Distribution of Hepatitis B virus genotypes among healthy blood donors in eastern part of North India
<b>Aim:</b> We evaluated the distribution HBV genotypes among non-remunerated healthy blood donors in eastern North India. <b>Materials and Methods:</b> During screening of donated blood, 176 consecutive HBsAg positive, samples comprised the study. HBV-DNA was quantitative de...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2011-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | http://www.ajts.org/article.asp?issn=0973-6247;year=2011;volume=5;issue=2;spage=144;epage=149;aulast=Kumar |
| _version_ | 1811303972524261376 |
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| author | Kumar Kailash Kumar Manoj Rahaman Sk Singh T Patel Saurabh Nath Gopal |
| author_facet | Kumar Kailash Kumar Manoj Rahaman Sk Singh T Patel Saurabh Nath Gopal |
| author_sort | Kumar Kailash |
| collection | DOAJ |
| description | <b>Aim:</b> We evaluated the distribution HBV genotypes among non-remunerated healthy blood donors in eastern North India. <b>Materials and Methods:</b> During screening of donated blood, 176 consecutive HBsAg positive, samples comprised the study. HBV-DNA was quantitative detected in 150 samples by PCR. HBV genotype was determined by identifying genotype-specific DNA band using nested PCR. <b>Results:</b> Majorities were of age group 31-40 yrs (65.3%). Males (92.7%) outnumbered females (7.3%) and were HbeAg-negative HBsAg carriers. Over all, genotype-A was the most prevalent (54%) followed by D (21.3%). We did not find genotype-G and H. Districts under study, divided into four zones: Zone-I genotype-A was most common (62.3%) followed by D (18.8%); Zone-II genotype-C (41.2%) was more frequent followed by D (20.6% and A (17.7%). Zone-III in adjoining Bihar state close to Zone-I, A was more prevalent (81.8%) followed by B and C (9.1%). In Zone-IV adjoining Zone- II had genotype-A (100%) only. Genotype-D had more sporadic distribution. Genotype-E and F were prevalent in Zone I and II (3/150, 2%). <b>Conclusions:</b> Among blood donors HBV genotype-A followed by D was the most prevalent in eastern North India. Genotype-A had pattern of distribution signifying common focus, while D was more sporadic and C had single large pocket (Zone-II) probably common focus but restricting to particular area. Evidences are suggestive of association of HBV genotype in liver dysfunction. An effective treatment and preventive strategies based of genotypes will reduce the disease burden and increase the blood safety. |
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| format | Article |
| id | doaj.art-1f0c07853ba54862a94fec968c05e7eb |
| institution | Directory Open Access Journal |
| issn | 0973-6247 1998-3565 |
| language | English |
| last_indexed | 2024-04-13T07:57:42Z |
| publishDate | 2011-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj.art-1f0c07853ba54862a94fec968c05e7eb2022-12-22T02:55:21ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652011-01-0152144149Distribution of Hepatitis B virus genotypes among healthy blood donors in eastern part of North IndiaKumar KailashKumar ManojRahaman SkSingh TPatel SaurabhNath Gopal<b>Aim:</b> We evaluated the distribution HBV genotypes among non-remunerated healthy blood donors in eastern North India. <b>Materials and Methods:</b> During screening of donated blood, 176 consecutive HBsAg positive, samples comprised the study. HBV-DNA was quantitative detected in 150 samples by PCR. HBV genotype was determined by identifying genotype-specific DNA band using nested PCR. <b>Results:</b> Majorities were of age group 31-40 yrs (65.3%). Males (92.7%) outnumbered females (7.3%) and were HbeAg-negative HBsAg carriers. Over all, genotype-A was the most prevalent (54%) followed by D (21.3%). We did not find genotype-G and H. Districts under study, divided into four zones: Zone-I genotype-A was most common (62.3%) followed by D (18.8%); Zone-II genotype-C (41.2%) was more frequent followed by D (20.6% and A (17.7%). Zone-III in adjoining Bihar state close to Zone-I, A was more prevalent (81.8%) followed by B and C (9.1%). In Zone-IV adjoining Zone- II had genotype-A (100%) only. Genotype-D had more sporadic distribution. Genotype-E and F were prevalent in Zone I and II (3/150, 2%). <b>Conclusions:</b> Among blood donors HBV genotype-A followed by D was the most prevalent in eastern North India. Genotype-A had pattern of distribution signifying common focus, while D was more sporadic and C had single large pocket (Zone-II) probably common focus but restricting to particular area. Evidences are suggestive of association of HBV genotype in liver dysfunction. An effective treatment and preventive strategies based of genotypes will reduce the disease burden and increase the blood safety.http://www.ajts.org/article.asp?issn=0973-6247;year=2011;volume=5;issue=2;spage=144;epage=149;aulast=KumarHepatitis B virusHBV in blood donorHBV in IndiaHepatitis B virus genotypePCR genotyping |
| spellingShingle | Kumar Kailash Kumar Manoj Rahaman Sk Singh T Patel Saurabh Nath Gopal Distribution of Hepatitis B virus genotypes among healthy blood donors in eastern part of North India Asian Journal of Transfusion Science Hepatitis B virus HBV in blood donor HBV in India Hepatitis B virus genotype PCR genotyping |
| title | Distribution of Hepatitis B virus genotypes among healthy blood donors in eastern part of North India |
| title_full | Distribution of Hepatitis B virus genotypes among healthy blood donors in eastern part of North India |
| title_fullStr | Distribution of Hepatitis B virus genotypes among healthy blood donors in eastern part of North India |
| title_full_unstemmed | Distribution of Hepatitis B virus genotypes among healthy blood donors in eastern part of North India |
| title_short | Distribution of Hepatitis B virus genotypes among healthy blood donors in eastern part of North India |
| title_sort | distribution of hepatitis b virus genotypes among healthy blood donors in eastern part of north india |
| topic | Hepatitis B virus HBV in blood donor HBV in India Hepatitis B virus genotype PCR genotyping |
| url | http://www.ajts.org/article.asp?issn=0973-6247;year=2011;volume=5;issue=2;spage=144;epage=149;aulast=Kumar |
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