A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR
Fast and precise identification of microorganisms in the early diagnosis of sepsis is crucial for enhancing patient outcomes. Digital PCR (dPCR) is a highly sensitive approach for absolute quantification that can be utilized as a culture-independent molecular technique for diagnosing sepsis pathogen...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024035540 |
| _version_ | 1797224249292750848 |
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| author | Zhijun Zhao Yixuan Wang Yuting Kang Geng Wu Jing He Zhanying Wang Ju Yang Yaqi Wang Xiaojun Yang Wei Jia |
| author_facet | Zhijun Zhao Yixuan Wang Yuting Kang Geng Wu Jing He Zhanying Wang Ju Yang Yaqi Wang Xiaojun Yang Wei Jia |
| author_sort | Zhijun Zhao |
| collection | DOAJ |
| description | Fast and precise identification of microorganisms in the early diagnosis of sepsis is crucial for enhancing patient outcomes. Digital PCR (dPCR) is a highly sensitive approach for absolute quantification that can be utilized as a culture-independent molecular technique for diagnosing sepsis pathogens. We performed a retrospective investigation on 69 ICU patients suspected of sepsis. Our findings showed that a multiplex dPCR diagnostic kit outperformed blood culture in detecting the 15 most frequent bacteria that cause sepsis. Ninety-two bacterial strains were identified using dPCR at concentrations varying from 34 copies/mL to 105,800 copies/mL. The detection rate of dPCR was much greater than that of BC, with 27.53% (19/69) versus 73.91% (51/69). The sensitivity of dPCR was 63.2%. Our research indicated that dPCR outperforms blood culture in the early detection of sepsis-causing microorganisms. The diagnostic kit can detect a greater variety of pathogens with quantitative data, including polymicrobial infections, and has a quicker processing time. DPCR is a valuable technique that could aid in the proper management of sepsis. |
| first_indexed | 2024-04-24T13:50:07Z |
| format | Article |
| id | doaj.art-1f05b1dc8ec8445c910970fac45d9cd6 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:50:07Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-1f05b1dc8ec8445c910970fac45d9cd62024-04-04T05:05:02ZengElsevierHeliyon2405-84402024-03-01106e27523A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCRZhijun Zhao0Yixuan Wang1Yuting Kang2Geng Wu3Jing He4Zhanying Wang5Ju Yang6Yaqi Wang7Xiaojun Yang8Wei Jia9Medical Laboratory Center, General Hospital of Ningxia Medical University, Yinchuan, China; Ningxia Key Laboratory of Clinical Pathogenic Microorganisms, Yinchuan, ChinaSchool of Clinical Medicine, Ningxia Medical University, Yinchuan, China; Ningxia Key Laboratory of Clinical Pathogenic Microorganisms, Yinchuan, ChinaNingxia Key Laboratory of Clinical Pathogenic Microorganisms, Yinchuan, ChinaSchool of Clinical Medicine, Ningxia Medical University, Yinchuan, ChinaDepartment of Research and Development, Rainsure Scientific Co. Ltd., Suzhou, ChinaDepartment of Research and Development, Rainsure Scientific Co. Ltd., Suzhou, ChinaDepartment of Research and Development, Rainsure Scientific Co. Ltd., Suzhou, ChinaDepartment of Research and Development, Rainsure Scientific Co. Ltd., Suzhou, ChinaDepartment of Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchuan, ChinaMedical Laboratory Center, General Hospital of Ningxia Medical University, Yinchuan, China; Ningxia Key Laboratory of Clinical Pathogenic Microorganisms, Yinchuan, China; Corresponding author. Medical Laboratory Center, General Hospital of Ningxia Medical University, Yinchuan, China.Fast and precise identification of microorganisms in the early diagnosis of sepsis is crucial for enhancing patient outcomes. Digital PCR (dPCR) is a highly sensitive approach for absolute quantification that can be utilized as a culture-independent molecular technique for diagnosing sepsis pathogens. We performed a retrospective investigation on 69 ICU patients suspected of sepsis. Our findings showed that a multiplex dPCR diagnostic kit outperformed blood culture in detecting the 15 most frequent bacteria that cause sepsis. Ninety-two bacterial strains were identified using dPCR at concentrations varying from 34 copies/mL to 105,800 copies/mL. The detection rate of dPCR was much greater than that of BC, with 27.53% (19/69) versus 73.91% (51/69). The sensitivity of dPCR was 63.2%. Our research indicated that dPCR outperforms blood culture in the early detection of sepsis-causing microorganisms. The diagnostic kit can detect a greater variety of pathogens with quantitative data, including polymicrobial infections, and has a quicker processing time. DPCR is a valuable technique that could aid in the proper management of sepsis.http://www.sciencedirect.com/science/article/pii/S2405844024035540SepsisPathogensDPCRBlood cultureAntimicrobial therapy |
| spellingShingle | Zhijun Zhao Yixuan Wang Yuting Kang Geng Wu Jing He Zhanying Wang Ju Yang Yaqi Wang Xiaojun Yang Wei Jia A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR Heliyon Sepsis Pathogens DPCR Blood culture Antimicrobial therapy |
| title | A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR |
| title_full | A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR |
| title_fullStr | A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR |
| title_full_unstemmed | A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR |
| title_short | A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR |
| title_sort | retrospective study of the detection of sepsis pathogens comparing blood culture and culture independent digital pcr |
| topic | Sepsis Pathogens DPCR Blood culture Antimicrobial therapy |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024035540 |
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