In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging
Traditional methods of localizing and quantifying the presence of pathogenic microorganisms in living experimental animal models of infections have mostly relied on sacrificing the animals, dissociating the tissue and counting the number of colony forming units. However, the discovery of several var...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2017.1371897 |
| _version_ | 1818020357638979584 |
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| author | Pinar Avci Mahdi Karimi Magesh Sadasivam Wanessa C. Antunes-Melo Elisa Carrasco Michael R. Hamblin |
| author_facet | Pinar Avci Mahdi Karimi Magesh Sadasivam Wanessa C. Antunes-Melo Elisa Carrasco Michael R. Hamblin |
| author_sort | Pinar Avci |
| collection | DOAJ |
| description | Traditional methods of localizing and quantifying the presence of pathogenic microorganisms in living experimental animal models of infections have mostly relied on sacrificing the animals, dissociating the tissue and counting the number of colony forming units. However, the discovery of several varieties of the light producing enzyme, luciferase, and the genetic engineering of bacteria, fungi, parasites and mice to make them emit light, either after administration of the luciferase substrate, or in the case of the bacterial lux operon without any exogenous substrate, has provided a new alternative. Dedicated bioluminescence imaging (BLI) cameras can record the light emitted from living animals in real time allowing non-invasive, longitudinal monitoring of the anatomical location and growth of infectious microorganisms as measured by strength of the BLI signal. BLI technology has been used to follow bacterial infections in traumatic skin wounds and burns, osteomyelitis, infections in intestines, Mycobacterial infections, otitis media, lung infections, biofilm and endodontic infections and meningitis. Fungi that have been engineered to be bioluminescent have been used to study infections caused by yeasts (Candida) and by filamentous fungi. Parasitic infections caused by malaria, Leishmania, trypanosomes and toxoplasma have all been monitored by BLI. Viruses such as vaccinia, herpes simplex, hepatitis B and C and influenza, have been studied using BLI. This rapidly growing technology is expected to continue to provide much useful information, while drastically reducing the numbers of animals needed in experimental studies. |
| first_indexed | 2024-04-14T08:03:58Z |
| format | Article |
| id | doaj.art-1f2d37bad5314990bb3e2d966d670d5a |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-04-14T08:03:58Z |
| publishDate | 2018-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-1f2d37bad5314990bb3e2d966d670d5a2022-12-22T02:04:48ZengTaylor & Francis GroupVirulence2150-55942150-56082018-12-0191286310.1080/21505594.2017.13718971371897In-vivo monitoring of infectious diseases in living animals using bioluminescence imagingPinar Avci0Mahdi Karimi1Magesh Sadasivam2Wanessa C. Antunes-Melo3Elisa Carrasco4Michael R. Hamblin5Wellman Center for Photomedicine, Massachusetts General HospitalWellman Center for Photomedicine, Massachusetts General HospitalWellman Center for Photomedicine, Massachusetts General HospitalWellman Center for Photomedicine, Massachusetts General HospitalWellman Center for Photomedicine, Massachusetts General HospitalWellman Center for Photomedicine, Massachusetts General HospitalTraditional methods of localizing and quantifying the presence of pathogenic microorganisms in living experimental animal models of infections have mostly relied on sacrificing the animals, dissociating the tissue and counting the number of colony forming units. However, the discovery of several varieties of the light producing enzyme, luciferase, and the genetic engineering of bacteria, fungi, parasites and mice to make them emit light, either after administration of the luciferase substrate, or in the case of the bacterial lux operon without any exogenous substrate, has provided a new alternative. Dedicated bioluminescence imaging (BLI) cameras can record the light emitted from living animals in real time allowing non-invasive, longitudinal monitoring of the anatomical location and growth of infectious microorganisms as measured by strength of the BLI signal. BLI technology has been used to follow bacterial infections in traumatic skin wounds and burns, osteomyelitis, infections in intestines, Mycobacterial infections, otitis media, lung infections, biofilm and endodontic infections and meningitis. Fungi that have been engineered to be bioluminescent have been used to study infections caused by yeasts (Candida) and by filamentous fungi. Parasitic infections caused by malaria, Leishmania, trypanosomes and toxoplasma have all been monitored by BLI. Viruses such as vaccinia, herpes simplex, hepatitis B and C and influenza, have been studied using BLI. This rapidly growing technology is expected to continue to provide much useful information, while drastically reducing the numbers of animals needed in experimental studies.http://dx.doi.org/10.1080/21505594.2017.1371897Bioluminescence imaginginfectious disease pathogenesisluciferasegenetic engineeringbacteriafungivirusesparasites |
| spellingShingle | Pinar Avci Mahdi Karimi Magesh Sadasivam Wanessa C. Antunes-Melo Elisa Carrasco Michael R. Hamblin In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging Virulence Bioluminescence imaging infectious disease pathogenesis luciferase genetic engineering bacteria fungi viruses parasites |
| title | In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging |
| title_full | In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging |
| title_fullStr | In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging |
| title_full_unstemmed | In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging |
| title_short | In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging |
| title_sort | in vivo monitoring of infectious diseases in living animals using bioluminescence imaging |
| topic | Bioluminescence imaging infectious disease pathogenesis luciferase genetic engineering bacteria fungi viruses parasites |
| url | http://dx.doi.org/10.1080/21505594.2017.1371897 |
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