Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage Analyses

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has frequently been applied to the analysis of biomolecules. Its strength resides not only in compound identification but particularly in acquiring molecular profiles providing a high discriminating power. Th...

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Main Author: Marek Šebela
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/23/21/13631
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author Marek Šebela
author_facet Marek Šebela
author_sort Marek Šebela
collection DOAJ
description Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has frequently been applied to the analysis of biomolecules. Its strength resides not only in compound identification but particularly in acquiring molecular profiles providing a high discriminating power. The main advantages include its speed, simplicity, versatility, minimum sample preparation needs, and a relatively high tolerance to salts. Other benefits are represented by the possibility of automation, high throughput, sensitivity, accuracy, and good reproducibility, allowing quantitative studies. This review deals with the prominent use of MALDI-TOF MS profiling in food and beverage analysis ranging from the simple detection of sample constituents to quantifications of marker compounds, quality control, and assessment of product authenticity. This review summarizes relevant discoveries that have been obtained with milk and milk products, edible oils, wine, beer, flour, meat, honey, and other alimentary products. Marker molecules are specified: proteins and peptides for milk, cheeses, flour, meat, wine and beer; triacylglycerols and phospholipids for oils; and low-molecular-weight metabolites for wine, beer and chocolate. Special attention is paid to sample preparation techniques and the combination of spectral profiling and statistical evaluation methods, which is powerful for the differentiation of samples and the sensitive detection of frauds and adulterations.
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spelling doaj.art-1efccc5c60354ae88a7b0469fdee05622023-11-24T05:11:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211363110.3390/ijms232113631Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage AnalysesMarek Šebela0Department of Biochemistry, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech RepublicMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has frequently been applied to the analysis of biomolecules. Its strength resides not only in compound identification but particularly in acquiring molecular profiles providing a high discriminating power. The main advantages include its speed, simplicity, versatility, minimum sample preparation needs, and a relatively high tolerance to salts. Other benefits are represented by the possibility of automation, high throughput, sensitivity, accuracy, and good reproducibility, allowing quantitative studies. This review deals with the prominent use of MALDI-TOF MS profiling in food and beverage analysis ranging from the simple detection of sample constituents to quantifications of marker compounds, quality control, and assessment of product authenticity. This review summarizes relevant discoveries that have been obtained with milk and milk products, edible oils, wine, beer, flour, meat, honey, and other alimentary products. Marker molecules are specified: proteins and peptides for milk, cheeses, flour, meat, wine and beer; triacylglycerols and phospholipids for oils; and low-molecular-weight metabolites for wine, beer and chocolate. Special attention is paid to sample preparation techniques and the combination of spectral profiling and statistical evaluation methods, which is powerful for the differentiation of samples and the sensitive detection of frauds and adulterations.https://www.mdpi.com/1422-0067/23/21/13631adulterationclassificationdifferentiationfoodmass spectrometryMALDI
spellingShingle Marek Šebela
Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage Analyses
International Journal of Molecular Sciences
adulteration
classification
differentiation
food
mass spectrometry
MALDI
title Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage Analyses
title_full Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage Analyses
title_fullStr Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage Analyses
title_full_unstemmed Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage Analyses
title_short Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage Analyses
title_sort biomolecular profiling by maldi tof mass spectrometry in food and beverage analyses
topic adulteration
classification
differentiation
food
mass spectrometry
MALDI
url https://www.mdpi.com/1422-0067/23/21/13631
work_keys_str_mv AT mareksebela biomolecularprofilingbymalditofmassspectrometryinfoodandbeverageanalyses