Modulation of <i>Streptococcus mutans</i> Adherence to Hydroxyapatite by Engineered Salivary Peptides
Since the modification of the proteinaceous components of the Acquired Enamel Pellicle (AEP) could influence the adhesion of <i>Streptococcus mutans,</i> the most cariogenic bacteria, to dental surfaces, we assessed if engineered salivary peptides would affect the adherence and modulate...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/223 |
| _version_ | 1827653702222086144 |
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| author | Lina Maria Marin Yizhi Xiao Jaime Aparecido Cury Walter Luiz Siqueira |
| author_facet | Lina Maria Marin Yizhi Xiao Jaime Aparecido Cury Walter Luiz Siqueira |
| author_sort | Lina Maria Marin |
| collection | DOAJ |
| description | Since the modification of the proteinaceous components of the Acquired Enamel Pellicle (AEP) could influence the adhesion of <i>Streptococcus mutans,</i> the most cariogenic bacteria, to dental surfaces, we assessed if engineered salivary peptides would affect the adherence and modulate the bacterial proteome upon adherence. Single-component AEPs were formed onto hydroxyapatite (HAp) discs by incubating them with statherin, histatin-3, DR9, DR9-DR9, DR9-RR14, RR14, and parotid saliva. Then, the discs were inoculated with <i>S. mutans</i> UA159 and the bacteria were allowed to adhere for 2 h, 4 h, and 8 h (<i>n</i> = 12/treatment/time point). The number of bacteria adhered to the HAp discs was determined at each time point and analyzed by two-way ANOVA and Bonferroni tests. Cell-wall proteins were extracted from adhered, planktonic, and inoculum (baseline) bacteria and proteome profiles were obtained after a bottom-up proteomics approach. The number of adhered bacteria significantly increased over time, being the mean values obtained at 8 h, from highest to lowest, as follows: DR9-RR14 > statherin > RR14 = DR9-DR9 > DR9 = histatin3 > saliva (<i>p</i> < 0.05). Treatments modulated the bacterial proteome upon adherence. The findings suggested a potential use of our engineered peptide DR9-DR9 to control <i>S. mutans</i> biofilm development by reducing bacterial colonization. |
| first_indexed | 2024-03-09T21:24:22Z |
| format | Article |
| id | doaj.art-1f10eb7020984390838fc8995afdf3b2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:24:22Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1f10eb7020984390838fc8995afdf3b22023-11-23T21:12:46ZengMDPI AGMicroorganisms2076-26072022-01-0110222310.3390/microorganisms10020223Modulation of <i>Streptococcus mutans</i> Adherence to Hydroxyapatite by Engineered Salivary PeptidesLina Maria Marin0Yizhi Xiao1Jaime Aparecido Cury2Walter Luiz Siqueira3College of Dentistry, University of Saskatchewan, Saskatoon, SK S7N 5E4, CanadaSchulich School of Dentistry, The University of Western Ontario, London, ON N6A 5C1, CanadaPiracicaba Dental School, University of Campinas, Piracicaba CEP 13414-903, BrazilCollege of Dentistry, University of Saskatchewan, Saskatoon, SK S7N 5E4, CanadaSince the modification of the proteinaceous components of the Acquired Enamel Pellicle (AEP) could influence the adhesion of <i>Streptococcus mutans,</i> the most cariogenic bacteria, to dental surfaces, we assessed if engineered salivary peptides would affect the adherence and modulate the bacterial proteome upon adherence. Single-component AEPs were formed onto hydroxyapatite (HAp) discs by incubating them with statherin, histatin-3, DR9, DR9-DR9, DR9-RR14, RR14, and parotid saliva. Then, the discs were inoculated with <i>S. mutans</i> UA159 and the bacteria were allowed to adhere for 2 h, 4 h, and 8 h (<i>n</i> = 12/treatment/time point). The number of bacteria adhered to the HAp discs was determined at each time point and analyzed by two-way ANOVA and Bonferroni tests. Cell-wall proteins were extracted from adhered, planktonic, and inoculum (baseline) bacteria and proteome profiles were obtained after a bottom-up proteomics approach. The number of adhered bacteria significantly increased over time, being the mean values obtained at 8 h, from highest to lowest, as follows: DR9-RR14 > statherin > RR14 = DR9-DR9 > DR9 = histatin3 > saliva (<i>p</i> < 0.05). Treatments modulated the bacterial proteome upon adherence. The findings suggested a potential use of our engineered peptide DR9-DR9 to control <i>S. mutans</i> biofilm development by reducing bacterial colonization.https://www.mdpi.com/2076-2607/10/2/223salivary peptidesacquired enamel pellicleadherencestatherinhistatinproteomics |
| spellingShingle | Lina Maria Marin Yizhi Xiao Jaime Aparecido Cury Walter Luiz Siqueira Modulation of <i>Streptococcus mutans</i> Adherence to Hydroxyapatite by Engineered Salivary Peptides Microorganisms salivary peptides acquired enamel pellicle adherence statherin histatin proteomics |
| title | Modulation of <i>Streptococcus mutans</i> Adherence to Hydroxyapatite by Engineered Salivary Peptides |
| title_full | Modulation of <i>Streptococcus mutans</i> Adherence to Hydroxyapatite by Engineered Salivary Peptides |
| title_fullStr | Modulation of <i>Streptococcus mutans</i> Adherence to Hydroxyapatite by Engineered Salivary Peptides |
| title_full_unstemmed | Modulation of <i>Streptococcus mutans</i> Adherence to Hydroxyapatite by Engineered Salivary Peptides |
| title_short | Modulation of <i>Streptococcus mutans</i> Adherence to Hydroxyapatite by Engineered Salivary Peptides |
| title_sort | modulation of i streptococcus mutans i adherence to hydroxyapatite by engineered salivary peptides |
| topic | salivary peptides acquired enamel pellicle adherence statherin histatin proteomics |
| url | https://www.mdpi.com/2076-2607/10/2/223 |
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