Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration
Summary: Sympathetic innervation influences homeostasis, repair, and pathology in the cardiac ventricles; in contrast, parasympathetic innervation is considered to have minimal contribution and influence in the ventricles. Here, we use genetic models, whole-mount imaging, and three-dimensional model...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223017868 |
| _version_ | 1797647869483679744 |
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| author | Rebecca J. Salamon Poorva Halbe William Kasberg Jiyoung Bae Anjon Audhya Ahmed I. Mahmoud |
| author_facet | Rebecca J. Salamon Poorva Halbe William Kasberg Jiyoung Bae Anjon Audhya Ahmed I. Mahmoud |
| author_sort | Rebecca J. Salamon |
| collection | DOAJ |
| description | Summary: Sympathetic innervation influences homeostasis, repair, and pathology in the cardiac ventricles; in contrast, parasympathetic innervation is considered to have minimal contribution and influence in the ventricles. Here, we use genetic models, whole-mount imaging, and three-dimensional modeling to define cardiac nerve architecture during development, disease, and regeneration. Our approach reveals that parasympathetic nerves extensively innervate the cardiac ventricles. Furthermore, we identify that parasympathetic and sympathetic axons develop synchronously and are bundled throughout the ventricles. We further investigate cardiac nerve remodeling in the regenerative neonatal and the non-regenerative postnatal mouse heart. Our results show that the regenerating myocardium undergoes a unique process of physiological reinnervation, where proper nerve distribution and architecture is reestablished, in stark contrast to the non-regenerating heart. Mechanistically, we demonstrate that physiological reinnervation during regeneration is dependent on collateral artery formation. Our results reveal clinically significant insights into cardiac nerve plasticity which can identify new therapies for cardiac disease. |
| first_indexed | 2024-03-11T15:22:52Z |
| format | Article |
| id | doaj.art-1f2a10dadd3b4802ac12db390773597c |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T15:22:52Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-1f2a10dadd3b4802ac12db390773597c2023-10-28T05:08:09ZengElsevieriScience2589-00422023-10-012610107709Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regenerationRebecca J. Salamon0Poorva Halbe1William Kasberg2Jiyoung Bae3Anjon Audhya4Ahmed I. Mahmoud5Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USADepartment of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USADepartment of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USADepartment of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USADepartment of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USADepartment of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA; Corresponding authorSummary: Sympathetic innervation influences homeostasis, repair, and pathology in the cardiac ventricles; in contrast, parasympathetic innervation is considered to have minimal contribution and influence in the ventricles. Here, we use genetic models, whole-mount imaging, and three-dimensional modeling to define cardiac nerve architecture during development, disease, and regeneration. Our approach reveals that parasympathetic nerves extensively innervate the cardiac ventricles. Furthermore, we identify that parasympathetic and sympathetic axons develop synchronously and are bundled throughout the ventricles. We further investigate cardiac nerve remodeling in the regenerative neonatal and the non-regenerative postnatal mouse heart. Our results show that the regenerating myocardium undergoes a unique process of physiological reinnervation, where proper nerve distribution and architecture is reestablished, in stark contrast to the non-regenerating heart. Mechanistically, we demonstrate that physiological reinnervation during regeneration is dependent on collateral artery formation. Our results reveal clinically significant insights into cardiac nerve plasticity which can identify new therapies for cardiac disease.http://www.sciencedirect.com/science/article/pii/S2589004223017868Molecular biologyNeuroscienceCell biology |
| spellingShingle | Rebecca J. Salamon Poorva Halbe William Kasberg Jiyoung Bae Anjon Audhya Ahmed I. Mahmoud Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration iScience Molecular biology Neuroscience Cell biology |
| title | Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration |
| title_full | Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration |
| title_fullStr | Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration |
| title_full_unstemmed | Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration |
| title_short | Parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration |
| title_sort | parasympathetic and sympathetic axons are bundled in the cardiac ventricles and undergo physiological reinnervation during heart regeneration |
| topic | Molecular biology Neuroscience Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223017868 |
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