A robust method to isolate Drosophila fat body nuclei for transcriptomic analysis
Gene expression profiles are typically described at the level of the tissue or, often in Drosophila, at the level of the whole organism. Collapsing the gene expression of entire tissues into single measures averages over potentially important heterogeneity among the cells that make up that tissue. T...
Main Authors: | Vanika Gupta, Brian P. Lazzaro |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2022-12-01
|
Series: | Fly |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/19336934.2021.1978776 |
Similar Items
-
Small mosquitoes, large implications: crowding and starvation affects gene expression and nutrient accumulation in Aedes aegypti
by: David P Price, et al.
Published: (2015-04-01) -
Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling
by: Katie L. Whytock, et al.
Published: (2023-03-01) -
Isolation and RNA sequencing of single nuclei from Drosophila tissues
by: Colleen N. McLaughlin, et al.
Published: (2022-06-01) -
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
by: Cristina Soares de Sousa, et al.
Published: (2013-01-01) -
Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material
by: Gordon Wiegleb, et al.
Published: (2022-11-01)