Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay

OBJECTIVE: To evaluate the Haemoglobin Colour Scale developed by WHO for estimating haemoglobin concentration and to compare the results obtained using it and the HemoCue assay with those determined using a reference method, the Technicon H3 analyser. METHODS: The Colour Scale and HemoCue assay were...

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Main Author: Paddle J.J.
Format: Article
Language:English
Published: The World Health Organization 2002-01-01
Series:Bulletin of the World Health Organization
Subjects:
Online Access:http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862002001000010
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author Paddle J.J.
author_facet Paddle J.J.
author_sort Paddle J.J.
collection DOAJ
description OBJECTIVE: To evaluate the Haemoglobin Colour Scale developed by WHO for estimating haemoglobin concentration and to compare the results obtained using it and the HemoCue assay with those determined using a reference method, the Technicon H3 analyser. METHODS: The Colour Scale and HemoCue assay were used to test 408 blood samples. Subsequently, Bland-Altman plots were determined and the proximity of the test results to those obtained using the reference method was determined. FINDINGS: The mean difference between the Haemoglobin Colour Scale and the reference method was 0.19 g/dl (95% confidence interval: 3.50 g/dl below to 3.11 g/dl above); the corresponding value for the HemoCue assay was 0.50 g/dl (1.16 g/dl below to 0.16 g/dl above). Only 46.08% of the results obtained by means of the Colour Scale were within 1.0 g/dl of the reference method, whereas 95.34% of the HemoCue results fell within this limit; 22.79% of the Colour Scale results but none of the HemoCue results lay more than 2.0 g/dl from the reference method. CONCLUSION: The Haemoglobin Colour Scale test is too inaccurate for general use, particularly if devices such as the HemoCue are available.
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spelling doaj.art-1f281526be3b45f087dc0cb4622a5a002024-03-03T02:48:47ZengThe World Health OrganizationBulletin of the World Health Organization0042-96862002-01-018010813816Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assayPaddle J.J.OBJECTIVE: To evaluate the Haemoglobin Colour Scale developed by WHO for estimating haemoglobin concentration and to compare the results obtained using it and the HemoCue assay with those determined using a reference method, the Technicon H3 analyser. METHODS: The Colour Scale and HemoCue assay were used to test 408 blood samples. Subsequently, Bland-Altman plots were determined and the proximity of the test results to those obtained using the reference method was determined. FINDINGS: The mean difference between the Haemoglobin Colour Scale and the reference method was 0.19 g/dl (95% confidence interval: 3.50 g/dl below to 3.11 g/dl above); the corresponding value for the HemoCue assay was 0.50 g/dl (1.16 g/dl below to 0.16 g/dl above). Only 46.08% of the results obtained by means of the Colour Scale were within 1.0 g/dl of the reference method, whereas 95.34% of the HemoCue results fell within this limit; 22.79% of the Colour Scale results but none of the HemoCue results lay more than 2.0 g/dl from the reference method. CONCLUSION: The Haemoglobin Colour Scale test is too inaccurate for general use, particularly if devices such as the HemoCue are available.http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862002001000010Hemoglobinometry/methodsReference standardsComparative studyDeveloping countries
spellingShingle Paddle J.J.
Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay
Bulletin of the World Health Organization
Hemoglobinometry/methods
Reference standards
Comparative study
Developing countries
title Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay
title_full Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay
title_fullStr Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay
title_full_unstemmed Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay
title_short Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay
title_sort evaluation of the haemoglobin colour scale and comparison with the hemocue haemoglobin assay
topic Hemoglobinometry/methods
Reference standards
Comparative study
Developing countries
url http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862002001000010
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