A Novel Strategy for Screening Active Components in Cistanche tubulosa Based on Spectrum-Effect Relationship Analysis and Network Pharmacology
Cistanche tubulosa (Schenk) R. Wight is a valuable herbal medicine in China. The study aimed to explore the potential mechanisms of C. tubulosa on antioxidant activity using spectrum-effect relationship and network pharmacology and the possibilities of utilizing herbal dregs. In this work, different...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2023-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/9030015 |
| _version_ | 1811165381583175680 |
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| author | Xiao-Tong Liu Dong-Mei Sun Wen-Xin Yu Wei-Xiong Lin Liao-Yuan Liu Yu Zeng |
| author_facet | Xiao-Tong Liu Dong-Mei Sun Wen-Xin Yu Wei-Xiong Lin Liao-Yuan Liu Yu Zeng |
| author_sort | Xiao-Tong Liu |
| collection | DOAJ |
| description | Cistanche tubulosa (Schenk) R. Wight is a valuable herbal medicine in China. The study aimed to explore the potential mechanisms of C. tubulosa on antioxidant activity using spectrum-effect relationship and network pharmacology and the possibilities of utilizing herbal dregs. In this work, different extracts of C. tubulosa, including herbal materials, water extracts, and herbal residues, were evaluated using high-performance liquid chromatography (HPLC) technology. In addition, the antioxidant activities were estimated in vitro, including 2, 2-diphenyl-1-picrylhydrazyl; superoxide anion; and hydroxyl radical scavenging assays. The spectrum-effect relationships between the HPLC fingerprints and the biological capabilities were analyzed via partial least squares regression, bivariate correlation analysis, and redundancy analysis. Furthermore, network pharmacology was used to predict potential mechanisms of C. tubulosa in the treatment of antioxidant-related diseases. According to the results, eleven common peaks were shared by different extracts. Geniposidic acid, echinacoside, verbascoside, tubuloside A, and isoacteoside were quantified and compared among different forms of C. tubulosa. The spectrum-effect relationship study indicated that peak A6 might be the most decisive component among the three forms. Based on network pharmacology, there were 159 target genes shared by active components and antioxidant-related diseases. Targets related to antioxidant activity and relevant pathways were discussed. Our results provide a theoretical basis for recycling the herbal residues and the potential mechanisms of C. tubulosa in the treatment of antioxidant-related diseases. |
| first_indexed | 2024-04-10T15:37:25Z |
| format | Article |
| id | doaj.art-1f20fa1b88ff45df871a9044ed7b46ad |
| institution | Directory Open Access Journal |
| issn | 2090-8873 |
| language | English |
| last_indexed | 2024-04-10T15:37:25Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj.art-1f20fa1b88ff45df871a9044ed7b46ad2023-02-13T01:09:06ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88732023-01-01202310.1155/2023/9030015A Novel Strategy for Screening Active Components in Cistanche tubulosa Based on Spectrum-Effect Relationship Analysis and Network PharmacologyXiao-Tong Liu0Dong-Mei Sun1Wen-Xin Yu2Wei-Xiong Lin3Liao-Yuan Liu4Yu Zeng5School of Chinese Materia MedicaGuangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula GranuleSchool of Chinese Materia MedicaGuangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula GranuleGuangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula GranuleSchool of Chinese Materia MedicaCistanche tubulosa (Schenk) R. Wight is a valuable herbal medicine in China. The study aimed to explore the potential mechanisms of C. tubulosa on antioxidant activity using spectrum-effect relationship and network pharmacology and the possibilities of utilizing herbal dregs. In this work, different extracts of C. tubulosa, including herbal materials, water extracts, and herbal residues, were evaluated using high-performance liquid chromatography (HPLC) technology. In addition, the antioxidant activities were estimated in vitro, including 2, 2-diphenyl-1-picrylhydrazyl; superoxide anion; and hydroxyl radical scavenging assays. The spectrum-effect relationships between the HPLC fingerprints and the biological capabilities were analyzed via partial least squares regression, bivariate correlation analysis, and redundancy analysis. Furthermore, network pharmacology was used to predict potential mechanisms of C. tubulosa in the treatment of antioxidant-related diseases. According to the results, eleven common peaks were shared by different extracts. Geniposidic acid, echinacoside, verbascoside, tubuloside A, and isoacteoside were quantified and compared among different forms of C. tubulosa. The spectrum-effect relationship study indicated that peak A6 might be the most decisive component among the three forms. Based on network pharmacology, there were 159 target genes shared by active components and antioxidant-related diseases. Targets related to antioxidant activity and relevant pathways were discussed. Our results provide a theoretical basis for recycling the herbal residues and the potential mechanisms of C. tubulosa in the treatment of antioxidant-related diseases.http://dx.doi.org/10.1155/2023/9030015 |
| spellingShingle | Xiao-Tong Liu Dong-Mei Sun Wen-Xin Yu Wei-Xiong Lin Liao-Yuan Liu Yu Zeng A Novel Strategy for Screening Active Components in Cistanche tubulosa Based on Spectrum-Effect Relationship Analysis and Network Pharmacology Journal of Analytical Methods in Chemistry |
| title | A Novel Strategy for Screening Active Components in Cistanche tubulosa Based on Spectrum-Effect Relationship Analysis and Network Pharmacology |
| title_full | A Novel Strategy for Screening Active Components in Cistanche tubulosa Based on Spectrum-Effect Relationship Analysis and Network Pharmacology |
| title_fullStr | A Novel Strategy for Screening Active Components in Cistanche tubulosa Based on Spectrum-Effect Relationship Analysis and Network Pharmacology |
| title_full_unstemmed | A Novel Strategy for Screening Active Components in Cistanche tubulosa Based on Spectrum-Effect Relationship Analysis and Network Pharmacology |
| title_short | A Novel Strategy for Screening Active Components in Cistanche tubulosa Based on Spectrum-Effect Relationship Analysis and Network Pharmacology |
| title_sort | novel strategy for screening active components in cistanche tubulosa based on spectrum effect relationship analysis and network pharmacology |
| url | http://dx.doi.org/10.1155/2023/9030015 |
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