A generalizable nanopore sensor for highly specific protein detection at single-molecule precision
Sensitive and accurate approaches for protein detection have many potential applications. Here the authors show how engineered protein nanopore sensors, consisting of a monobody fused to a single-polypeptide nanopore, can be used for highly specific detection of proteins in complex biofluids.
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-03-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-36944-9 |
| _version_ | 1797864199649492992 |
|---|---|
| author | Mohammad Ahmad Jeung-Hoi Ha Lauren A. Mayse Maria F. Presti Aaron J. Wolfe Kelsey J. Moody Stewart N. Loh Liviu Movileanu |
| author_facet | Mohammad Ahmad Jeung-Hoi Ha Lauren A. Mayse Maria F. Presti Aaron J. Wolfe Kelsey J. Moody Stewart N. Loh Liviu Movileanu |
| author_sort | Mohammad Ahmad |
| collection | DOAJ |
| description | Sensitive and accurate approaches for protein detection have many potential applications. Here the authors show how engineered protein nanopore sensors, consisting of a monobody fused to a single-polypeptide nanopore, can be used for highly specific detection of proteins in complex biofluids. |
| first_indexed | 2024-04-09T22:47:47Z |
| format | Article |
| id | doaj.art-1f2b1191daee43b2bc50ec7144ff89b9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-09T22:47:47Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-1f2b1191daee43b2bc50ec7144ff89b92023-03-22T11:43:52ZengNature PortfolioNature Communications2041-17232023-03-0114111410.1038/s41467-023-36944-9A generalizable nanopore sensor for highly specific protein detection at single-molecule precisionMohammad Ahmad0Jeung-Hoi Ha1Lauren A. Mayse2Maria F. Presti3Aaron J. Wolfe4Kelsey J. Moody5Stewart N. Loh6Liviu Movileanu7Department of Physics, Syracuse UniversityDepartment of Biochemistry and Molecular Biology, State University of New York-Upstate Medical UniversityDepartment of Physics, Syracuse UniversityDepartment of Biochemistry and Molecular Biology, State University of New York-Upstate Medical UniversityDepartment of Physics, Syracuse UniversityDepartment of Physics, Syracuse UniversityDepartment of Biochemistry and Molecular Biology, State University of New York-Upstate Medical UniversityDepartment of Physics, Syracuse UniversitySensitive and accurate approaches for protein detection have many potential applications. Here the authors show how engineered protein nanopore sensors, consisting of a monobody fused to a single-polypeptide nanopore, can be used for highly specific detection of proteins in complex biofluids.https://doi.org/10.1038/s41467-023-36944-9 |
| spellingShingle | Mohammad Ahmad Jeung-Hoi Ha Lauren A. Mayse Maria F. Presti Aaron J. Wolfe Kelsey J. Moody Stewart N. Loh Liviu Movileanu A generalizable nanopore sensor for highly specific protein detection at single-molecule precision Nature Communications |
| title | A generalizable nanopore sensor for highly specific protein detection at single-molecule precision |
| title_full | A generalizable nanopore sensor for highly specific protein detection at single-molecule precision |
| title_fullStr | A generalizable nanopore sensor for highly specific protein detection at single-molecule precision |
| title_full_unstemmed | A generalizable nanopore sensor for highly specific protein detection at single-molecule precision |
| title_short | A generalizable nanopore sensor for highly specific protein detection at single-molecule precision |
| title_sort | generalizable nanopore sensor for highly specific protein detection at single molecule precision |
| url | https://doi.org/10.1038/s41467-023-36944-9 |
| work_keys_str_mv | AT mohammadahmad ageneralizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT jeunghoiha ageneralizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT laurenamayse ageneralizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT mariafpresti ageneralizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT aaronjwolfe ageneralizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT kelseyjmoody ageneralizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT stewartnloh ageneralizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT liviumovileanu ageneralizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT mohammadahmad generalizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT jeunghoiha generalizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT laurenamayse generalizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT mariafpresti generalizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT aaronjwolfe generalizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT kelseyjmoody generalizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT stewartnloh generalizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision AT liviumovileanu generalizablenanoporesensorforhighlyspecificproteindetectionatsinglemoleculeprecision |