Potential Probiotic Properties of Exopolysaccharide-Producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide
Exopolysaccharide (EPS)-producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS was isolated from healthy human feces and its probiotic properties, as well as the structure and prebiotic activity of the EPS from this strain were examined. EPS from <i>L. paracasei</i> EPS DA...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/12/2431 |
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| author | Min-Gyu Lee Huijin Joeng Jaein Shin Suin Kim Chaeeun Lee Youngbo Song Byung-Hoo Lee Hyoung-Geun Park Tae-Ho Lee Hai-Hua Jiang Young-Sun Han Bong-Gyeong Lee Ho-Jin Lee Min-Ju Park Yun-Ju Jun Young-Seo Park |
| author_facet | Min-Gyu Lee Huijin Joeng Jaein Shin Suin Kim Chaeeun Lee Youngbo Song Byung-Hoo Lee Hyoung-Geun Park Tae-Ho Lee Hai-Hua Jiang Young-Sun Han Bong-Gyeong Lee Ho-Jin Lee Min-Ju Park Yun-Ju Jun Young-Seo Park |
| author_sort | Min-Gyu Lee |
| collection | DOAJ |
| description | Exopolysaccharide (EPS)-producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS was isolated from healthy human feces and its probiotic properties, as well as the structure and prebiotic activity of the EPS from this strain were examined. EPS from <i>L. paracasei</i> EPS DA-BACS had a ropy phenotype, which is known to have potential health benefits and is identified as loosely cell-bounded glucomannan-type EPS with a molecular size of 3.7 × 10<sup>6</sup> Da. EPS promoted the acid tolerance of <i>L. paracasei</i> EPS DA-BACS and provided cells with tolerance to gastrointestinal stress. The purified EPS showed growth inhibitory activity against <i>Clostridium difficile. L. paracasei</i> EPS DA-BACS cells completely inhibited the growth of <i>Bacillus subtilis</i>, <i>Pseudomonas aeruginosa</i>, and <i>Aspergillus brasiliensis</i>, as well as showed high growth inhibitory activity against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. Treatment of lipopolysaccharide-stimulated RAW 264.7 cells with heat-killed <i>L. paracasei</i> EPS DA-BACS cells led to a decrease in the production of nitric oxide, indicating the anti-inflammatory activity of <i>L. paracasei</i> EPS DA-BACS. Purified EPS promoted the growth of <i>Lactobacillus gasseri, Bifidobacterium bifidum, B. animalis</i>, and <i>B. faecale</i> which showed high prebiotic activity. <i>L. paracasei</i> EPS DA-BACS harbors no antibiotic resistance genes or virulence factors. Therefore, <i>L. paracasei</i> EPS DA-BACS exhibits anti-inflammatory and antimicrobial activities with high gut adhesion ability and gastrointestinal tolerance and can be used as a potential probiotic. |
| first_indexed | 2024-03-09T16:03:57Z |
| format | Article |
| id | doaj.art-1f038c444d8645848765fce2389d5a47 |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:03:57Z |
| publishDate | 2022-12-01 |
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| series | Microorganisms |
| spelling | doaj.art-1f038c444d8645848765fce2389d5a472023-11-24T16:48:59ZengMDPI AGMicroorganisms2076-26072022-12-011012243110.3390/microorganisms10122431Potential Probiotic Properties of Exopolysaccharide-Producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS and Prebiotic Activity of Its ExopolysaccharideMin-Gyu Lee0Huijin Joeng1Jaein Shin2Suin Kim3Chaeeun Lee4Youngbo Song5Byung-Hoo Lee6Hyoung-Geun Park7Tae-Ho Lee8Hai-Hua Jiang9Young-Sun Han10Bong-Gyeong Lee11Ho-Jin Lee12Min-Ju Park13Yun-Ju Jun14Young-Seo Park15Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of KoreaResearch Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin 17073, Republic of KoreaResearch Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin 17073, Republic of KoreaResearch Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin 17073, Republic of KoreaResearch Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin 17073, Republic of KoreaResearch Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin 17073, Republic of KoreaResearch Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin 17073, Republic of KoreaResearch Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin 17073, Republic of KoreaResearch Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin 17073, Republic of KoreaDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of KoreaExopolysaccharide (EPS)-producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS was isolated from healthy human feces and its probiotic properties, as well as the structure and prebiotic activity of the EPS from this strain were examined. EPS from <i>L. paracasei</i> EPS DA-BACS had a ropy phenotype, which is known to have potential health benefits and is identified as loosely cell-bounded glucomannan-type EPS with a molecular size of 3.7 × 10<sup>6</sup> Da. EPS promoted the acid tolerance of <i>L. paracasei</i> EPS DA-BACS and provided cells with tolerance to gastrointestinal stress. The purified EPS showed growth inhibitory activity against <i>Clostridium difficile. L. paracasei</i> EPS DA-BACS cells completely inhibited the growth of <i>Bacillus subtilis</i>, <i>Pseudomonas aeruginosa</i>, and <i>Aspergillus brasiliensis</i>, as well as showed high growth inhibitory activity against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. Treatment of lipopolysaccharide-stimulated RAW 264.7 cells with heat-killed <i>L. paracasei</i> EPS DA-BACS cells led to a decrease in the production of nitric oxide, indicating the anti-inflammatory activity of <i>L. paracasei</i> EPS DA-BACS. Purified EPS promoted the growth of <i>Lactobacillus gasseri, Bifidobacterium bifidum, B. animalis</i>, and <i>B. faecale</i> which showed high prebiotic activity. <i>L. paracasei</i> EPS DA-BACS harbors no antibiotic resistance genes or virulence factors. Therefore, <i>L. paracasei</i> EPS DA-BACS exhibits anti-inflammatory and antimicrobial activities with high gut adhesion ability and gastrointestinal tolerance and can be used as a potential probiotic.https://www.mdpi.com/2076-2607/10/12/2431exopolysaccharide<i>Lacticaseibacillus paracasei</i>probioticslactic acid bacteriaanti-inflammatory activitygastrointestinal tolerance |
| spellingShingle | Min-Gyu Lee Huijin Joeng Jaein Shin Suin Kim Chaeeun Lee Youngbo Song Byung-Hoo Lee Hyoung-Geun Park Tae-Ho Lee Hai-Hua Jiang Young-Sun Han Bong-Gyeong Lee Ho-Jin Lee Min-Ju Park Yun-Ju Jun Young-Seo Park Potential Probiotic Properties of Exopolysaccharide-Producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide Microorganisms exopolysaccharide <i>Lacticaseibacillus paracasei</i> probiotics lactic acid bacteria anti-inflammatory activity gastrointestinal tolerance |
| title | Potential Probiotic Properties of Exopolysaccharide-Producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide |
| title_full | Potential Probiotic Properties of Exopolysaccharide-Producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide |
| title_fullStr | Potential Probiotic Properties of Exopolysaccharide-Producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide |
| title_full_unstemmed | Potential Probiotic Properties of Exopolysaccharide-Producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide |
| title_short | Potential Probiotic Properties of Exopolysaccharide-Producing <i>Lacticaseibacillus paracasei</i> EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide |
| title_sort | potential probiotic properties of exopolysaccharide producing i lacticaseibacillus paracasei i eps da bacs and prebiotic activity of its exopolysaccharide |
| topic | exopolysaccharide <i>Lacticaseibacillus paracasei</i> probiotics lactic acid bacteria anti-inflammatory activity gastrointestinal tolerance |
| url | https://www.mdpi.com/2076-2607/10/12/2431 |
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