Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay
OBJECTIVE: To evaluate the Haemoglobin Colour Scale developed by WHO for estimating haemoglobin concentration and to compare the results obtained using it and the HemoCue assay with those determined using a reference method, the Technicon H3 analyser. METHODS: The Colour Scale and HemoCue assay were...
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Format: | Article |
Language: | English |
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The World Health Organization
2002-01-01
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Series: | Bulletin of the World Health Organization |
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Online Access: | http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862002001000010 |
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author | Paddle J.J. |
author_facet | Paddle J.J. |
author_sort | Paddle J.J. |
collection | DOAJ |
description | OBJECTIVE: To evaluate the Haemoglobin Colour Scale developed by WHO for estimating haemoglobin concentration and to compare the results obtained using it and the HemoCue assay with those determined using a reference method, the Technicon H3 analyser. METHODS: The Colour Scale and HemoCue assay were used to test 408 blood samples. Subsequently, Bland-Altman plots were determined and the proximity of the test results to those obtained using the reference method was determined. FINDINGS: The mean difference between the Haemoglobin Colour Scale and the reference method was 0.19 g/dl (95% confidence interval: 3.50 g/dl below to 3.11 g/dl above); the corresponding value for the HemoCue assay was 0.50 g/dl (1.16 g/dl below to 0.16 g/dl above). Only 46.08% of the results obtained by means of the Colour Scale were within 1.0 g/dl of the reference method, whereas 95.34% of the HemoCue results fell within this limit; 22.79% of the Colour Scale results but none of the HemoCue results lay more than 2.0 g/dl from the reference method. CONCLUSION: The Haemoglobin Colour Scale test is too inaccurate for general use, particularly if devices such as the HemoCue are available. |
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institution | Directory Open Access Journal |
issn | 0042-9686 |
language | English |
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publisher | The World Health Organization |
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series | Bulletin of the World Health Organization |
spelling | doaj.art-1f281526be3b45f087dc0cb4622a5a002024-03-03T02:48:47ZengThe World Health OrganizationBulletin of the World Health Organization0042-96862002-01-018010813816Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assayPaddle J.J.OBJECTIVE: To evaluate the Haemoglobin Colour Scale developed by WHO for estimating haemoglobin concentration and to compare the results obtained using it and the HemoCue assay with those determined using a reference method, the Technicon H3 analyser. METHODS: The Colour Scale and HemoCue assay were used to test 408 blood samples. Subsequently, Bland-Altman plots were determined and the proximity of the test results to those obtained using the reference method was determined. FINDINGS: The mean difference between the Haemoglobin Colour Scale and the reference method was 0.19 g/dl (95% confidence interval: 3.50 g/dl below to 3.11 g/dl above); the corresponding value for the HemoCue assay was 0.50 g/dl (1.16 g/dl below to 0.16 g/dl above). Only 46.08% of the results obtained by means of the Colour Scale were within 1.0 g/dl of the reference method, whereas 95.34% of the HemoCue results fell within this limit; 22.79% of the Colour Scale results but none of the HemoCue results lay more than 2.0 g/dl from the reference method. CONCLUSION: The Haemoglobin Colour Scale test is too inaccurate for general use, particularly if devices such as the HemoCue are available.http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862002001000010Hemoglobinometry/methodsReference standardsComparative studyDeveloping countries |
spellingShingle | Paddle J.J. Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay Bulletin of the World Health Organization Hemoglobinometry/methods Reference standards Comparative study Developing countries |
title | Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay |
title_full | Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay |
title_fullStr | Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay |
title_full_unstemmed | Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay |
title_short | Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay |
title_sort | evaluation of the haemoglobin colour scale and comparison with the hemocue haemoglobin assay |
topic | Hemoglobinometry/methods Reference standards Comparative study Developing countries |
url | http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862002001000010 |
work_keys_str_mv | AT paddlejj evaluationofthehaemoglobincolourscaleandcomparisonwiththehemocuehaemoglobinassay |