Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis.
Pseudomonas syringae employs a type III secretion system to inject 20-30 different type III effector (T3SE) proteins into plant host cells. A major role of T3SEs is to suppress plant immune responses and promote bacterial infection. The YopJ/HopZ acetyltransferases are a superfamily of T3SEs found i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0116152 |
| _version_ | 1819148512717176832 |
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| author | Jennifer D Lewis Mike Wilton G Adam Mott Wenwan Lu Jana A Hassan David S Guttman Darrell Desveaux |
| author_facet | Jennifer D Lewis Mike Wilton G Adam Mott Wenwan Lu Jana A Hassan David S Guttman Darrell Desveaux |
| author_sort | Jennifer D Lewis |
| collection | DOAJ |
| description | Pseudomonas syringae employs a type III secretion system to inject 20-30 different type III effector (T3SE) proteins into plant host cells. A major role of T3SEs is to suppress plant immune responses and promote bacterial infection. The YopJ/HopZ acetyltransferases are a superfamily of T3SEs found in both plant and animal pathogenic bacteria. In P. syringae, this superfamily includes the evolutionarily diverse HopZ1, HopZ2 and HopZ3 alleles. To investigate the roles of the HopZ family in immunomodulation, we generated dexamethasone-inducible T3SE transgenic lines of Arabidopsis for HopZ family members and characterized them for immune suppression phenotypes. We show that all of the HopZ family members can actively suppress various facets of Arabidopsis immunity in a catalytic residue-dependent manner. HopZ family members can differentially suppress the activation of mitogen-activated protein (MAP) kinase cascades or the production of reactive oxygen species, whereas all members can promote the growth of non-virulent P. syringae. Localization studies show that four of the HopZ family members containing predicted myristoylation sites are localized to the vicinity of the plasma membrane while HopZ3 which lacks the myristoylation site is at least partially nuclear localized, suggesting diversification of immunosuppressive mechanisms. Overall, we demonstrate that despite significant evolutionary diversification, all HopZ family members can suppress immunity in Arabidopsis. |
| first_indexed | 2024-12-22T13:46:54Z |
| format | Article |
| id | doaj.art-1f24bd50ba17419ab1e047067bd0f137 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T13:46:54Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1f24bd50ba17419ab1e047067bd0f1372022-12-21T18:23:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11615210.1371/journal.pone.0116152Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis.Jennifer D LewisMike WiltonG Adam MottWenwan LuJana A HassanDavid S GuttmanDarrell DesveauxPseudomonas syringae employs a type III secretion system to inject 20-30 different type III effector (T3SE) proteins into plant host cells. A major role of T3SEs is to suppress plant immune responses and promote bacterial infection. The YopJ/HopZ acetyltransferases are a superfamily of T3SEs found in both plant and animal pathogenic bacteria. In P. syringae, this superfamily includes the evolutionarily diverse HopZ1, HopZ2 and HopZ3 alleles. To investigate the roles of the HopZ family in immunomodulation, we generated dexamethasone-inducible T3SE transgenic lines of Arabidopsis for HopZ family members and characterized them for immune suppression phenotypes. We show that all of the HopZ family members can actively suppress various facets of Arabidopsis immunity in a catalytic residue-dependent manner. HopZ family members can differentially suppress the activation of mitogen-activated protein (MAP) kinase cascades or the production of reactive oxygen species, whereas all members can promote the growth of non-virulent P. syringae. Localization studies show that four of the HopZ family members containing predicted myristoylation sites are localized to the vicinity of the plasma membrane while HopZ3 which lacks the myristoylation site is at least partially nuclear localized, suggesting diversification of immunosuppressive mechanisms. Overall, we demonstrate that despite significant evolutionary diversification, all HopZ family members can suppress immunity in Arabidopsis.https://doi.org/10.1371/journal.pone.0116152 |
| spellingShingle | Jennifer D Lewis Mike Wilton G Adam Mott Wenwan Lu Jana A Hassan David S Guttman Darrell Desveaux Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis. PLoS ONE |
| title | Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis. |
| title_full | Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis. |
| title_fullStr | Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis. |
| title_full_unstemmed | Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis. |
| title_short | Immunomodulation by the Pseudomonas syringae HopZ type III effector family in Arabidopsis. |
| title_sort | immunomodulation by the pseudomonas syringae hopz type iii effector family in arabidopsis |
| url | https://doi.org/10.1371/journal.pone.0116152 |
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