).Results. The frequency of pregnancy in vitro fertilization was following: 62.8 and 64.7 % (p > 0.05) for the total number sperm of spermatozoa < 38 × 106 /ejaculate and ≥ 39 × 106 /ejaculate, respectively; 63.3 and 63.6 % (p > 0.05) for mobility (a + b) of spermatozoa < 40 and ≥ 40 %, respectively; 58.3 and 64.6 % (p > 0.05) for normal forms of spermatozoa < 4 and ≥ 4 %, respectively; 67.3 and 20.0 % (p < 0.05) for the level of DNA fragmentation of sperm ≤ 15 and > 15 %, respectively; 64.9 and 33.3 % (p < 0.05) for the level of ROS in semen ≤ 0.64 and > 0.64 mV/s, respectively.Conclusion. The probability of pregnancy after in vitro fertilization significantly depends on the levels of sperm DNA fragmentation in the sperm and level of ROS in semen. |
| first_indexed | 2024-04-10T03:53:31Z |
| format | Article |
| id | doaj.art-1efb9b48ab64479ca5fbec7837291043 |
| institution | Directory Open Access Journal |
| issn | 2070-9781 |
| language | Russian |
| last_indexed | 2024-04-10T03:53:31Z |
| publishDate | 2016-03-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Андрология и генитальная хирургия |
| spelling | doaj.art-1efb9b48ab64479ca5fbec78372910432023-03-13T07:15:08ZrusABV-pressАндрология и генитальная хирургия2070-97812016-03-01164515410.17650/2070-9781-2015-16-4-51-54158The predictive value of various indicators of sperm for male fertilityA. Yu. Metelev0A. B. Bogdanov1E. V. Ivkin2A. A. Mitrokhin3M. M. Vodneva4Ya. A. Cherkezov5E. I. Veliev6O. B. Loran7S.P. Botkin Clinical Hospital, Moscow Healthcare DepartmentS.P. Botkin Clinical Hospital, Moscow Healthcare Department; Department of Urology and Surgical Andrology, Russian Medical Academy for Postgraduate Education, Ministry of Health of RussiaS.P. Botkin Clinical Hospital, Moscow Healthcare Department; Department of Urology and Surgical Andrology, Russian Medical Academy for Postgraduate Education, Ministry of Health of RussiaS.P. Botkin Clinical Hospital, Moscow Healthcare DepartmentS.P. Botkin Clinical Hospital, Moscow Healthcare DepartmentMoscow Regional Research Institute of Obstetrics and GynecologyS.P. Botkin Clinical Hospital, Moscow Healthcare Department; Department of Urology and Surgical Andrology, Russian Medical Academy for Postgraduate Education, Ministry of Health of RussiaS.P. Botkin Clinical Hospital, Moscow Healthcare Department; Department of Urology and Surgical Andrology, Russian Medical Academy for Postgraduate Education, Ministry of Health of RussiaIntroduction. DNA fragmentation of sperm is one of the possible causes of reduced fertility potential of men. However, a significant correlation between conventional semen parameters and sperm DNA fragmentation was not found. This fact determines the relevance of the study of the influence of various parameters of sperm on male fertility.Materials and methods. The study included 60 men, aged 26–36 years (median – 30 years) with idiopathic infertility and the level of DNA fragmentation of sperm is higher than 15 %. These men were treated with hyperbaric oxygen therapy, after 3 months in vitro fertilization performed partners of these men. DNA fragmentation of sperm cells was determined by TUNEL (upper limit of normal – 15 %). The level of reactive oxygen species (ROS) of the ejaculate were determined by chemiluminescence (upper limit of normal – 0.64 mV/s).Results. The frequency of pregnancy in vitro fertilization was following: 62.8 and 64.7 % (p > 0.05) for the total number sperm of spermatozoa < 38 × 106 /ejaculate and ≥ 39 × 106 /ejaculate, respectively; 63.3 and 63.6 % (p > 0.05) for mobility (a + b) of spermatozoa < 40 and ≥ 40 %, respectively; 58.3 and 64.6 % (p > 0.05) for normal forms of spermatozoa < 4 and ≥ 4 %, respectively; 67.3 and 20.0 % (p < 0.05) for the level of DNA fragmentation of sperm ≤ 15 and > 15 %, respectively; 64.9 and 33.3 % (p < 0.05) for the level of ROS in semen ≤ 0.64 and > 0.64 mV/s, respectively.Conclusion. The probability of pregnancy after in vitro fertilization significantly depends on the levels of sperm DNA fragmentation in the sperm and level of ROS in semen.https://agx.abvpress.ru/jour/article/view/166male infertilitysperm dna fragmentationreactive oxygen species in semen |
| spellingShingle | A. Yu. Metelev A. B. Bogdanov E. V. Ivkin A. A. Mitrokhin M. M. Vodneva Ya. A. Cherkezov E. I. Veliev O. B. Loran The predictive value of various indicators of sperm for male fertility Андрология и генитальная хирургия male infertility sperm dna fragmentation reactive oxygen species in semen |
| title | The predictive value of various indicators of sperm for male fertility |
| title_full | The predictive value of various indicators of sperm for male fertility |
| title_fullStr | The predictive value of various indicators of sperm for male fertility |
| title_full_unstemmed | The predictive value of various indicators of sperm for male fertility |
| title_short | The predictive value of various indicators of sperm for male fertility |
| title_sort | predictive value of various indicators of sperm for male fertility |
| topic | male infertility sperm dna fragmentation reactive oxygen species in semen |
| url | https://agx.abvpress.ru/jour/article/view/166 |
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