A Comparative Study about the Neuroprotective Effects of DHA-Enriched Phosphatidylserine and EPA-Enriched Phosphatidylserine against Oxidative Damage in Primary Hippocampal Neurons
Nerve damage caused by accumulated oxidative stress is one of the characteristics and main mechanisms of Alzheimer’s disease (AD). Previous studies have shown that phosphatidylserine (PS) rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) plays a significant role in preventing and mi...
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MDPI AG
2023-07-01
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| author | Yi-Wen Wang Qian Li Xiao-Yue Li Ying-Cai Zhao Cheng-Cheng Wang Chang-Hu Xue Yu-Ming Wang Tian-Tian Zhang |
| author_facet | Yi-Wen Wang Qian Li Xiao-Yue Li Ying-Cai Zhao Cheng-Cheng Wang Chang-Hu Xue Yu-Ming Wang Tian-Tian Zhang |
| author_sort | Yi-Wen Wang |
| collection | DOAJ |
| description | Nerve damage caused by accumulated oxidative stress is one of the characteristics and main mechanisms of Alzheimer’s disease (AD). Previous studies have shown that phosphatidylserine (PS) rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) plays a significant role in preventing and mitigating the progression of AD. However, whether DHA-PS and EPA-PS can directly protect primary hippocampal neurons against oxidative damage has not been studied. Here, the neuroprotective functions of DHA-PS and EPA-PS against H<sub>2</sub>O<sub>2</sub>/t-BHP-induced oxidative damage and the possible mechanisms were evaluated in primary hippocampal neurons. It was found that DHA-PS and EPA-PS could significantly improve cell morphology and promote the restoration of neural network structure. Further studies showed that both of them significantly alleviated oxidative stress-mediated mitochondrial dysfunction. EPA-PS significantly inhibited the phosphorylation of ERK, thus playing an anti-apoptotic role, and EPA-PS significantly increased the protein expressions of p-TrkB and p-CREB, thus playing a neuroprotective role. In addition, EPA-PS, rather than DHA-PS could enhance synaptic plasticity by increasing the expression of SYN, and both could significantly reduce the expression levels of p-GSK3β and p-Tau. These results provide a scientific basis for the use of DHA/EPA-enriched phospholipids in the treatment of neurodegenerative diseases, and also provide a reference for the development of related functional foods. |
| first_indexed | 2024-03-11T00:52:34Z |
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| id | doaj.art-1f04ac689e6f47aebf6d11df6a79283b |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T00:52:34Z |
| publishDate | 2023-07-01 |
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| series | Marine Drugs |
| spelling | doaj.art-1f04ac689e6f47aebf6d11df6a79283b2023-11-18T20:14:26ZengMDPI AGMarine Drugs1660-33972023-07-0121741010.3390/md21070410A Comparative Study about the Neuroprotective Effects of DHA-Enriched Phosphatidylserine and EPA-Enriched Phosphatidylserine against Oxidative Damage in Primary Hippocampal NeuronsYi-Wen Wang0Qian Li1Xiao-Yue Li2Ying-Cai Zhao3Cheng-Cheng Wang4Chang-Hu Xue5Yu-Ming Wang6Tian-Tian Zhang7College of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266404, ChinaNerve damage caused by accumulated oxidative stress is one of the characteristics and main mechanisms of Alzheimer’s disease (AD). Previous studies have shown that phosphatidylserine (PS) rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) plays a significant role in preventing and mitigating the progression of AD. However, whether DHA-PS and EPA-PS can directly protect primary hippocampal neurons against oxidative damage has not been studied. Here, the neuroprotective functions of DHA-PS and EPA-PS against H<sub>2</sub>O<sub>2</sub>/t-BHP-induced oxidative damage and the possible mechanisms were evaluated in primary hippocampal neurons. It was found that DHA-PS and EPA-PS could significantly improve cell morphology and promote the restoration of neural network structure. Further studies showed that both of them significantly alleviated oxidative stress-mediated mitochondrial dysfunction. EPA-PS significantly inhibited the phosphorylation of ERK, thus playing an anti-apoptotic role, and EPA-PS significantly increased the protein expressions of p-TrkB and p-CREB, thus playing a neuroprotective role. In addition, EPA-PS, rather than DHA-PS could enhance synaptic plasticity by increasing the expression of SYN, and both could significantly reduce the expression levels of p-GSK3β and p-Tau. These results provide a scientific basis for the use of DHA/EPA-enriched phospholipids in the treatment of neurodegenerative diseases, and also provide a reference for the development of related functional foods.https://www.mdpi.com/1660-3397/21/7/410Alzheimer’s diseaseoxidative stressDHA/EPAphosphatidylserineprimary hippocampal neurons |
| spellingShingle | Yi-Wen Wang Qian Li Xiao-Yue Li Ying-Cai Zhao Cheng-Cheng Wang Chang-Hu Xue Yu-Ming Wang Tian-Tian Zhang A Comparative Study about the Neuroprotective Effects of DHA-Enriched Phosphatidylserine and EPA-Enriched Phosphatidylserine against Oxidative Damage in Primary Hippocampal Neurons Marine Drugs Alzheimer’s disease oxidative stress DHA/EPA phosphatidylserine primary hippocampal neurons |
| title | A Comparative Study about the Neuroprotective Effects of DHA-Enriched Phosphatidylserine and EPA-Enriched Phosphatidylserine against Oxidative Damage in Primary Hippocampal Neurons |
| title_full | A Comparative Study about the Neuroprotective Effects of DHA-Enriched Phosphatidylserine and EPA-Enriched Phosphatidylserine against Oxidative Damage in Primary Hippocampal Neurons |
| title_fullStr | A Comparative Study about the Neuroprotective Effects of DHA-Enriched Phosphatidylserine and EPA-Enriched Phosphatidylserine against Oxidative Damage in Primary Hippocampal Neurons |
| title_full_unstemmed | A Comparative Study about the Neuroprotective Effects of DHA-Enriched Phosphatidylserine and EPA-Enriched Phosphatidylserine against Oxidative Damage in Primary Hippocampal Neurons |
| title_short | A Comparative Study about the Neuroprotective Effects of DHA-Enriched Phosphatidylserine and EPA-Enriched Phosphatidylserine against Oxidative Damage in Primary Hippocampal Neurons |
| title_sort | comparative study about the neuroprotective effects of dha enriched phosphatidylserine and epa enriched phosphatidylserine against oxidative damage in primary hippocampal neurons |
| topic | Alzheimer’s disease oxidative stress DHA/EPA phosphatidylserine primary hippocampal neurons |
| url | https://www.mdpi.com/1660-3397/21/7/410 |
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