Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules
The development of microfluidics-based systems in the recent years has provided a rapid and controlled method for the generation of monodisperse microencapsulates for multiple applications. Here, we explore the design, manufacture and characterization of a low-cost microsystem for the encapsulation...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/5/1158 |
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| author | Ana Lucia Campaña Diana Camila Sotelo Hector Alfonso Oliva Andres Aranguren Nancy Ornelas-Soto Juan C. Cruz Johann F. Osma |
| author_facet | Ana Lucia Campaña Diana Camila Sotelo Hector Alfonso Oliva Andres Aranguren Nancy Ornelas-Soto Juan C. Cruz Johann F. Osma |
| author_sort | Ana Lucia Campaña |
| collection | DOAJ |
| description | The development of microfluidics-based systems in the recent years has provided a rapid and controlled method for the generation of monodisperse microencapsulates for multiple applications. Here, we explore the design, manufacture and characterization of a low-cost microsystem for the encapsulation of the fungal laccase from <i>Pycnoporus sanguineus</i> CS43 in alginate microcapsules. Multiphysics simulations were used to overview the fluid behavior within the device and estimate the resulting capsule size. Polymethylmethacrylate (PMMA) sheets were used for final microsystem manufacture. Different flow rates of the continuous (Q<sub>c</sub>) and discrete (Q<sub>d</sub>) phases in the ranges of 83–293 mL/h and 1–5 mL/h, respectively, were evaluated for microcapsule fabrication. Universal Serial Bus (USB) microscope and image analysis was used to measure the final particle size. Laccase encapsulation was evaluated using spectrophotometry and with the aid of fluorescent dyes and confocal microscopy. Results showed microcapsule size was in the range of 203.13–716.00 μm and Q<sub>c</sub> was found as the dominant parameter to control capsule size. There was an effective enzyme encapsulation of 65.94% with respect to the initial laccase solution. |
| first_indexed | 2024-03-10T19:44:26Z |
| format | Article |
| id | doaj.art-1f0bc108005142d186cc84107d4d6a2c |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T19:44:26Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-1f0bc108005142d186cc84107d4d6a2c2023-11-20T00:54:18ZengMDPI AGPolymers2073-43602020-05-01125115810.3390/polym12051158Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse MicrocapsulesAna Lucia Campaña0Diana Camila Sotelo1Hector Alfonso Oliva2Andres Aranguren3Nancy Ornelas-Soto4Juan C. Cruz5Johann F. Osma6Department of Electrical and Electronic Engineering, Universidad de los Andes, Cra. 1E No. 19a-40, Bogotá D.C. 111711, ColombiaDepartment of Electrical and Electronic Engineering, Universidad de los Andes, Cra. 1E No. 19a-40, Bogotá D.C. 111711, ColombiaDepartment of Electrical and Electronic Engineering, Universidad de los Andes, Cra. 1E No. 19a-40, Bogotá D.C. 111711, ColombiaDepartment of Electrical and Electronic Engineering, Universidad de los Andes, Cra. 1E No. 19a-40, Bogotá D.C. 111711, ColombiaLaboratorio de Nanotecnología Ambiental, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Monterrey 64849, MexicoDepartment of Biomedical Engineering, Universidad de Los Andes, Cra. 1E No. 19a-40, Bogotá D.C. 111711, ColombiaDepartment of Electrical and Electronic Engineering, Universidad de los Andes, Cra. 1E No. 19a-40, Bogotá D.C. 111711, ColombiaThe development of microfluidics-based systems in the recent years has provided a rapid and controlled method for the generation of monodisperse microencapsulates for multiple applications. Here, we explore the design, manufacture and characterization of a low-cost microsystem for the encapsulation of the fungal laccase from <i>Pycnoporus sanguineus</i> CS43 in alginate microcapsules. Multiphysics simulations were used to overview the fluid behavior within the device and estimate the resulting capsule size. Polymethylmethacrylate (PMMA) sheets were used for final microsystem manufacture. Different flow rates of the continuous (Q<sub>c</sub>) and discrete (Q<sub>d</sub>) phases in the ranges of 83–293 mL/h and 1–5 mL/h, respectively, were evaluated for microcapsule fabrication. Universal Serial Bus (USB) microscope and image analysis was used to measure the final particle size. Laccase encapsulation was evaluated using spectrophotometry and with the aid of fluorescent dyes and confocal microscopy. Results showed microcapsule size was in the range of 203.13–716.00 μm and Q<sub>c</sub> was found as the dominant parameter to control capsule size. There was an effective enzyme encapsulation of 65.94% with respect to the initial laccase solution.https://www.mdpi.com/2073-4360/12/5/1158microfluidicsmicrocapsulesalginatelaccase |
| spellingShingle | Ana Lucia Campaña Diana Camila Sotelo Hector Alfonso Oliva Andres Aranguren Nancy Ornelas-Soto Juan C. Cruz Johann F. Osma Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules Polymers microfluidics microcapsules alginate laccase |
| title | Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules |
| title_full | Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules |
| title_fullStr | Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules |
| title_full_unstemmed | Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules |
| title_short | Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules |
| title_sort | fabrication and characterization of a low cost microfluidic system for the manufacture of alginate lacasse microcapsules |
| topic | microfluidics microcapsules alginate laccase |
| url | https://www.mdpi.com/2073-4360/12/5/1158 |
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