Down-regulated miR-9 and miR-433 in human gastric carcinoma
<p>Abstract</p> <p>Background</p> <p>MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.</p> <p>Methods</p> <p>To screening the special miRNAs in...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-06-01
|
| Series: | Journal of Experimental & Clinical Cancer Research |
| Online Access: | http://www.jeccr.com/content/28/1/82 |
| _version_ | 1818491406583332864 |
|---|---|
| author | Nie Na Guo Jinjun Ning Bo Zhang Xia Zhang Zhenzhen Zhang Hongbin Luo Hongchun Liu Bo Wu Xiaoling |
| author_facet | Nie Na Guo Jinjun Ning Bo Zhang Xia Zhang Zhenzhen Zhang Hongbin Luo Hongchun Liu Bo Wu Xiaoling |
| author_sort | Nie Na |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.</p> <p>Methods</p> <p>To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot.</p> <p>Results</p> <p>We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively.</p> <p>Conclusion</p> <p>Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.</p> |
| first_indexed | 2024-12-10T17:30:25Z |
| format | Article |
| id | doaj.art-1f00de0c821e445c82e1b4398fcb7638 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-10T17:30:25Z |
| publishDate | 2009-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-1f00de0c821e445c82e1b4398fcb76382022-12-22T01:39:42ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662009-06-012818210.1186/1756-9966-28-82Down-regulated miR-9 and miR-433 in human gastric carcinomaNie NaGuo JinjunNing BoZhang XiaZhang ZhenzhenZhang HongbinLuo HongchunLiu BoWu Xiaoling<p>Abstract</p> <p>Background</p> <p>MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.</p> <p>Methods</p> <p>To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot.</p> <p>Results</p> <p>We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively.</p> <p>Conclusion</p> <p>Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.</p>http://www.jeccr.com/content/28/1/82 |
| spellingShingle | Nie Na Guo Jinjun Ning Bo Zhang Xia Zhang Zhenzhen Zhang Hongbin Luo Hongchun Liu Bo Wu Xiaoling Down-regulated miR-9 and miR-433 in human gastric carcinoma Journal of Experimental & Clinical Cancer Research |
| title | Down-regulated miR-9 and miR-433 in human gastric carcinoma |
| title_full | Down-regulated miR-9 and miR-433 in human gastric carcinoma |
| title_fullStr | Down-regulated miR-9 and miR-433 in human gastric carcinoma |
| title_full_unstemmed | Down-regulated miR-9 and miR-433 in human gastric carcinoma |
| title_short | Down-regulated miR-9 and miR-433 in human gastric carcinoma |
| title_sort | down regulated mir 9 and mir 433 in human gastric carcinoma |
| url | http://www.jeccr.com/content/28/1/82 |
| work_keys_str_mv | AT niena downregulatedmir9andmir433inhumangastriccarcinoma AT guojinjun downregulatedmir9andmir433inhumangastriccarcinoma AT ningbo downregulatedmir9andmir433inhumangastriccarcinoma AT zhangxia downregulatedmir9andmir433inhumangastriccarcinoma AT zhangzhenzhen downregulatedmir9andmir433inhumangastriccarcinoma AT zhanghongbin downregulatedmir9andmir433inhumangastriccarcinoma AT luohongchun downregulatedmir9andmir433inhumangastriccarcinoma AT liubo downregulatedmir9andmir433inhumangastriccarcinoma AT wuxiaoling downregulatedmir9andmir433inhumangastriccarcinoma |