Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitals
Background and Aim: Pseudomonas aeruginosa is a unique bacteria that in order to survive in different environments by complex adaptation process can make changes in his virulence genes expression and drug resistance. The aim of this research is the investigation of existence of a logical associatio...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Farname
2014-12-01
|
| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://www.ijmm.ir/browse.php?a_code=A-10-240-1&slc_lang=en&sid=1 |
| _version_ | 1823939746471084032 |
|---|---|
| author | Nour Amirmozafari Jalil Fallah Mehrabadi Khosro Isazadieh Alireza Habibi |
| author_facet | Nour Amirmozafari Jalil Fallah Mehrabadi Khosro Isazadieh Alireza Habibi |
| author_sort | Nour Amirmozafari |
| collection | DOAJ |
| description | Background and Aim: Pseudomonas aeruginosa is a unique bacteria that in order to survive in different environments by complex adaptation process can make changes in his virulence genes expression and drug resistance. The aim of this research is the investigation of existence of a logical association between toxA gene and antibiotic resistance in strains possess the gene.
Materials and Methods: Antibiogram test by disk diffusion method (Kirby Bauer) was performed according to CLSI protocols. In this study, the existence of toxA gene with the help of polymerase chain reaction (PCR) in 102 clinical isolates from blood samples, wound, urine and trachea was examined. Chi-square test was used to investigate the relationship between exotoxin A and antibiotic resistance.
Results: The 81 strains (79.4%) had toxA gene. Frequency of toxA genes in isolated strains from different infections were wound (91.4%), blood (85.7%), trachea (72.7%), and urine (42.1%). Multiple resistance index in strains possess the toxA gene was calculated 75%. Chi 2 test to determine the relationship between drug resistance and gene toxA was significant (P<0.05).
Conclusions: The significant chi-square test and an increase in multi-resistant strains possessing the toxA gene, can represent a considerable genetic switch between exotoxin A activity and resistance to antibiotics in the blood, urine, tracheal, wound infections Respectively, which lead to turn genes on of drug resistance regulating in bacteria. The results of this study will be verified by southern blot, analysis of the expression of toxA gene and determine the mechanism of resistance in resistant strains Methods. |
| first_indexed | 2024-12-17T04:03:50Z |
| format | Article |
| id | doaj.art-1f24e799c062476fa46ab7ad6ff164a3 |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-12-17T04:03:50Z |
| publishDate | 2014-12-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-1f24e799c062476fa46ab7ad6ff164a32022-12-21T22:04:26ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422014-12-01843643Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitalsNour Amirmozafari0Jalil Fallah Mehrabadi1Khosro Isazadieh2Alireza Habibi3 Iran University of Medical Sciences Malek Ashtar University Islamic Azad University Lahijan Branch Department of Microbiology, Faculty of Science, Islamic Azad University, Lahijan Branch, Iran. Background and Aim: Pseudomonas aeruginosa is a unique bacteria that in order to survive in different environments by complex adaptation process can make changes in his virulence genes expression and drug resistance. The aim of this research is the investigation of existence of a logical association between toxA gene and antibiotic resistance in strains possess the gene. Materials and Methods: Antibiogram test by disk diffusion method (Kirby Bauer) was performed according to CLSI protocols. In this study, the existence of toxA gene with the help of polymerase chain reaction (PCR) in 102 clinical isolates from blood samples, wound, urine and trachea was examined. Chi-square test was used to investigate the relationship between exotoxin A and antibiotic resistance. Results: The 81 strains (79.4%) had toxA gene. Frequency of toxA genes in isolated strains from different infections were wound (91.4%), blood (85.7%), trachea (72.7%), and urine (42.1%). Multiple resistance index in strains possess the toxA gene was calculated 75%. Chi 2 test to determine the relationship between drug resistance and gene toxA was significant (P<0.05). Conclusions: The significant chi-square test and an increase in multi-resistant strains possessing the toxA gene, can represent a considerable genetic switch between exotoxin A activity and resistance to antibiotics in the blood, urine, tracheal, wound infections Respectively, which lead to turn genes on of drug resistance regulating in bacteria. The results of this study will be verified by southern blot, analysis of the expression of toxA gene and determine the mechanism of resistance in resistant strains Methods.http://www.ijmm.ir/browse.php?a_code=A-10-240-1&slc_lang=en&sid=1Pseudomonas aeruginosa Exotoxin A Antibiotic resistance |
| spellingShingle | Nour Amirmozafari Jalil Fallah Mehrabadi Khosro Isazadieh Alireza Habibi Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitals Iranian Journal of Medical Microbiology Pseudomonas aeruginosa Exotoxin A Antibiotic resistance |
| title | Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitals |
| title_full | Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitals |
| title_fullStr | Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitals |
| title_full_unstemmed | Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitals |
| title_short | Molecular analysis of exotoxin A associated with antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients in Tehran hospitals |
| title_sort | molecular analysis of exotoxin a associated with antimicrobial resistance of pseudomonas aeruginosa strains isolated from patients in tehran hospitals |
| topic | Pseudomonas aeruginosa Exotoxin A Antibiotic resistance |
| url | http://www.ijmm.ir/browse.php?a_code=A-10-240-1&slc_lang=en&sid=1 |
| work_keys_str_mv | AT nouramirmozafari molecularanalysisofexotoxinaassociatedwithantimicrobialresistanceofpseudomonasaeruginosastrainsisolatedfrompatientsintehranhospitals AT jalilfallahmehrabadi molecularanalysisofexotoxinaassociatedwithantimicrobialresistanceofpseudomonasaeruginosastrainsisolatedfrompatientsintehranhospitals AT khosroisazadieh molecularanalysisofexotoxinaassociatedwithantimicrobialresistanceofpseudomonasaeruginosastrainsisolatedfrompatientsintehranhospitals AT alirezahabibi molecularanalysisofexotoxinaassociatedwithantimicrobialresistanceofpseudomonasaeruginosastrainsisolatedfrompatientsintehranhospitals |