An extensively drug-resistant Pseudomonas aeruginosa isolate from Myanmar
ABSTRACT: Objectives: Antimicrobial resistance (AMR) including multidrug-resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa has emerged as one of the serious public health threats across the globe. Southeast Asia is a ‘hot spot’ of antimicrobial-resistant bacteria, includin...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716523001996 |
| _version_ | 1827311783234240512 |
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| author | Thanda Tun Sakiusa Cabe Baleivanualala Mareike Britta Erdmann Gregory Gimenez Htin Lin Aung |
| author_facet | Thanda Tun Sakiusa Cabe Baleivanualala Mareike Britta Erdmann Gregory Gimenez Htin Lin Aung |
| author_sort | Thanda Tun |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Antimicrobial resistance (AMR) including multidrug-resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa has emerged as one of the serious public health threats across the globe. Southeast Asia is a ‘hot spot’ of antimicrobial-resistant bacteria, including MDR P. aeruginosa. Despite Myanmar being located in Southeast Asia and suffering from a high infectious disease burden, data on MDR and XDR P. aeruginosa from Myanmar are limited. In this communication, we report the draft genome of an XDR P. aeruginosa isolate, MMXDRPA001, that was identified during a routine diagnosis in Myanmar. Methods: An MMXDRPA001 isolate colonising a hospitalised patient was characterised by antibiotic resistance profiling following standard methods and whole-genome sequencing using an Illumina MiSeq platform. The generated reads were de novo assembled using SPAdes (v.3.9.1). Annotation was performed by Prokka (v.1.14.0). Sequence type, antimicrobial resistance and virulence-related genes were predicted from the sequence. The phylogenetic relationships of all P. aeruginosa isolates were determined using core genome single-nucleotide polymorphisms (SNPs) analysis utilising Snippy (v.4.6.0) and Gubbins (v.2.3.4). Results: P. aeruginosa MMXDRPA001 was resistant to most antipseudomonal β-lactams, aminoglycosides and quinolones. The assembly comprised 145 contigs totalling 6 808 493 bases of sequence and a total of 6183 coding sequences. The isolate belonged to sequence type (ST) 235, contained carbapenemase-encoding gene blaIMP-1 and was clonally related to a previously reported isolate from Thailand. Conclusion: The identification of an international high-risk clone of ST235 XDR isolate in Myanmar, genomically relating to that from a neighbouring country underscores the need for coordinated AMR surveillance throughout healthcare settings in Myanmar and in the Southeast Asia region. |
| first_indexed | 2024-03-08T22:31:03Z |
| format | Article |
| id | doaj.art-1efc172fbe9e4aad9fed2c5027d41410 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-24T20:25:35Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-1efc172fbe9e4aad9fed2c5027d414102024-03-22T05:39:21ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-03-013613An extensively drug-resistant Pseudomonas aeruginosa isolate from MyanmarThanda Tun0Sakiusa Cabe Baleivanualala1Mareike Britta Erdmann2Gregory Gimenez3Htin Lin Aung4Yangon Central Women's Hospital, Yangon, Myanmar; West Yangon General Hospital, Yangon, MyanmarDepartment of Microbiology and Immunology, School of Biomedical Sciences, University of Otago, Dunedin, New ZealandDepartment of Microbiology and Immunology, School of Biomedical Sciences, University of Otago, Dunedin, New ZealandDepartment of Pathology, University of Otago, Dunedin, New ZealandDepartment of Microbiology and Immunology, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand; Corresponding author. Mailing address: Department of Microbiology and Immunology, School of Biomedical Sciences, University of Otago, P.O. Box 56, Dunedin, New Zealand.ABSTRACT: Objectives: Antimicrobial resistance (AMR) including multidrug-resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa has emerged as one of the serious public health threats across the globe. Southeast Asia is a ‘hot spot’ of antimicrobial-resistant bacteria, including MDR P. aeruginosa. Despite Myanmar being located in Southeast Asia and suffering from a high infectious disease burden, data on MDR and XDR P. aeruginosa from Myanmar are limited. In this communication, we report the draft genome of an XDR P. aeruginosa isolate, MMXDRPA001, that was identified during a routine diagnosis in Myanmar. Methods: An MMXDRPA001 isolate colonising a hospitalised patient was characterised by antibiotic resistance profiling following standard methods and whole-genome sequencing using an Illumina MiSeq platform. The generated reads were de novo assembled using SPAdes (v.3.9.1). Annotation was performed by Prokka (v.1.14.0). Sequence type, antimicrobial resistance and virulence-related genes were predicted from the sequence. The phylogenetic relationships of all P. aeruginosa isolates were determined using core genome single-nucleotide polymorphisms (SNPs) analysis utilising Snippy (v.4.6.0) and Gubbins (v.2.3.4). Results: P. aeruginosa MMXDRPA001 was resistant to most antipseudomonal β-lactams, aminoglycosides and quinolones. The assembly comprised 145 contigs totalling 6 808 493 bases of sequence and a total of 6183 coding sequences. The isolate belonged to sequence type (ST) 235, contained carbapenemase-encoding gene blaIMP-1 and was clonally related to a previously reported isolate from Thailand. Conclusion: The identification of an international high-risk clone of ST235 XDR isolate in Myanmar, genomically relating to that from a neighbouring country underscores the need for coordinated AMR surveillance throughout healthcare settings in Myanmar and in the Southeast Asia region.http://www.sciencedirect.com/science/article/pii/S2213716523001996CarbapenemsP. aeruginosaIMP metallo-β-lactamase |
| spellingShingle | Thanda Tun Sakiusa Cabe Baleivanualala Mareike Britta Erdmann Gregory Gimenez Htin Lin Aung An extensively drug-resistant Pseudomonas aeruginosa isolate from Myanmar Journal of Global Antimicrobial Resistance Carbapenems P. aeruginosa IMP metallo-β-lactamase |
| title | An extensively drug-resistant Pseudomonas aeruginosa isolate from Myanmar |
| title_full | An extensively drug-resistant Pseudomonas aeruginosa isolate from Myanmar |
| title_fullStr | An extensively drug-resistant Pseudomonas aeruginosa isolate from Myanmar |
| title_full_unstemmed | An extensively drug-resistant Pseudomonas aeruginosa isolate from Myanmar |
| title_short | An extensively drug-resistant Pseudomonas aeruginosa isolate from Myanmar |
| title_sort | extensively drug resistant pseudomonas aeruginosa isolate from myanmar |
| topic | Carbapenems P. aeruginosa IMP metallo-β-lactamase |
| url | http://www.sciencedirect.com/science/article/pii/S2213716523001996 |
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