Topical Administration of a Nanoformulation of Chitosan-Hyaluronic Acid-Epoetin Beta in a Rat Model of Glaucoma
The present work investigates the effects of chitosan-hyaluronic acid-epoetin beta (CS/HA-EPOβ) nanoparticles after topical ocular administration in a rat glaucoma model. Wistar Hannover rats (<i>n</i> = 24) were submitted to a complete ophthalmological examination and electroretinograph...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/1424-8247/16/2/164 |
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| author | Beatriz Silva Lídia M. Gonçalves Berta São Braz Esmeralda Delgado |
| author_facet | Beatriz Silva Lídia M. Gonçalves Berta São Braz Esmeralda Delgado |
| author_sort | Beatriz Silva |
| collection | DOAJ |
| description | The present work investigates the effects of chitosan-hyaluronic acid-epoetin beta (CS/HA-EPOβ) nanoparticles after topical ocular administration in a rat glaucoma model. Wistar Hannover rats (<i>n</i> = 24) were submitted to a complete ophthalmological examination and electroretinography, followed by glaucoma induction in their right eye on day 1 of the study. Treatment group (T) received CS/HA-EPOβ nanocarriers (<i>n</i> = 12), while the control group (C) received only empty ones. Electroretinography was repeated on day 3 (<i>n</i> = 24) and before euthanasia on day 7 (<i>n</i> = 8), 14 (<i>n</i> = 8), and 21 (<i>n</i> = 8), followed by bilateral enucleation and histological assessment. The animals showed good tolerance to the nanoformulation. Maximum IOP values on the right eye occurred shortly after glaucoma induction (T = 62.6 ± 8.3 mmHg; C = 63.6 ± 7.9 mmHg). Animals from the treated group presented a tendency for faster recovery of retinal electrical activity (<i>p</i> > 0.05). EPOβ was detected on the retina of all treated eyes using immunofluorescence. Control animals presented with thinner retinas compared to the treated ones (<i>p</i> < 0.05). Therefore, topical ocular administration of CS/HA-EPOβ nanoparticles enabled EPOβ delivery to the retina of glaucomatous rats and promoted an earlier retinal recovery, confirming EPOβ’s neuroprotective effects. The encouraging results of this preclinical study pave the way for new strategies for topical ocular administration of neuroprotective compounds. |
| first_indexed | 2024-03-11T08:17:37Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T08:17:37Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-1f19d5ac502349b7b26e71274b08f0cd2023-11-16T22:35:54ZengMDPI AGPharmaceuticals1424-82472023-01-0116216410.3390/ph16020164Topical Administration of a Nanoformulation of Chitosan-Hyaluronic Acid-Epoetin Beta in a Rat Model of GlaucomaBeatriz Silva0Lídia M. Gonçalves1Berta São Braz2Esmeralda Delgado3CIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Universidade de Lisboa Avenida da Universidade Técnica, 1300-477 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Universidade de Lisboa Avenida da Universidade Técnica, 1300-477 Lisbon, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Universidade de Lisboa Avenida da Universidade Técnica, 1300-477 Lisbon, PortugalThe present work investigates the effects of chitosan-hyaluronic acid-epoetin beta (CS/HA-EPOβ) nanoparticles after topical ocular administration in a rat glaucoma model. Wistar Hannover rats (<i>n</i> = 24) were submitted to a complete ophthalmological examination and electroretinography, followed by glaucoma induction in their right eye on day 1 of the study. Treatment group (T) received CS/HA-EPOβ nanocarriers (<i>n</i> = 12), while the control group (C) received only empty ones. Electroretinography was repeated on day 3 (<i>n</i> = 24) and before euthanasia on day 7 (<i>n</i> = 8), 14 (<i>n</i> = 8), and 21 (<i>n</i> = 8), followed by bilateral enucleation and histological assessment. The animals showed good tolerance to the nanoformulation. Maximum IOP values on the right eye occurred shortly after glaucoma induction (T = 62.6 ± 8.3 mmHg; C = 63.6 ± 7.9 mmHg). Animals from the treated group presented a tendency for faster recovery of retinal electrical activity (<i>p</i> > 0.05). EPOβ was detected on the retina of all treated eyes using immunofluorescence. Control animals presented with thinner retinas compared to the treated ones (<i>p</i> < 0.05). Therefore, topical ocular administration of CS/HA-EPOβ nanoparticles enabled EPOβ delivery to the retina of glaucomatous rats and promoted an earlier retinal recovery, confirming EPOβ’s neuroprotective effects. The encouraging results of this preclinical study pave the way for new strategies for topical ocular administration of neuroprotective compounds.https://www.mdpi.com/1424-8247/16/2/164glaucomaneuroprotectionnanoparticlesepoetin betachitosanhyaluronic acid |
| spellingShingle | Beatriz Silva Lídia M. Gonçalves Berta São Braz Esmeralda Delgado Topical Administration of a Nanoformulation of Chitosan-Hyaluronic Acid-Epoetin Beta in a Rat Model of Glaucoma Pharmaceuticals glaucoma neuroprotection nanoparticles epoetin beta chitosan hyaluronic acid |
| title | Topical Administration of a Nanoformulation of Chitosan-Hyaluronic Acid-Epoetin Beta in a Rat Model of Glaucoma |
| title_full | Topical Administration of a Nanoformulation of Chitosan-Hyaluronic Acid-Epoetin Beta in a Rat Model of Glaucoma |
| title_fullStr | Topical Administration of a Nanoformulation of Chitosan-Hyaluronic Acid-Epoetin Beta in a Rat Model of Glaucoma |
| title_full_unstemmed | Topical Administration of a Nanoformulation of Chitosan-Hyaluronic Acid-Epoetin Beta in a Rat Model of Glaucoma |
| title_short | Topical Administration of a Nanoformulation of Chitosan-Hyaluronic Acid-Epoetin Beta in a Rat Model of Glaucoma |
| title_sort | topical administration of a nanoformulation of chitosan hyaluronic acid epoetin beta in a rat model of glaucoma |
| topic | glaucoma neuroprotection nanoparticles epoetin beta chitosan hyaluronic acid |
| url | https://www.mdpi.com/1424-8247/16/2/164 |
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