Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy
Abstract In classical bovine spongiform encephalopathy (C-BSE), an orally acquired prion disease of cattle, the ileal Peyer’s patch (IPP) represents the main entry port for the BSE agent. In earlier C-BSE pathogenesis studies, cattle at 4–6 months of age were orally challenged, while there are stron...
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-12-01
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| Series: | Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s13567-017-0495-5 |
| _version_ | 1818955549747707904 |
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| author | Ivett Ackermann Anne Balkema-Buschmann Reiner Ulrich Kerstin Tauscher James C. Shawulu Markus Keller Olanrewaju I. Fatola Paul Brown Martin H. Groschup |
| author_facet | Ivett Ackermann Anne Balkema-Buschmann Reiner Ulrich Kerstin Tauscher James C. Shawulu Markus Keller Olanrewaju I. Fatola Paul Brown Martin H. Groschup |
| author_sort | Ivett Ackermann |
| collection | DOAJ |
| description | Abstract In classical bovine spongiform encephalopathy (C-BSE), an orally acquired prion disease of cattle, the ileal Peyer’s patch (IPP) represents the main entry port for the BSE agent. In earlier C-BSE pathogenesis studies, cattle at 4–6 months of age were orally challenged, while there are strong indications that the risk of infection is highest in young animals. In the present study, unweaned calves aged 4–6 weeks were orally challenged to determine the earliest time point at which newly formed PrPBSE and BSE infectivity are detectable in the IPP. For this purpose, calves were culled 1 week as well as 2, 4, 6 and 8 months post-infection (mpi) and IPPs were examined for BSE infectivity using a bovine PrP transgenic mouse bioassay, and for PrPBSE by immunohistochemistry (IHC) and protein misfolding cyclic amplification (PMCA) assays. For the first time, BSE prions were detected in the IPP as early as 2 mpi by transgenic mouse bioassay and PMCA and 4 mpi by IHC in the follicular dendritic cells (FDCs) of the IPP follicles. These data indicate that BSE prions propagate in the IPP of unweaned calves within 2 months of oral uptake of the agent. |
| first_indexed | 2024-12-20T10:39:50Z |
| format | Article |
| id | doaj.art-1efe9ed09b124f1fbf2c4aabf43e4bd2 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-20T10:39:50Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-1efe9ed09b124f1fbf2c4aabf43e4bd22022-12-21T19:43:33ZengBMCVeterinary Research1297-97162017-12-0148111210.1186/s13567-017-0495-5Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathyIvett Ackermann0Anne Balkema-Buschmann1Reiner Ulrich2Kerstin Tauscher3James C. Shawulu4Markus Keller5Olanrewaju I. Fatola6Paul Brown7Martin H. Groschup8Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-InstitutInstitute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-InstitutDepartment of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-InstitutDepartment of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-InstitutInstitute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-InstitutInstitute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-InstitutInstitute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-InstitutNational Institute of Neurological Diseases and Stroke, National Institutes of HealthInstitute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-InstitutAbstract In classical bovine spongiform encephalopathy (C-BSE), an orally acquired prion disease of cattle, the ileal Peyer’s patch (IPP) represents the main entry port for the BSE agent. In earlier C-BSE pathogenesis studies, cattle at 4–6 months of age were orally challenged, while there are strong indications that the risk of infection is highest in young animals. In the present study, unweaned calves aged 4–6 weeks were orally challenged to determine the earliest time point at which newly formed PrPBSE and BSE infectivity are detectable in the IPP. For this purpose, calves were culled 1 week as well as 2, 4, 6 and 8 months post-infection (mpi) and IPPs were examined for BSE infectivity using a bovine PrP transgenic mouse bioassay, and for PrPBSE by immunohistochemistry (IHC) and protein misfolding cyclic amplification (PMCA) assays. For the first time, BSE prions were detected in the IPP as early as 2 mpi by transgenic mouse bioassay and PMCA and 4 mpi by IHC in the follicular dendritic cells (FDCs) of the IPP follicles. These data indicate that BSE prions propagate in the IPP of unweaned calves within 2 months of oral uptake of the agent.http://link.springer.com/article/10.1186/s13567-017-0495-5 |
| spellingShingle | Ivett Ackermann Anne Balkema-Buschmann Reiner Ulrich Kerstin Tauscher James C. Shawulu Markus Keller Olanrewaju I. Fatola Paul Brown Martin H. Groschup Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy Veterinary Research |
| title | Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy |
| title_full | Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy |
| title_fullStr | Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy |
| title_full_unstemmed | Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy |
| title_short | Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy |
| title_sort | detection of prpbse and prion infectivity in the ileal peyer s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy |
| url | http://link.springer.com/article/10.1186/s13567-017-0495-5 |
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