Astaxanthin-loaded polylactic acid-glycolic acid nanoparticles ameliorate ulcerative colitis through antioxidant effects
IntroductionAstaxanthin (AST) is a type of carotenoid with strong antioxidant effects. However, the development and use of AST are limited by its water insolubility and low bioavailability. This study aims to investigate whether AST@PLGA can inhibit UC and reveal its possible mechanism.MethodsWe tes...
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1267274/full |
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| author | Chunmei Li Chunmei Li Yu Zhou Meng Yuan Yawen Yang Ruilong Song Ruilong Song Gang Xu Gang Chen |
| author_facet | Chunmei Li Chunmei Li Yu Zhou Meng Yuan Yawen Yang Ruilong Song Ruilong Song Gang Xu Gang Chen |
| author_sort | Chunmei Li |
| collection | DOAJ |
| description | IntroductionAstaxanthin (AST) is a type of carotenoid with strong antioxidant effects. However, the development and use of AST are limited by its water insolubility and low bioavailability. This study aims to investigate whether AST@PLGA can inhibit UC and reveal its possible mechanism.MethodsWe tested the particle size, polydispersity index, and zeta potential of AST@PLGA. Then, the in vitro release and antioxidant capacity of AST@PLGA were tested. Finally, the mouse model of colitis was established and SOD, MDA, TNF-α, IL-1β, IL-6 and P38 as well as ERK were detected from mice.ResultsParticle size, polydispersity index and zeta potential of AST @PLGA were 66.78 ± 0.64 nm, 0.247 and -9.8 ± 0.53 mV, respectively, and were stable within 14 days. Then, it was observed that the AST@PLGA nanoparticles not only maintained the effect of AST but also had a sustained release effect. Experiments in mice showed that AST@PLGA effectively reduced MDA, TNF-α, IL-1β and IL-6 levels and increased SOD levels. AST@PLGA also downregulated the protein expression of P38 and ERK. The results showed the positive protective effect of AST@PLGA in inhibiting acute colitis.DiscussionAST@PLGA nanoparticles have good stability and alleviating effect in colitis, which could be functional foods in the future. |
| first_indexed | 2024-03-11T11:16:12Z |
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| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-11T11:16:12Z |
| publishDate | 2023-11-01 |
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| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-1f0e33cbaf9844fb942c2320a28b90f32023-11-11T01:24:08ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-11-011010.3389/fnut.2023.12672741267274Astaxanthin-loaded polylactic acid-glycolic acid nanoparticles ameliorate ulcerative colitis through antioxidant effectsChunmei Li0Chunmei Li1Yu Zhou2Meng Yuan3Yawen Yang4Ruilong Song5Ruilong Song6Gang Xu7Gang Chen8College of Tourism and Culinary Science, Yangzhou University, Yangzhou, ChinaKey Laboratory of Chinese Cuisine Intangible Cultural Heritage Technology Inheritance, Ministry of Culture and Tourism, Yangzhou University, Yangzhou, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, ChinaCollege of Tourism and Culinary Science, Yangzhou University, Yangzhou, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, ChinaInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, ChinaDepartment of Burn and Plastic Surgery, Northern Jiangsu People’s Hospital/Clinical Medical College, Yangzhou University, Yangzhou, ChinaSchool of Rehabilitation Science and Engineering, Qingdao Hospital (Qingdao Municipal Hospital), University of Health and Rehabilitation Sciences, Qingdao, ChinaIntroductionAstaxanthin (AST) is a type of carotenoid with strong antioxidant effects. However, the development and use of AST are limited by its water insolubility and low bioavailability. This study aims to investigate whether AST@PLGA can inhibit UC and reveal its possible mechanism.MethodsWe tested the particle size, polydispersity index, and zeta potential of AST@PLGA. Then, the in vitro release and antioxidant capacity of AST@PLGA were tested. Finally, the mouse model of colitis was established and SOD, MDA, TNF-α, IL-1β, IL-6 and P38 as well as ERK were detected from mice.ResultsParticle size, polydispersity index and zeta potential of AST @PLGA were 66.78 ± 0.64 nm, 0.247 and -9.8 ± 0.53 mV, respectively, and were stable within 14 days. Then, it was observed that the AST@PLGA nanoparticles not only maintained the effect of AST but also had a sustained release effect. Experiments in mice showed that AST@PLGA effectively reduced MDA, TNF-α, IL-1β and IL-6 levels and increased SOD levels. AST@PLGA also downregulated the protein expression of P38 and ERK. The results showed the positive protective effect of AST@PLGA in inhibiting acute colitis.DiscussionAST@PLGA nanoparticles have good stability and alleviating effect in colitis, which could be functional foods in the future.https://www.frontiersin.org/articles/10.3389/fnut.2023.1267274/fullastaxanthinpolylactic acid-glycolic acidantioxidationcolitisMAPK |
| spellingShingle | Chunmei Li Chunmei Li Yu Zhou Meng Yuan Yawen Yang Ruilong Song Ruilong Song Gang Xu Gang Chen Astaxanthin-loaded polylactic acid-glycolic acid nanoparticles ameliorate ulcerative colitis through antioxidant effects Frontiers in Nutrition astaxanthin polylactic acid-glycolic acid antioxidation colitis MAPK |
| title | Astaxanthin-loaded polylactic acid-glycolic acid nanoparticles ameliorate ulcerative colitis through antioxidant effects |
| title_full | Astaxanthin-loaded polylactic acid-glycolic acid nanoparticles ameliorate ulcerative colitis through antioxidant effects |
| title_fullStr | Astaxanthin-loaded polylactic acid-glycolic acid nanoparticles ameliorate ulcerative colitis through antioxidant effects |
| title_full_unstemmed | Astaxanthin-loaded polylactic acid-glycolic acid nanoparticles ameliorate ulcerative colitis through antioxidant effects |
| title_short | Astaxanthin-loaded polylactic acid-glycolic acid nanoparticles ameliorate ulcerative colitis through antioxidant effects |
| title_sort | astaxanthin loaded polylactic acid glycolic acid nanoparticles ameliorate ulcerative colitis through antioxidant effects |
| topic | astaxanthin polylactic acid-glycolic acid antioxidation colitis MAPK |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1267274/full |
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