Isolation and Characterization of <i>Bacillus cereus</i> Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages
<i>Bacillus</i> phage φ29 and its relatives have been considered as one of the most important model organisms for DNA replication, transcription, morphogenesis, DNA packaging studies, and nanotechnology applications. Here, we isolated and characterized a new member of the φ29-like phage,...
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/11/1750 |
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| author | Chun Li Xiaoming Yuan Na Li Juan Wang Shubo Yu Haiyan Zeng Jumei Zhang Qingping Wu Yu Ding |
| author_facet | Chun Li Xiaoming Yuan Na Li Juan Wang Shubo Yu Haiyan Zeng Jumei Zhang Qingping Wu Yu Ding |
| author_sort | Chun Li |
| collection | DOAJ |
| description | <i>Bacillus</i> phage φ29 and its relatives have been considered as one of the most important model organisms for DNA replication, transcription, morphogenesis, DNA packaging studies, and nanotechnology applications. Here, we isolated and characterized a new member of the φ29-like phage, named <i>Bacillus cereus</i> phage vB_BceP-DLc1. This phage, with a unique inserted gene cluster, has the largest genome among known φ29-like phages. DLc1 can use the surface carbohydrate structures of the host cell as receptors and only infects the most related <i>B. cereus</i> strains, showing high host-specificity. The adsorption rate constant and life cycle of DLc1 under experimental conditions were also determined. Not only stable under temperatures below 55 °C and pH range from 5 to 11, the new phage also showed tolerance to high concentrations of NaCl, 75% ethanol, chloroform, and mechanical vortex, which is preferable for practical use in the food and pharmaceutical industries. |
| first_indexed | 2024-03-10T15:01:51Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:01:51Z |
| publishDate | 2020-11-01 |
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| series | Microorganisms |
| spelling | doaj.art-1f25a05fb337469dac3a6cccc4f651522023-11-20T20:07:35ZengMDPI AGMicroorganisms2076-26072020-11-01811175010.3390/microorganisms8111750Isolation and Characterization of <i>Bacillus cereus</i> Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like PhagesChun Li0Xiaoming Yuan1Na Li2Juan Wang3Shubo Yu4Haiyan Zeng5Jumei Zhang6Qingping Wu7Yu Ding8Department of Food Science and Technology, Institute of Food Safety and Nutrition, College of Science & Engineering, Jinan University, Guangzhou 510632, ChinaDepartment of Food Science and Technology, Institute of Food Safety and Nutrition, College of Science & Engineering, Jinan University, Guangzhou 510632, ChinaDepartment of Food Science and Technology, Institute of Food Safety and Nutrition, College of Science & Engineering, Jinan University, Guangzhou 510632, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaDepartment of Food Science and Technology, Institute of Food Safety and Nutrition, College of Science & Engineering, Jinan University, Guangzhou 510632, China<i>Bacillus</i> phage φ29 and its relatives have been considered as one of the most important model organisms for DNA replication, transcription, morphogenesis, DNA packaging studies, and nanotechnology applications. Here, we isolated and characterized a new member of the φ29-like phage, named <i>Bacillus cereus</i> phage vB_BceP-DLc1. This phage, with a unique inserted gene cluster, has the largest genome among known φ29-like phages. DLc1 can use the surface carbohydrate structures of the host cell as receptors and only infects the most related <i>B. cereus</i> strains, showing high host-specificity. The adsorption rate constant and life cycle of DLc1 under experimental conditions were also determined. Not only stable under temperatures below 55 °C and pH range from 5 to 11, the new phage also showed tolerance to high concentrations of NaCl, 75% ethanol, chloroform, and mechanical vortex, which is preferable for practical use in the food and pharmaceutical industries.https://www.mdpi.com/2076-2607/8/11/1750<i>Bacillus cereus</i>bacteriophageφ29stabilityadsorptionlargest genome |
| spellingShingle | Chun Li Xiaoming Yuan Na Li Juan Wang Shubo Yu Haiyan Zeng Jumei Zhang Qingping Wu Yu Ding Isolation and Characterization of <i>Bacillus cereus</i> Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages Microorganisms <i>Bacillus cereus</i> bacteriophage φ29 stability adsorption largest genome |
| title | Isolation and Characterization of <i>Bacillus cereus</i> Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages |
| title_full | Isolation and Characterization of <i>Bacillus cereus</i> Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages |
| title_fullStr | Isolation and Characterization of <i>Bacillus cereus</i> Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages |
| title_full_unstemmed | Isolation and Characterization of <i>Bacillus cereus</i> Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages |
| title_short | Isolation and Characterization of <i>Bacillus cereus</i> Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages |
| title_sort | isolation and characterization of i bacillus cereus i phage vb bcep dlc1 reveals the largest member of the φ29 like phages |
| topic | <i>Bacillus cereus</i> bacteriophage φ29 stability adsorption largest genome |
| url | https://www.mdpi.com/2076-2607/8/11/1750 |
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