Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-de...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
2003-01-01
|
| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018 |
| _version_ | 1819195094998188032 |
|---|---|
| author | Mafra R.A. Leão R.M. Beirão P.S.L. Cruz J.S. |
| author_facet | Mafra R.A. Leão R.M. Beirão P.S.L. Cruz J.S. |
| author_sort | Mafra R.A. |
| collection | DOAJ |
| description | A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons. |
| first_indexed | 2024-12-23T02:07:18Z |
| format | Article |
| id | doaj.art-1f2cea5b70f4488b8b80ae3d4a763ad0 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 0034-7310 |
| language | English |
| last_indexed | 2024-12-23T02:07:18Z |
| publishDate | 2003-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-1f2cea5b70f4488b8b80ae3d4a763ad02022-12-21T18:03:51ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73102003-01-01367951957Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neuronsMafra R.A.Leão R.M.Beirão P.S.L.Cruz J.S.A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018Cerebellar granule neuronsExcitatory amino-acid transporter type 2Patch-clampGlutamateWhole-cell voltage-clamp |
| spellingShingle | Mafra R.A. Leão R.M. Beirão P.S.L. Cruz J.S. Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons Brazilian Journal of Medical and Biological Research Cerebellar granule neurons Excitatory amino-acid transporter type 2 Patch-clamp Glutamate Whole-cell voltage-clamp |
| title | Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons |
| title_full | Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons |
| title_fullStr | Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons |
| title_full_unstemmed | Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons |
| title_short | Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons |
| title_sort | electrophysiological evidence for glial subtype glutamate transporter functional expression in rat cerebellar granule neurons |
| topic | Cerebellar granule neurons Excitatory amino-acid transporter type 2 Patch-clamp Glutamate Whole-cell voltage-clamp |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018 |
| work_keys_str_mv | AT mafrara electrophysiologicalevidenceforglialsubtypeglutamatetransporterfunctionalexpressioninratcerebellargranuleneurons AT leaorm electrophysiologicalevidenceforglialsubtypeglutamatetransporterfunctionalexpressioninratcerebellargranuleneurons AT beiraopsl electrophysiologicalevidenceforglialsubtypeglutamatetransporterfunctionalexpressioninratcerebellargranuleneurons AT cruzjs electrophysiologicalevidenceforglialsubtypeglutamatetransporterfunctionalexpressioninratcerebellargranuleneurons |