The seed biochemical signature as a potent marker for water logging tolerance in maize
Plant breeders are in the need for convenient, reproducible, reliable and rapid screening methods to be used as a proxy for water logging tolerance for a large number of genotypes. Addressing this need, the present study was carried in order to identify potential biochemical markers in the seeds of...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-04-01
|
| Series: | Plant Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X22000306 |
| _version_ | 1819061965341851648 |
|---|---|
| author | Vishal Chugh Narinder Kaur Anil Kumar Gupta Ashutosh Rai |
| author_facet | Vishal Chugh Narinder Kaur Anil Kumar Gupta Ashutosh Rai |
| author_sort | Vishal Chugh |
| collection | DOAJ |
| description | Plant breeders are in the need for convenient, reproducible, reliable and rapid screening methods to be used as a proxy for water logging tolerance for a large number of genotypes. Addressing this need, the present study was carried in order to identify potential biochemical markers in the seeds of 10 contrasting maize (Zea mays L.) genotypes differing in their tolerance against water logging. Results clearly indicate that tolerant genotypes inherently exhibit better antioxidant system as evident by higher activities of catalase and glutathione reductase than the susceptible genotypes hence minimizes resulting oxidative stress. Significantly higher activities of anaerobic metabolism enzymes viz. alcohol dehydrogenase, aldehyde dehydrogenase and pyruvate decarboxylase in tolerant maize genotypes signifies efficient system for the recycling of NADH for the continuation of glycolysis and simultaneous detoxification of acetaldehyde thus provides prolonged survival of tolerant plants under water logging. Higher Sucrose synthase (SuSy) activity in tolerant genotypes over the susceptible genotypes indicates uninterrupted supply of reducing sugars for glycolytic activity. The content of total and reducing sugars content was found 27% higher in tolerant genotypes, which is in agreement with the SuSy activity. Soluble sugars analysis through reverse phase HPLC revealed higher relative concentration of fructose and glucose in tolerant maize genotypes than susceptible genotypes. Tolerant genotypes also exhibited significantly lower phytic acid and higher proline content. Hence the observed biochemical diversity could be useful for screening and development of water logging stress-tolerant genotypes of maize. |
| first_indexed | 2024-12-21T14:51:16Z |
| format | Article |
| id | doaj.art-1f1e9b3c92e445f5a87c5788784df339 |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-12-21T14:51:16Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-1f1e9b3c92e445f5a87c5788784df3392022-12-21T18:59:53ZengElsevierPlant Stress2667-064X2022-04-014100085The seed biochemical signature as a potent marker for water logging tolerance in maizeVishal Chugh0Narinder Kaur1Anil Kumar Gupta2Ashutosh Rai3Department of Biochemistry, Punjab Agricultural University, Ludhiana, India; Department of Biochemistry, College of Horticulture, Banda University of Agriculture and Technology, Banda, U.P., IndiaDepartment of Biochemistry, Punjab Agricultural University, Ludhiana, IndiaDepartment of Biochemistry, Punjab Agricultural University, Ludhiana, IndiaDepartment of Biochemistry, College of Horticulture, Banda University of Agriculture and Technology, Banda, U.P., India; Corresponding author.Plant breeders are in the need for convenient, reproducible, reliable and rapid screening methods to be used as a proxy for water logging tolerance for a large number of genotypes. Addressing this need, the present study was carried in order to identify potential biochemical markers in the seeds of 10 contrasting maize (Zea mays L.) genotypes differing in their tolerance against water logging. Results clearly indicate that tolerant genotypes inherently exhibit better antioxidant system as evident by higher activities of catalase and glutathione reductase than the susceptible genotypes hence minimizes resulting oxidative stress. Significantly higher activities of anaerobic metabolism enzymes viz. alcohol dehydrogenase, aldehyde dehydrogenase and pyruvate decarboxylase in tolerant maize genotypes signifies efficient system for the recycling of NADH for the continuation of glycolysis and simultaneous detoxification of acetaldehyde thus provides prolonged survival of tolerant plants under water logging. Higher Sucrose synthase (SuSy) activity in tolerant genotypes over the susceptible genotypes indicates uninterrupted supply of reducing sugars for glycolytic activity. The content of total and reducing sugars content was found 27% higher in tolerant genotypes, which is in agreement with the SuSy activity. Soluble sugars analysis through reverse phase HPLC revealed higher relative concentration of fructose and glucose in tolerant maize genotypes than susceptible genotypes. Tolerant genotypes also exhibited significantly lower phytic acid and higher proline content. Hence the observed biochemical diversity could be useful for screening and development of water logging stress-tolerant genotypes of maize.http://www.sciencedirect.com/science/article/pii/S2667064X22000306MaizeWater-loggingAntioxidantAnaerobic metabolismAlcohol dehydrogenaseSucrose synthase |
| spellingShingle | Vishal Chugh Narinder Kaur Anil Kumar Gupta Ashutosh Rai The seed biochemical signature as a potent marker for water logging tolerance in maize Plant Stress Maize Water-logging Antioxidant Anaerobic metabolism Alcohol dehydrogenase Sucrose synthase |
| title | The seed biochemical signature as a potent marker for water logging tolerance in maize |
| title_full | The seed biochemical signature as a potent marker for water logging tolerance in maize |
| title_fullStr | The seed biochemical signature as a potent marker for water logging tolerance in maize |
| title_full_unstemmed | The seed biochemical signature as a potent marker for water logging tolerance in maize |
| title_short | The seed biochemical signature as a potent marker for water logging tolerance in maize |
| title_sort | seed biochemical signature as a potent marker for water logging tolerance in maize |
| topic | Maize Water-logging Antioxidant Anaerobic metabolism Alcohol dehydrogenase Sucrose synthase |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X22000306 |
| work_keys_str_mv | AT vishalchugh theseedbiochemicalsignatureasapotentmarkerforwaterloggingtoleranceinmaize AT narinderkaur theseedbiochemicalsignatureasapotentmarkerforwaterloggingtoleranceinmaize AT anilkumargupta theseedbiochemicalsignatureasapotentmarkerforwaterloggingtoleranceinmaize AT ashutoshrai theseedbiochemicalsignatureasapotentmarkerforwaterloggingtoleranceinmaize AT vishalchugh seedbiochemicalsignatureasapotentmarkerforwaterloggingtoleranceinmaize AT narinderkaur seedbiochemicalsignatureasapotentmarkerforwaterloggingtoleranceinmaize AT anilkumargupta seedbiochemicalsignatureasapotentmarkerforwaterloggingtoleranceinmaize AT ashutoshrai seedbiochemicalsignatureasapotentmarkerforwaterloggingtoleranceinmaize |