Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana
Multidrug resistance, especially carbapenem resistance in Acinetobacter bacteria is a global healthcare concern. However, available data on the phenotypic and genotypic characteristics of Acinetobacter isolates from West Africa, including Ghana is scanty. Our aim was to investigate the antibiotic re...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.587398/full |
| _version_ | 1818245251315269632 |
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| author | Alafate Ayibieke Ayumi Kobayashi Masato Suzuki Wakana Sato Samiratu Mahazu Samiratu Mahazu Isaac Prah Isaac Prah Miyuki Mizoguchi Kyoji Moriya Takaya Hayashi Toshihiko Suzuki Shiroh Iwanaga Anthony Ablordey Ryoichi Saito |
| author_facet | Alafate Ayibieke Ayumi Kobayashi Masato Suzuki Wakana Sato Samiratu Mahazu Samiratu Mahazu Isaac Prah Isaac Prah Miyuki Mizoguchi Kyoji Moriya Takaya Hayashi Toshihiko Suzuki Shiroh Iwanaga Anthony Ablordey Ryoichi Saito |
| author_sort | Alafate Ayibieke |
| collection | DOAJ |
| description | Multidrug resistance, especially carbapenem resistance in Acinetobacter bacteria is a global healthcare concern. However, available data on the phenotypic and genotypic characteristics of Acinetobacter isolates from West Africa, including Ghana is scanty. Our aim was to investigate the antibiotic resistance profile and genotypic characteristics of Acinetobacter isolates from Ghana and to characterize carbapenemase producers using whole-genome sequencing (WGS). A total of 36 Acinetobacter isolates collected at three hospitals in Ghana between 2016 and 2017 were analyzed. MICs were determined by commercial antibiotic plates. Acinetobacter baumannii MLST was determined using the Pasteur scheme. WGS of OXA-carbapenemase producers was performed using short- and long-read sequencing strategies. The resistance rate was highest for trimethoprim/sulfamethoxazole (n = 22; 61%). Six (16.7%) and eight (22.2%) isolates were resistant to ceftazidime and colistin, respectively. Two (5.6%) isolates were resistant and one (2.8%) isolate had intermediate sensitivity to three carbapenems. Fifteen STs were identified in 24 A. baumannii isolates including six new STs (ST1467 ∼ ST1472). ST78 was the predominant (n = 6) followed by ST1469 (n = 3). Four carbapenemase-producing A. baumannii isolates also were identified. Isogenic ST103 isolates Ab-B004d-c and Ab-D10a-a harbored blaOXA–23 within Tn2007 on identical plasmids, pAb-B004d-c_3, and pAb-D10a-a_3. ST1472 isolate Ab-C102 and ST107 isolate Ab-C63 carried blaOXA–58 and blaOXA–420, a rare blaOXA–58 variant, respectively, within novel genetic contexts. Our results show that A. baumannii isolates of diverse and unique genotypes, including OXA-carbapenemase producers, are circulating in Ghana highlighting the need for a wider surveillance of antimicrobial resistance. |
| first_indexed | 2024-12-12T14:29:57Z |
| format | Article |
| id | doaj.art-1f08a331bfd24008a630377f3a483c41 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T14:29:57Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1f08a331bfd24008a630377f3a483c412022-12-22T00:21:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-11-011110.3389/fmicb.2020.587398587398Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From GhanaAlafate Ayibieke0Ayumi Kobayashi1Masato Suzuki2Wakana Sato3Samiratu Mahazu4Samiratu Mahazu5Isaac Prah6Isaac Prah7Miyuki Mizoguchi8Kyoji Moriya9Takaya Hayashi10Toshihiko Suzuki11Shiroh Iwanaga12Anthony Ablordey13Ryoichi Saito14Department of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanAntimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, JapanDepartment of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Environmental Parasitology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Molecular Virology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, JapanDepartment of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, JapanDepartment of Molecular Virology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Bacterial Pathogenesis, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Environmental Parasitology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaDepartment of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanMultidrug resistance, especially carbapenem resistance in Acinetobacter bacteria is a global healthcare concern. However, available data on the phenotypic and genotypic characteristics of Acinetobacter isolates from West Africa, including Ghana is scanty. Our aim was to investigate the antibiotic resistance profile and genotypic characteristics of Acinetobacter isolates from Ghana and to characterize carbapenemase producers using whole-genome sequencing (WGS). A total of 36 Acinetobacter isolates collected at three hospitals in Ghana between 2016 and 2017 were analyzed. MICs were determined by commercial antibiotic plates. Acinetobacter baumannii MLST was determined using the Pasteur scheme. WGS of OXA-carbapenemase producers was performed using short- and long-read sequencing strategies. The resistance rate was highest for trimethoprim/sulfamethoxazole (n = 22; 61%). Six (16.7%) and eight (22.2%) isolates were resistant to ceftazidime and colistin, respectively. Two (5.6%) isolates were resistant and one (2.8%) isolate had intermediate sensitivity to three carbapenems. Fifteen STs were identified in 24 A. baumannii isolates including six new STs (ST1467 ∼ ST1472). ST78 was the predominant (n = 6) followed by ST1469 (n = 3). Four carbapenemase-producing A. baumannii isolates also were identified. Isogenic ST103 isolates Ab-B004d-c and Ab-D10a-a harbored blaOXA–23 within Tn2007 on identical plasmids, pAb-B004d-c_3, and pAb-D10a-a_3. ST1472 isolate Ab-C102 and ST107 isolate Ab-C63 carried blaOXA–58 and blaOXA–420, a rare blaOXA–58 variant, respectively, within novel genetic contexts. Our results show that A. baumannii isolates of diverse and unique genotypes, including OXA-carbapenemase producers, are circulating in Ghana highlighting the need for a wider surveillance of antimicrobial resistance.https://www.frontiersin.org/articles/10.3389/fmicb.2020.587398/fullcarbapenem-hydrolyzing class D β-lactamasescarbapenem-resistant Acinetobactermultilocus sequence typingwhole-genome sequencingAcinetobacter baumanniiOXA-type beta-lactamase |
| spellingShingle | Alafate Ayibieke Ayumi Kobayashi Masato Suzuki Wakana Sato Samiratu Mahazu Samiratu Mahazu Isaac Prah Isaac Prah Miyuki Mizoguchi Kyoji Moriya Takaya Hayashi Toshihiko Suzuki Shiroh Iwanaga Anthony Ablordey Ryoichi Saito Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana Frontiers in Microbiology carbapenem-hydrolyzing class D β-lactamases carbapenem-resistant Acinetobacter multilocus sequence typing whole-genome sequencing Acinetobacter baumannii OXA-type beta-lactamase |
| title | Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana |
| title_full | Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana |
| title_fullStr | Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana |
| title_full_unstemmed | Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana |
| title_short | Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana |
| title_sort | prevalence and characterization of carbapenem hydrolyzing class d β lactamase producing acinetobacter isolates from ghana |
| topic | carbapenem-hydrolyzing class D β-lactamases carbapenem-resistant Acinetobacter multilocus sequence typing whole-genome sequencing Acinetobacter baumannii OXA-type beta-lactamase |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.587398/full |
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