NusG is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with NusA
NusA and NusG are transcription factors that stimulate RNA polymerase pausing in Bacillus subtilis. While NusA was known to function as an intrinsic termination factor in B. subtilis, the role of NusG in this process was unknown. To examine the individual and combinatorial roles that NusA and NusG p...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-04-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/61880 |
| _version_ | 1828107640594497536 |
|---|---|
| author | Zachary F Mandell Reid T Oshiro Alexander V Yakhnin Rishi Vishwakarma Mikhail Kashlev Daniel B Kearns Paul Babitzke |
| author_facet | Zachary F Mandell Reid T Oshiro Alexander V Yakhnin Rishi Vishwakarma Mikhail Kashlev Daniel B Kearns Paul Babitzke |
| author_sort | Zachary F Mandell |
| collection | DOAJ |
| description | NusA and NusG are transcription factors that stimulate RNA polymerase pausing in Bacillus subtilis. While NusA was known to function as an intrinsic termination factor in B. subtilis, the role of NusG in this process was unknown. To examine the individual and combinatorial roles that NusA and NusG play in intrinsic termination, Term-seq was conducted in wild type, NusA depletion, ΔnusG, and NusA depletion ΔnusG strains. We determined that NusG functions as an intrinsic termination factor that works alone and cooperatively with NusA to facilitate termination at 88% of the 1400 identified intrinsic terminators. Our results indicate that NusG stimulates a sequence-specific pause that assists in the completion of suboptimal terminator hairpins with weak terminal A-U and G-U base pairs at the bottom of the stem. Loss of NusA and NusG leads to global misregulation of gene expression and loss of NusG results in flagella and swimming motility defects. |
| first_indexed | 2024-04-11T10:34:18Z |
| format | Article |
| id | doaj.art-1f0c0d49764849a08ca298f3f24f699d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:34:18Z |
| publishDate | 2021-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-1f0c0d49764849a08ca298f3f24f699d2022-12-22T04:29:20ZengeLife Sciences Publications LtdeLife2050-084X2021-04-011010.7554/eLife.61880NusG is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with NusAZachary F Mandell0Reid T Oshiro1Alexander V Yakhnin2https://orcid.org/0000-0002-7313-7054Rishi Vishwakarma3Mikhail Kashlev4Daniel B Kearns5Paul Babitzke6https://orcid.org/0000-0003-2481-1062Department of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, University Park, United StatesDepartment of Biology, Indiana University, Bloomington, United StatesNCI RNA Biology Laboratory, Center for Cancer Research, NCI, Frederick, United StatesDepartment of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, University Park, United StatesNCI RNA Biology Laboratory, Center for Cancer Research, NCI, Frederick, United StatesDepartment of Biology, Indiana University, Bloomington, United StatesDepartment of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, University Park, United StatesNusA and NusG are transcription factors that stimulate RNA polymerase pausing in Bacillus subtilis. While NusA was known to function as an intrinsic termination factor in B. subtilis, the role of NusG in this process was unknown. To examine the individual and combinatorial roles that NusA and NusG play in intrinsic termination, Term-seq was conducted in wild type, NusA depletion, ΔnusG, and NusA depletion ΔnusG strains. We determined that NusG functions as an intrinsic termination factor that works alone and cooperatively with NusA to facilitate termination at 88% of the 1400 identified intrinsic terminators. Our results indicate that NusG stimulates a sequence-specific pause that assists in the completion of suboptimal terminator hairpins with weak terminal A-U and G-U base pairs at the bottom of the stem. Loss of NusA and NusG leads to global misregulation of gene expression and loss of NusG results in flagella and swimming motility defects.https://elifesciences.org/articles/61880NusGNusAtranscription terminationmotilitygene regulationTerm-seq |
| spellingShingle | Zachary F Mandell Reid T Oshiro Alexander V Yakhnin Rishi Vishwakarma Mikhail Kashlev Daniel B Kearns Paul Babitzke NusG is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with NusA eLife NusG NusA transcription termination motility gene regulation Term-seq |
| title | NusG is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with NusA |
| title_full | NusG is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with NusA |
| title_fullStr | NusG is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with NusA |
| title_full_unstemmed | NusG is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with NusA |
| title_short | NusG is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with NusA |
| title_sort | nusg is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with nusa |
| topic | NusG NusA transcription termination motility gene regulation Term-seq |
| url | https://elifesciences.org/articles/61880 |
| work_keys_str_mv | AT zacharyfmandell nusgisanintrinsictranscriptionterminationfactorthatstimulatesmotilityandcoordinatesgeneexpressionwithnusa AT reidtoshiro nusgisanintrinsictranscriptionterminationfactorthatstimulatesmotilityandcoordinatesgeneexpressionwithnusa AT alexandervyakhnin nusgisanintrinsictranscriptionterminationfactorthatstimulatesmotilityandcoordinatesgeneexpressionwithnusa AT rishivishwakarma nusgisanintrinsictranscriptionterminationfactorthatstimulatesmotilityandcoordinatesgeneexpressionwithnusa AT mikhailkashlev nusgisanintrinsictranscriptionterminationfactorthatstimulatesmotilityandcoordinatesgeneexpressionwithnusa AT danielbkearns nusgisanintrinsictranscriptionterminationfactorthatstimulatesmotilityandcoordinatesgeneexpressionwithnusa AT paulbabitzke nusgisanintrinsictranscriptionterminationfactorthatstimulatesmotilityandcoordinatesgeneexpressionwithnusa |