Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats
Adult-onset hypothyroidism induces a variety of impairments on hippocampus- dependent neurocognitive functioningin which many synaptic proteins in hippocampus neurons are involved. Here, we observed the effect of adult-onset hypothyroidism on the expression of syntaxin-1 and munc-18 in the dorsal hi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2012-05-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/1916 |
| _version_ | 1818514706117165056 |
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| author | Y. Zhu D. Ning F. Wang C. Liu Y. Xu X. Jia D. Zhu |
| author_facet | Y. Zhu D. Ning F. Wang C. Liu Y. Xu X. Jia D. Zhu |
| author_sort | Y. Zhu |
| collection | DOAJ |
| description | Adult-onset hypothyroidism induces a variety of impairments on hippocampus- dependent neurocognitive functioningin which many synaptic proteins in hippocampus neurons are involved. Here, we observed the effect of adult-onset hypothyroidism on the expression of syntaxin-1 and munc-18 in the dorsal hippocampus and whether the altered proteins could be restored by levothyroxine (T4) treatment. All rats were separated into 4 groups randomly: hypothyroid group, 5μg T4/100 g body weight (BW) treated group, 20 μg T4/100g BW treated group and control group. The radioimmunoassay kits were applied to assay the levels of serum T3 and T4, and the levels of syntaxin-1 and munc-18 in hippocampus were assessed by immunohistochemistry and Western blot. Both analysis corroborated that syntaxin-1 in the hypothyroid group was significantly higher. Munc-18 was lower in four layers of CA3 and dentate gyrus by immunohistochemistry. After two weeks of treatment with 5 μg T4/100g BW for hypothyroidism, syntaxin-1 levels were completely restored, whereas the recovery of munc-18 only located in two of the four impaired layers. Twenty μg T4/100g BW treatment normalized munc-18 levels. These data suggested that adult-onset hypothyroidism induced increment of syntaxin-1 and decrement of munc-18 in the dorsal hippocampus, which could be restored by T4 treatment. Larger dosage of T4 caused more effective restorations. |
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| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-11T00:19:26Z |
| publishDate | 2012-05-01 |
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| series | European Journal of Histochemistry |
| spelling | doaj.art-1f232f1e2ba8497891e75ffa869c6f292022-12-22T01:27:48ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062012-05-01562e22e2210.4081/ejh.2012.221267Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid ratsY. ZhuD. NingF. WangC. LiuY. XuX. JiaD. ZhuAdult-onset hypothyroidism induces a variety of impairments on hippocampus- dependent neurocognitive functioningin which many synaptic proteins in hippocampus neurons are involved. Here, we observed the effect of adult-onset hypothyroidism on the expression of syntaxin-1 and munc-18 in the dorsal hippocampus and whether the altered proteins could be restored by levothyroxine (T4) treatment. All rats were separated into 4 groups randomly: hypothyroid group, 5μg T4/100 g body weight (BW) treated group, 20 μg T4/100g BW treated group and control group. The radioimmunoassay kits were applied to assay the levels of serum T3 and T4, and the levels of syntaxin-1 and munc-18 in hippocampus were assessed by immunohistochemistry and Western blot. Both analysis corroborated that syntaxin-1 in the hypothyroid group was significantly higher. Munc-18 was lower in four layers of CA3 and dentate gyrus by immunohistochemistry. After two weeks of treatment with 5 μg T4/100g BW for hypothyroidism, syntaxin-1 levels were completely restored, whereas the recovery of munc-18 only located in two of the four impaired layers. Twenty μg T4/100g BW treatment normalized munc-18 levels. These data suggested that adult-onset hypothyroidism induced increment of syntaxin-1 and decrement of munc-18 in the dorsal hippocampus, which could be restored by T4 treatment. Larger dosage of T4 caused more effective restorations.http://www.ejh.it/index.php/ejh/article/view/1916hypothyroidism, levothyroxine (T4), munc-18, syntaxin-1, hippocampus |
| spellingShingle | Y. Zhu D. Ning F. Wang C. Liu Y. Xu X. Jia D. Zhu Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats European Journal of Histochemistry hypothyroidism, levothyroxine (T4), munc-18, syntaxin-1, hippocampus |
| title | Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats |
| title_full | Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats |
| title_fullStr | Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats |
| title_full_unstemmed | Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats |
| title_short | Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats |
| title_sort | effect of thyroxine on munc 18 and syntaxin 1 expression in dorsal hippocampus of adult onset hypothyroid rats |
| topic | hypothyroidism, levothyroxine (T4), munc-18, syntaxin-1, hippocampus |
| url | http://www.ejh.it/index.php/ejh/article/view/1916 |
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